Bile Aesculin Agar

//Bile Aesculin Agar

Bile Aesculin Agar

SKU: KM0085 Category:

Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci).
Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex.
The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci.
The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc.

Additional Information


Shelf Life (days)

Storage Temp (°C)

Dehydrated Medium Appearance

Prepared Medium Appearance

Dehydrated Medium Colour

Prepared Medium Colour

Dehydrated PH

Prepared PH

Product Description

Organisms Ref. No Result
Enterococcus faecalis NCTC 12697 Growth, colonies with black centre and blackening of media around colony
Escherichia coli NCTC 12241 Inhibited



Peptone 14.0
Bile Salts 15.0
Ferric Ammonium Citrate 0.5
Esculin 1.0
Agar 14.0
Total 44.5
*Adjusted/supplemented as required to meet performance requirements


Suspend 44.5g of the medium in 1 litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium. Cool to 45-50°C, homogenize gently and dispense into appropriate sterile containers.