Alkaline peptone water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. This medium may also be used for the enrichment of Vibrio spp. from food and water samples.
First developed by Shread, Donovan and Lee as an enrichment broth for the growth of Aeromonas spp., Cruickshank showed that with a higher pH the medium can be used for the enrichment of Vibrio spp.
The peptone is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance. The high pH of the medium inhibits most enteric organisms for at least 24 hours.
Alkaline Peptone Water
Additional Information
| Shelf Life (days) | |
|---|---|
| Storage Temp (°C) | |
| Dehydrated Medium Appearance | |
| Prepared Medium Appearance | |
| Dehydrated Medium Colour | |
| Prepared Medium Colour | |
| Dehydrated PH | |
| Prepared PH | |
| Recommended Incubation |
Product Description
| Organisms | Ref. No | Result |
| Vibrio paraharmolyticus | NCTC 10885 | Growth |
| Vibrio cholerae | NCTC 11348 | Growth |
Formula*
| g/L | |
| Peptone | 20.0 |
| Sodium Chloride | 20.0 |
| Total | 40.0 |
| *Adjusted/supplemented as required to meet performance requirements |
Preparation
Suspend 40.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.