Perfringens Agar Base

//Perfringens Agar Base

Perfringens Agar Base

SKU: KM0147 Category:

Perfringens Agar Base is a base medium that allows the enumeration of Clostridium perfringens from food samples.
As a base medium, Perfringens Agar Base can have several supplementary and selective agents added to increase selectivity.
The addition of Eye Yolk Emulsion (BM0140) allows the detection of lecithinase activity as precipitates are formed by C. perfringens. The addition of D-cycloserine (LS0023) can be used to inhibit other facultative anaerobes, as used in Tryptose-Sulphite-Cycloserine (TSC) agar. (1&2)
Alternately, kanamycin and polymyxin B (LS0025) can be used to inhibit other coliforms generally found in food samples, as used in Shahidi-Ferguson Perfringens (SFP) agar.(3)
The tryptose and soy peptone provide the required nitrogen, carbon and minerals.
Yeast extract provide the essential vitamins, including the vitamin B group, needed for growth.
The ferric ammonium citrate and sodium metabisulphite allows the reduction of sulphites to hydrogen sulphide by C. perfringens, which produces black colonies.

(1) ISO- 7937:2004. Microbiology of food and animal feeding stuffs- Horizontal method for the enumeration of Clostridium perfringens – Colony count technique
(2) Harmon, S.M., Kauttar, D.A. and Peeler, J.T. 1971. Improved Medium for Enumeration of Clostridium perfringens. Appl. Microbiol. 22:688-692.
(3) Shahidi S. A. and Ferguson A. R. 1971 New Quantitative, Qualitative, and Confirmatory Media for Rapid Analysis of Food for Clostridium perfringens. Appl. Microbiol. 21:500-506

Additional Information

Shelf Life (days)


Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

With BM0140 and LS0023

Organisms Ref. No Result
Clostridium perfringens NCTC 8237 Growth, black colonies with opaque halo
Escherichia coli NCTC 12241 Inhibition


Recommended incubation: Anaerobically at 37 ± 1°C for 18-24 hours.


Tryptose 15.0
Soy peptone 5.0
Yeast extract 5.0
Sodium metabisulphite 1.0
Ferric ammonium citrate 1.0
Agar 19.0
Total 46.0
*Adjusted/supplemented as required to meet performance requirements



Suspend 46g of the medium in one litre of deionised / purified water.
Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 10 minutes to completely dissolve the medium then cool to 42°C.
If required add BM0140 and LS0025 or LS0023 at this point.
Aseptically dispense into appropriate sterile containers.