KM0124 – MacConkey Agar with Salt

//KM0124 – MacConkey Agar with Salt

KM0124 – MacConkey Agar with Salt

This is a selective medium for the isolation and differentiation of bile tolerant Gram-negative (enteric) and Gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology. This medium is recommended by the World Health Organisation and the Department of Health for the bacteriological examination of water.
It has the disadvantage that many strains of Proteus spp. will spread on it and for this reason MacConkey Agar without salt and crystal violet may be preferred (KM0011).

 

SKU: KM0124 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth, pink/red colonies
Proteus mirabilis NCTC 10975 Growth, yellow colonies
Salmonella typhimurium NCTC 12023 Growth, pale colonies
Enterococcus faecalis NCTC 12697 Growth, pink colonies

 

Formula*

g/L
Peptone 20.0
Lactose 10.0
Bile salts 5.0
Sodium chloride 5.0
Neutral red 0.05
Agar 12.0
Total 52.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 52 g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and dispense into appropriate sterile containers.