-
Formulated to ISO 6579, Buffered Peptone Water (BPW) is a pre-enrichment medium designed to help sub-lethally damaged Salmonella spp. recover before introducing them into a selective enrichment medium. Enzymatic digest of casein is the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Di-sodium hydrogen phosphate and potassium di-hydrogen phosphate act as buffers in the medium. This nutritious medium is free from inhibitors and is well buffered to maintain pH 7.0 for the incubation period. Sub-lethal injury to Salmonella spp. occurs in many food processes and this pre-enrichment step greatly increases the chance of their recovery, especially if a low number of cells are present in a sample. -
CLED SI Agar Cystine Lactose Electrolyte Deficient Single Indicator (CLED SI) Agar is based on Mackey and Sandys formulation and is popular for culturing urine specimens in the clinical laboratory. The reduced number of electrolyte level prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue as a pH indicator allows easy differentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement.
-
Lactose broth is used for the performance and confirmation of the Presumptive Test for coliforms in water and dairy samples. This medium is also frequently used as a pre-enrichment medium when testing foods, water samples and dairy products for Salmonella spp. Beef extract and gelatin peptone provide the required carbon, nitrogen and vitamins in this medium. Lactose is a fermentable carbohydrate. Fermentation of lactose is detected by the production of gas
-
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. (1&2) Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium. Starch absorbs any toxic metabolites produced. References (1) Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PA. (2) MacFaddin, J. 1985. Media for isolation cultivation, identification maintenance of medical bacteria. Williams & Williams, Baltimore.
-
Plate Count Agar is formulated to the A.P.H.A. specification developed by Buchbinder et al. This medium is intended for use in food, dairy and water bacteriology to perform Total Viable Counts. Tryptone and yeast extract provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate.
-
This is a selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples. Pseudomonas agar base is on Kings A medium which uses magnesium and potassium salts to enhance pigment production. The gelatine peptone and acid hydrolysed casein acts as a nitrogen, vitamins, and carbon source. Magnesium chloride and potassium sulphate promotes production of pyocyanin. The medium can be made selective for P. aeruginosa by the addition of Pseudomonas CN Supplement (LS0006). Alternatively, the medium can be made selective for Pseudomonas spp. generally by the addition of Pseudomonas CFC Selective Supplement (LS0026). The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism.
-
Tryptone Soya Agar (TSA) is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. TSA is commonly referred to as Soybean-Casein Digest Agar. TSA supplemented with lecithin and Tween 80® is widely used in environmental monitoring. With further enrichment using 5-10% sheep or horse blood, most fastidious organisms can be isolated and their haemolytic reactions can be determined in order to aid identification. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. The tryptone and soy peptone are the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood
-
A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
-
This is a selective medium for the isolation and enumeration of yeasts and moulds in dairy products. It is in according to a typical formulation of The International Organisation for Standardisation (ISO). Yeast extract acts as a source of nitrogen, carbon and vitamins in this medium. Glucose is a fermentable carbohydrate. Although the medium has a low pH it is made more selective by the inclusion of chloramphenicol, an antibiotic selective agent.
