Trichomonas Medium

//Trichomonas Medium

Trichomonas Medium

SKU: KM0198 Category:

Trichomonas medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. (1)
The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate.
The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of trichmonads.
Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium.
The addition of selective agents such as chloramphenicol is recommended to inhibit bacterial species that may be present in specimens.

1) Kupferberg, A.B. Johnson, G., and Sprince, H. 1948. Proc. Soc. Exper. Biol. Med., 67:304-308

Additional Information

Shelf Life (days)

Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
Trichomonas vaginalis Wild strain Growth and motile
Escherichia coli ATCC 25922 Inhibited
Staphylococcus aureus ATCC 25923 Inhibited


Recommended incubation: Aerobically at 37 ± 1°C for 48 – 72 hours.


Tryptone 20.0
Liver extract 12.0
Cysteine HCl 1.6
Maltose 1.1
Agar 1.0
Methylene blue 0.003
Total 35.7
*Adjusted/supplemented as required to meet performance requirements


Suspend 35.7g of the medium in one litre of deionised / purified water.
Add 0.1g of chloramphenicol and allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 then cool to 45-50°C.
Once cooled aseptically add 50ml horse serum then dispense into appropriate sterile containers.