CLED Single Indicator Agar

CLED Single Indicator Agar

KM0004 CLED Single Indicator Agar is a differential culture medium used clinically for isolation and enumeration of bacteria in urine, from the suspected cases of urinary tract infection (UTI).

Originally described by Sandys, CLED (Cystine Lactose Electrolyte Deficient) Single Indicator Agar is a differential medium which contains cystine, lactose, bromothymol blue and is electrolyte deficient to prevent swarming of Proteus species. The medium can allow for quantitative determination of urinary pathogens, including Proteus species, when automated systems or calibrated loops are used for inoculation.

KM0004 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with the principals of ISO 11133:2014.

Additional Information

Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

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Prepared pH

Product Description

Organisms Ref. No Result
Staphylococcus aureus NCTC 12981 Growth: Yellow colonies
Escherichia coli NCTC 12241 Growth: Yellow colonies
Proteus mirabilis NCTC 10975 Growth: Blue colonies (No Swarming)
Pseudomonas aeruginosa NCTC 12903 Growth: Blue colonies
Klebsiella pneumoniae NCTC 9633 Growth: Yellow colonies
Enterococcus faecalis NCTC 12697 Growth: Pale yellow colonies
Proteus vulgaris ATCC 8427 Growth: Blue colonies

 

Formula*

Formula * g/L
Peptone 8.0
Beef extract 3.0
Lactose 10.0
L-Cystine 0.128
Bromothymol blue 0.02
Agar 15.0
Total 36.2
*Adjusted/supplemented as required to meet performance requirements

Preparation

Suspend 36.2g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.