Brucella broth is a non-selective medium for the cultivation of Brucella spp. and other fastidious microorganisms.
Brucella broth has been developed from the APHA formulation for Albimi broth. (1&2)
The enzymatic digest of casein, enzymatic digest of animal tissues and yeast extract provide the necessary carbon, nitrogen and vitamin sources for this medium.
Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance of the medium.
Sodium bisulfite is added to enhance growth.
(1) Hausler, W. J. (ed.). 1976. Standard methods for the examination of dairy products, 14th ed. American Public Health Association, Washington, D.C.
(2) ISO 10272-1:2006. Microbiology of food and animal feeding stuffs – Horizontal method for detection and enumeration of Campylobacter spp.