Brucella Broth

Brucella Broth

SKU: KM0095 Category:

Brucella broth is a non-selective medium for the cultivation of Brucella spp. and other fastidious microorganisms.
Brucella broth has been developed from the APHA formulation for Albimi broth. (1&2)
The enzymatic digest of casein, enzymatic digest of animal tissues and yeast extract provide the necessary carbon, nitrogen and vitamin sources for this medium.
Glucose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance of the medium.
Sodium bisulfite is added to enhance growth.

(1) Hausler, W. J. (ed.). 1976. Standard methods for the examination of dairy products, 14th ed. American Public Health Association, Washington, D.C.
(2) ISO 10272-1:2006. Microbiology of food and animal feeding stuffs – Horizontal method for detection and enumeration of Campylobacter spp.

Additional Information

Shelf Life (days)


Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
Brucella ovis ATCC 25840 Growth, white colonies
Escherichia coli ATCC 25922 Growth, cream colonies


Recommended incubation: For Brucella spp. – Incubate under CO2 at 37 ± 1oC for 18 – 96 hours. Brucella spp. are classified as Biosafety Level 3 pathogens.


Enzymatic digest of casein 10.0
Enzymatic digest of animal tissues 10.0
Yeast extract 2.0
Sodium chloride 5.0
Glucose 1.0
Sodium bisulfite 0.1
Total 28.0
*Adjusted/supplemented as required to meet performance requirements



Suspend 28.0g of the medium in one litre of deionised / purified water.
Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 minutes.
Once cooled, aseptically dispense into appropriate sterile containers.