KM0057 – Campylobacter Blood-Free Selective Agar (CCDA)

//KM0057 – Campylobacter Blood-Free Selective Agar (CCDA)

KM0057 – Campylobacter Blood-Free Selective Agar (CCDA)

Campylobacter Blood-Free Selective Medium (CCDA) is one of several media formulations available for the selective isolation of Campylobacter spp., primarily C. jejuni and C. coli. CCDA was described by Bolton et al. and formulated to replace blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate.

CCDA is recommended for food testing. CCDA with the addition of yeast extract and cefoperazone is used in the isolation of Campylobacter spp. from foodstuffs and swabs in the FDA/BAM method. This product complies with the requirements of ISO 10272-1:2006.

Bolton et al. recommended incubating inoculated plates at 37°C to improve isolation rates but incubation at 41.5°C is recommended for the isolation of the commonly encountered thermophilic species (C. jejuni and C. coli).

The meat peptone, beef extract and tryptone provide the required nitrogen, carbon and vitamins. Bacteriological charcoal absorbs toxic compounds and metabolites. Ferrous sulphate and sodium pyruvate are oxygen scavengers. Sodium desoxycholate is a selective agent. Through the addition of campylobacter (Preston) supplement (LS0010), which consists of cefoperazone and amphotericin B, enteric flora is suppressed.

Related Supplements :
LS0010 Campylobacter (Preston) Selective Supplement

SKU: KM0057 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Campylobacter jejuni NCTC 11322 Growth, grey spreading colonies
Campylobacter coli NCTC 11366 Growth, grey spreading colonies
Escherichia coli NCTC 12241 Totally or partially inhibited
Staphylococcus aureus NCTC 12981 Inhibited

 

Formula*

g/L
Meat peptones 10.0
Beef Extract 10.0
Tryptone 3.0
Bacteriological charcoal 4.0
Sodium Chloride 5.0
Sodium desoxycholate 1.0
Ferrous sulphate 0.25
Sodium pyruvate 0.25
Agar 12.0
Total 45.5
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 45.5g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C, add 2 vials of campylobacter (Preston) supplement (LS0010), mix well and aseptically dispense into appropriate sterile containers.