Mueller Hinton Agar

//Mueller Hinton Agar

Mueller Hinton Agar

SKU: KM0183 Category: Tag:

Mueller Hinton agar is used for antimicrobial susceptibility testing by the disk diffusion method as described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST).
This formulation conforms to Clinical and Laboratory Standard Institute (CLSI). Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium. Starch absorbs any toxic metabolites produced. The medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions.
The base medium can be supplemented with blood (5 – 10%). Nicotinamide adenine dinucleotide (NAD) may be added at 20 mg/L to make the medium suitable for use with more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae (LS0005).

Related Supplements :
LS0005 NAD Supplement (20mgs/L), Defibrinated Horse Blood

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Staphylococcus aureus (no supplementation) ATCC 25923 Growth, white colonies
Escherichia coli (no supplementation) ATCC 25922 Growth, grey colonies
Pseudomonas aeruginosa (no supplementation) ATCC 27853 Growth, cream colonies
Neisseria gonorrhoeae (with 5% horse blood & NAD) NCTC 8375 Growth, grey/opaque colonies
Haemophilus influenzae (with 5% horse blood & NAD) NCTC 8468 Growth, grey/opaque colonies
Streptococcus pneumoniae (with 5% horse blood & NAD) NCTC 12977 Growth, green colonies with α-haemolysis



Beef extract 2.0
Acid hydrolysate of casein 17.5
Starch 1.5
Agar 17.0
Total 38.0
*Adjusted/supplemented as required to meet performance requirements


Suspend 38g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C. If preparing a blood Mueller Hinton plate after cooling aseptically add 50 ml/l of defibrinated horse blood and 2 vials of LS0005. Allow to mix and aseptically dispense into appropriate sterile containers.