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Mueller Hinton Agar is recommended for use in the antibiotic disk diffusion method by both the European Committee on Antibiotic Sensitivity Testing (EUCAST) and the Clinical Laboratory Standards Institute (CLSI). The medium contains low levels of divalent metal cations, such as calcium and magnesium, to minimise any interference with certain antibiotic classes e.g. aminoglycosides. Starch is also present to absorb any toxic metabolites that may be formed during growth. The medium is low in thymine & thymidine content and is therefore suitable for use in testing sulphonamides and trimethoprim without the addition of blood. -
Mueller Hinton Agar Chocolate is used for the isolation and cultivation of fastidious bacteria from clinical specimens. It may also be used for the susceptibility testing of Neisseria gonorrhoeae. Mueller Hinton Agar Chocolate is an enriched, non-selective medium on which fastidious and non-fastidious bacteria, including normal flora, will grow. Therefore, it is recommended to inoculate specimens also onto appropriate selective media. The term “fastidious bacteria” relates to bacteria that do not grow or do not grow well on normally used primary isolation media containing sheep blood. -
Approved by the Clinical Laboratory Standards Institute (CLSI) in USA this medium can be considered as an alternative to Iso-Sensitest Agar for antimicrobial sensitivity testing by disc diffusion methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim without the addition of Lysed Blood. -
Approved by the National Committee for Clinical Laboratory Standards (NCCLS) in USA this medium is approved for use in antimicrobial sensitivity testing by the disc diffusion method and is recommended particularly for use with the Bauer-Kirby Technique It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim and controlled to ensure correct zone sizes with Tetracyline and Aminoglycoside antibiotics. It can be considered as an alternative to Iso-Sensitest Agar. This particular formulation has an additional 2% Sodium Chloride added to the medium making it suitable for the detection of resistance to Methicillin in staphylococci and it is included in the recommendations of British Society for Antimicrobial Chemotherapy (BSAC) for this purpose. It is not however recommended for testing of organisms requiring a CO2 enriched environment due to the pH effect on the medium. If incubation in a CO2 enriched environment is essential control organisms should be included to confirm that results have not been altered. -
Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST). This medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Additional supplementation of the Mueller Hinton medium using 5% Horse Blood and 20mg/L of Nicotinamide adenine dinucleotide (NAD) makes it suitable for use with the more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae. -
Mueller-Hinton with 2% Glucose & Methylene Blue (25ml) This medium is intended for use as a means of differentiation of Candida spp. based on Mueller-Hinton Agar base. The medium is modified by the addition of Glucose and Methylene Blue indicator and is the recommended media for the susceptibility testing of Yeasts according to the CLSI M44-A2 document. -
A selective medium for the isolation of fungi, particularly dermatophytes from clinical specimens, Mycological Agar is suitable for use in all areas of Mycology. The medium inhibits most bacteria due to the addition of Chloramphenicol which is added to reduce the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction).
