Must be stored at 2-8°C

Cefotaxime Selective Supplement E&O Laboratories Ltd Cefotaxime Selective Supplement can be used with MacConkey Agar for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing organisms with the aim of simplifying the differentiation and presumptive identification of Lactose & Non-Lactose fermenting organisms. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. Sodium Chloride is omitted from this medium to provide an electrolyte deficient medium that prevents Proteus spp. from spreading.

Ferric Ammonium Citrate (FAC) Supplement E&O Laboratories Ltd Ferric Ammonium Citrate (LS5004) is a supplement used in the isolation of Listeria spp. with Fraser Broth. Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. The medium is intended for the isolation of Listeria spp. from food and environmental samples when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. It is generally accepted that the USDA-FSIS two stage enrichment method employing UVM primary and secondary enrichment broths is the most suitable for the examination of meat products.

Fraser Broth Selective Supplement E&O Laboratories Ltd Fraser Broth Selective Supplement (LS5002) is an antibiotic supplement used to enhance the selective isolation of Listeria spp. in combination with Fraser Broth.  

Half Fraser Broth Selective Supplement E&O Laboratories Ltd Half Fraser Selective Supplement (LS5003) is selective supplement for the primary enrichment of Listeria spp from food and environmental samples when used with Fraser Broth.Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. It contains ferric ammonium citrate and lithium chloride. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. Fraser Broth has proven to be remarkably accurate in detecting Listeria spp. in food and environmental samples. All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin. Half Fraser Broth is a modification of Fraser Broth which contains half of the concentration of nalidixic acid and acriflavine hydrochloride to aid in the recovery of stressed cells. Half Fraser Broth is used as the primary enrichment broth in the ISO methodology for the detection of Listeria.

Oxytetracycline (100mgs/L) Selective Supplement E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated. Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.