KM0001 – Columbia Agar

//KM0001 – Columbia Agar

KM0001 – Columbia Agar

Columbia agar is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms based on the original Columbia agar formulation described by Ellner et al. from Columbia University. With further enrichment using blood, most fastidious organisms can be isolated and their β-haemolytic reactions can be determined in order to aid identification. The peptone is the source of the required nitrogen, carbon and vitamins. Starch increases growth of Neisseria spp., and enhances the haemolytic reactions of some streptococci. This medium is relatively free of reducing sugars, which have been reported to adversely influence the haemolytic reactions of β-haemolytic streptococci . Supplementation of the base medium with blood (5- 10%) will provide additional growth factors for fastidious microorganisms, and aids in determining haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used.

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Defibrinated Horse Blood, Defibrinated Sheep Blood, Horse Blood Lysed, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficle Selective SupplementLS0012 Bacitracin Selective Supplement, LS0011 Campylobacter Growth Supplement, LS0009 Campylobacter (Skirrow) Selective Supplement

SKU: KM0001 Category:

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Escherichia coli (with 5% defibrinated horse blood) NCTC 12241 Growth, grey colonies
Staphylococcus aureus (with 5% defibrinated horse blood) NCTC 12981 Growth, white colonies
Pseudomonas aeruginosa (with 5% defibrinated horse blood) NCTC 12903 Growth, grey colonies
Streptococcus pneumoniae (with 5% defibrinated horse blood) NCTC 12977 Growth, green colonies. α-haemolysis
Streptococcus agalactiae (with 5% deibrinated horse blood) ATCC 12386 Growth, white colonies. β-haemolysis
Listeria monocytogenes (with 5% defibrinated horse blood) NCTC 11994 Growth, white colonies. β-haemolysis
Clostridium sporogenes (without blood and tested under conditions stated in the Harmonised USP/EP/JP) ATCC 19404 Growth, grey colonies

 

Formula*

g/L
Peptones 23.0
Starch 1.0
Sodium chloride 5.0
Agar 11.0
Total 40.0
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 40g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 42-46°C. If required prepare 5-10% Columbia Blood Agar by aseptically adding the appropriate volume of sterile defibrinated horse or sheep blood.