KM0173 – Urea Broth Base

//KM0173 – Urea Broth Base

KM0173 – Urea Broth Base

A modification of Christensen’s medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. Unlike Christensen’s medium when used for the later purpose it is not necessary to increase the incubation time.
The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator.

Related Supplements :
BM3000 Urea 40% Solution

SKU: KM0173 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli NCTC 12241 Growth, no colour change
Proteus mirabilis NCTC 10975 Growth, medium turns pink

 

Formula*

g/L
Peptone 1.0
Glucose 1.0
Disodium phosphate 1.2
Potassium dihydrogen phosphate 0.8
Sodium chloride 5.0
Phenol red 0.004
Total 9.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 0.9 g of the medium in 95 ml of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C and aseptically add 5ml of sterile 40% urea solution. Dispense into sterile containers.