KM0096 – Clostridium Difficile Agar

//KM0096 – Clostridium Difficile Agar

KM0096 – Clostridium Difficile Agar

Clostridium difficile agar, when supplemented, is used for the isolation of C. difficile from samples. This formulation is a modification of CCFA (Cycloserine-Cefoxitin-Fructose agar) developed by George et al.

The proteose peptone act as carbon, nitrogen and vitamin source in this medium. Fructose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Disodium hydrogen phosphate and potassium dihydrogen phosphate are buffering agents. The magnesium sulfate acts as a source of inorganic ions and the agar is the solidifying agent.
This media requires the addition of defibrinated horse blood and Clostridium difficile selective supplement (LS0022).

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

OrganismRef. No.Result
Clostridium difficileNCTC 11204Growth, grey colonies
Escherichia coliNCTC 12241Inhibited
Staphylococcus aureusNCTC 12981Inhibited

Formula*

FORMULA* g/L
Proteose peptone 40
Fructose6
Disodium hydrogen phosphate 5
Potassium dihydrogen phosphate 1
Magnesium sulfate 0.1
Sodium chloride 2
Agar 14.9
Total 69

Preparation

Suspend 69.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C then aseptically add 7% horse blood and 2 vials of LS0022 Clostridium difficile selective supplement. Mix well then aseptically dispense into appropriate sterile containers.