-
Tryptone Soya Agar (TSA) is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. TSA is commonly referred to as Soybean-Casein Digest Agar. TSA supplemented with lecithin and Tween 80® is widely used in environmental monitoring. With further enrichment using 5-10% sheep or horse blood, most fastidious organisms can be isolated and their haemolytic reactions can be determined in order to aid identification. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. The tryptone and soy peptone are the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood -
A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
-
Tryptone yeast extract salts (TYES) agar is a culture medium that can be used as the primary isolation medium for Flavobacterium columnare (1) and F. psychrophilum. Growth of Flavobacterium spp. requires a culture media with a lower nutrient content than that used in general-purpose mediums such as brain heart infusion agar andtryptone soya agar. The tryptone and yeast extract provide the required carbon, nitrogen and vitamins. Calcium chloride and magnesium sulphate provide the required minerals. Agar is a solidifying agent.
-
Tryptose is a mixed enzymatically digested protein. This product can be used in preparing microbiological culture media providing unique nutritional properties useful for cultivating fastidious microorganisms.
-
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. It can also be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. When used for the latter purpose it is necessary to increase the incubation time to as long as 48 hours. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The potassium dihydrogen phosphate is a buffer and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution
-
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. Unlike Christensens medium when used for the later purpose it is not necessary to increase the incubation time. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution
-
Violet Red Bile Agar is a medium for the enumeration of coliform organisms in food and dairy products and conforms to American Public Health Association (APHA). Yeast extract and enzymatic digest of gelatin provides the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44ºC.
-
Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP. Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.
