-
This medium is based on the formulation published by Platt, Atherton & Simpson1 and is used for the cultivation of Taylorella equigenitalis, the causative organism in contagious equine metritis. It is routinely used for culturing swabs taken from the genitalia of mares and stallions. Enzymatic digest of casein and soy peptone supply nitrogen, carbon and vitamins and L-cystine is a required growth factor. Sodium chloride provides osmotic balance and sodium sulphite is present as a reducing agent. The medium is made selective by the addition of CEMO Selective Supplement (LS0041) to control bacteria and fungi from swab samples. References 1. Platt, H., Atherton, J. G. and Simpson, D. J. 1978. Equine Vet J 10, 153–159. -
Cetrimide agar is a selective medium for the isolation and detection of Pseudomonas aeruginosa from pharmaceutical, clinical and cosmetic samples. The formulation is complaint with the requirements of the Harmonised USP/EP/JP. Detection is achieved using the unique ability of P. aeruginosa to produce the water soluble, bright green pigment pyocyanin. The production of this pigment is stimulated by the presence of magnesium chloride and di-potassium sulphate in the medium. The addition of glycerol (10ml/l) is required as this compound serves as an energy source. Cetrimide, a quaternary ammonium compound, is also present to suppress the growth of other Pseudomonas spp. as well as Gram-negative and Gram-positive organisms. Pancreatic digest of gelatin provides the required nitrogen, carbon and vitamins. Related Supplements :
-
Charcoal agar is used for the cultivation of fastidious organisms, particularly Bordetella pertussis. Charcoal agar is prepared according to the formulation developed by Mishulow, Sharpe and Cohen. This medium is an efficient substitute for Bordet-Gengou agar in the production of B. pertussis vaccines and can be used as a maintenance medium for stock cultures of Bordetella spp. Beef extract and peptone provide the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance. Starch and charcoal help in absorbing toxic metabolites that are produced during growth of the organism. Nicotinic acid is an essential growth factor for the growth of Bordetella spp. The addition of cephalexin (LS0018) inhibits accompanying contamination in the samples. NB: This is a basic medium only and contains no additional supplement. If cephalexin is added, it should be noted, that although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow. Related Supplements : LS0018 Bordetella Selective Supplement
-
Chromogenic coliform agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique. The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide. Salmon-GAL is used for the detection of ß-D-galactosidase enzymatic activity. X-glucuronide is used for the detection of ß-D-glucoronidase enzymatic activity. β-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies. Unlike other coliforms, Escherichia coli cleaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colonies. Tryptophan is used to increase detection reliability by improving the indole reaction. The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms. Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance. The selectivity is attained by the addition of Tergitol® 7 as it inhibits the growth of Gram-positive bacteria.
-
KM0005 CLED Double Indicator Agar (Bevis) is a differential culture medium used clinically for isolation and enumeration of bacteria in urine, from the suspected cases of urinary tract infection (UTI). KM0005 CLED Double Indicator Agar (Bevis) is a differential culture medium which contains cystine, lactose, bromothymol blue, acid fuchsin and is electrolyte deficient to prevent swarming of Proteus species. Bevis changed the original medium by Mackey and Sandys by introducing a second pH indicator to enhance the differentiation of colony characteristics of lactose and non-lactose fermenting organisms. The medium can allow for quantitative determination of urinary pathogens, including Proteus species, when automated systems or calibrated loops are used for inoculation. KM0005 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with ISO 11133:2014.
-
KM0004 CLED Single Indicator Agar is a differential culture medium used clinically for isolation and enumeration of bacteria in urine, from the suspected cases of urinary tract infection (UTI). Originally described by Sandys, CLED (Cystine Lactose Electrolyte Deficient) Single Indicator Agar is a differential medium which contains cystine, lactose, bromothymol blue and is electrolyte deficient to prevent swarming of Proteus species. The medium can allow for quantitative determination of urinary pathogens, including Proteus species, when automated systems or calibrated loops are used for inoculation. KM0004 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with the principals of ISO 11133:2014.
-
Clostridium difficile agar, when supplemented, is used for the isolation of C. difficile from samples. This formulation is a modification of CCFA (Cycloserine-Cefoxitin-Fructose agar) developed by George et al. The proteose peptone act as carbon, nitrogen and vitamin source in this medium. Fructose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. Disodium hydrogen phosphate and potassium dihydrogen phosphate are buffering agents. The magnesium sulfate acts as a source of inorganic ions and the agar is the solidifying agent. This media requires the addition of defibrinated horse blood and Clostridium difficile selective supplement (LS0022).
-
KM0001 Columbia Agar is a modification of the original Columbia Agar formulation described by Ellner et al. from Columbia University and is tested in accordance with ISO 11133:2014. Columbia Agar provides a medium that is suitable for use with both defibrinated horse and sheep blood to support the growth and determining haemolytic reactions of a variety of microorganisms. Columbia Agar with the addition of plain or chocolated blood is recommended by the UK Standards for Microbiology Investigations for various purposes, including as a standard, supplementary or primary isolation medium. Columbia agar with blood is a general-purpose, non-selective medium suitable for isolation and cultivation of most organisms including fastidious anaerobes from clinical specimens. Chocolating the blood provides a highly nutritious medium which supports the growth of a wide range of pathogens including the most fastidious organisms and is particularly useful for the cultivation of Haemophilus and Neisseria species. Chocolate agar is the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base, which gives the medium a chocolate-brown colouration and from which the agar gets its name. As a result, cell lysis releases intracellular nutrients such as haemoglobin, haemin (X factor), and the coenzyme nicotinamide adenine dinucleotide (NAD or V factor) into the agar. These growth factors are available to fastidious organisms during growth. Related Supplements : Defibrinated Horse Blood, Defibrinated Sheep Blood, Horse Blood Lysed, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficle Selective Supplement, LS0012 Bacitracin Selective Supplement, LS0011 Campylobacter Growth Supplement, LS0009 Campylobacter (Skirrow) Selective Supplement
