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A general purpose medium enriched with 5% Defibrinated Sheep Blood, suitable for the isolation of most organisms including many fastidious anaerobes. -
This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications. -
Campylobacter Selective (Skirrow) Agar This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. Based on Columbia Agar enriched with Lysed Horse Blood. Polymyxin B, Trimethoprim & Vancomycin are added as the selective agents. Sodium Thiosulphate, Pyruvic Acid and Ferrous Sulphate are also included to enhance the aerotolerance of Campylobacter spp. NB: This medium should be incubated at 42°C to optimise selectivity. -
A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophillus species from a variety of clinical specimens. The media is further enriched with Suplex (Polyvitex) that provides vitamins, amino acids, co-enzymes, glucose and other factors which improve the growth of Neisseria and Haemophillus species. -
A highly nutritious medium enriched with Horse Blood, where the blood has been “chocolated” by heating the medium to 60°C. Suitable for the isolation of most pathogens including the most fastidious and is particularly useful for the cultivation of Haemophilus spp. and Neisseria spp. -
Chocolate Agar with 7% Horse Blood & Bacitacin A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 70°C. Suitable for the isolation of most pathogens including many fastidious organisms the addition of Bacitracin makes it is particularly suitable for the selective isolation of Haemophilus spp. -
CLED Agar Mackey and Sandy’s formulation this medium is popular for Urine Culture in the clinical laboratory. The lack of electrolytes inhibits the spreading of Proteus spp. and Bromothymol Blue indicator allows easy differentiation of Lactose and Non-Lactose fermenting organisms. Cystine is also present to benefit those organisms that have a particular Cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium. -
Columbia Agar is a nutritious general-purpose basal medium capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. However when further enriched with Sterile Blood, which can be “chocolated” if required, the medium is generally used for the isolation of most clinically significant pathogens. The medium can be made selective for various groups of organisms by the addition of a range of antimicrobial supplements. This formulation complies with the Harmonized USP/EP/JP. -
A basic general-purpose blood free medium, capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. -
A general purpose medium enriched with 5% Defibrinated Horse Blood, suitable for the isolation of most organisms including many fastidious anaerobes. -
Columbia Agar Base with 5% Horse Blood & Streptococcal Selective Supplement This is a medium for the selective isolation of Streptococcus spp. from clinical samples. Based on Columbia Agar Base enriched with 5% Horse Blood it is made selective by the addition of Colistin and Oxolinic Acid. -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 5% Sheep Blood, suitable for the isolation of most organisms including most fastidious anaerobes of clinical significance. Many workers claim that β-haemolysis is more readily apparent, particularly in group A streptococci, when Sheep Blood is used in place of Horse Blood. -
Columbia Agar Base with 7% Horse Blood & Gardnerella Supplement This is selective medium for the isolation of Gardnerella vaginalis from clinical samples. Based on Columbia Agar the medium is enriched with 7% Horse Blood and made selective by the addition of Colistin and Nalidixic Acid to suppress other bacteria -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 7% Horse Blood, suitable for the cultivation and maintenance of most organisms including many fastidious anaerobes of clinical significance. -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 7% Sheep Blood, suitable for the isolation of most organisms including most fastidious anaerobes of clinical significance. Many workers claim that β haemolysis is more readily apparent, particularly in group A streptococci, when Sheep Blood is used in place of Horse Blood. -
This is a selective medium for the isolation of Staphylococcus and Streptococcus spp. Based on Columbia Agar, it is enriched by the addition of Sheep Blood (7%) the medium is also made selective by the inclusion of Colistin and Naladixic Acid to suppress the growth of the majority of Gram negative bacteria. The addition of sheep blood the medium allows for good colonial appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. -
Columbia Blood Agar with 5% Defibrinated Horse Blood & Cap Selective Supplement This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 5% Horse Blood the medium is made selective by the inclusion of Colistin and Aztreonam to suppress the growth of the majority of Gram negative bacteria. -
This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 7% Horse Blood the medium is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of Gram negative bacteria. -
A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 60°C. Suitable for the cultivation of most pathogens including many fastidious organisms and is particularly suitable for Haemophilus and Neisseria spp. -
DN'ase Medium DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out. -
DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other staphylococci based on deoxyribonuclease activity. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a colourless zone around the colonies, described as being DNase positive, whereas coagulase negative staphylococci do not produce clearing. This particular formulation can also be used for Streptococci and Serratia. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out. -
Edwards Medium (Modified) with 7% Sheep Blood This is a medium for the selective isolation of streptococci, particularly Streptococcus agalactiae, involved in bovine mastitis. The medium is enriched by the addition of 7% Sheep Blood and made selective by the inclusion of Crystal Violet and Thallous Sulphate. Aesculin is also present and assists in the differentiation of Streptococcus agalactiae, which give rise to blue colonies, from Aesculin positive Group D streptococci which produce black colonies. -
This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth. -
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
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This is one of the large group of media, affectionately known as ‘Peptone Water Sugars’, that are generally used in the screening and/or identification of organisms particularly the enterobacteriacae. A positive fermentation of the substrate is clearly indicated by the medium turning pink due to the inclusion Andrade’s Indicator. -
Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
This is a medium for the isolation and identification of Group B streptococci. The principal of the medium is based on the ability of group B streptococci to produce unique orange/red pigmented colonies when incubated anaerobically, particularly on media containing starch products. This medium is non-selective so other organisms will grow on this medium but they do not produce the characteristic pigment. -
This is a general-purpose medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products as well as being suitable in all areas of bacteriological investigation. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP).The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and, in combination with the Lecithin, ethanol and Sodium Thiosulphate inactivates mercurials, halogens and aldehydes). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
A general purpose broth suitable for the cultivation of most micro-organisms including many fastidious organisms and fungi. It is recommended by the United States Pharmacopoeia for sterility testing of many pharmaceutical products. -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation.
