Sabouraud Dextrose Agar with Chloramphenicol

SKU: KM0068 Category: Dehydrated Culture Media

Sabouraud dextrose agar with chloramphenicol is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology.
Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1)
The tryptone and meat peptone provides the required nitrogen, carbon and vitamins.
The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.(2)
Chloramphenicol is included to increase the selectivity of the media inhibiting a range of Gram-positive and Gram-negative bacteria.

References
(1) Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.
(2) Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

Additional Information

Shelf Life (days)	1095 days
Storage	Once opened keep powdered medium closed to avoid hydration and use before the expiry date on the label.
Dehydrated Medium Appearance	Fine Powder
Dehydrated Medium Colour	Straw
Dehydrated PH	5.6 ± 0.2
Prepared Medium Appearance	Firm Gel
Prepared Medium Colour	Straw
Prepared PH	5.6 ± 0.2
Container	500G, 5KG, 10KG

Product Description

Organisms	Ref. No	Result
T. Rubrum	NCPF 419	Growth: White fluffy colonies with dark red/brown underside
Aspergillus brasiliensis	NCPF 2275	Growth, white colonies with black pigmentation, pale yellow underside
C. albicans	NCPF 3179	Growth: White colonies
A. fumigates	DSMZ 819	Growth: White colonies with blue/green pigmentation, pale yellow underside
Saccharomyces cerevisiae	NCPF 3178	Growth
Escherichia coli	NCTC 12241	Inhibited

Recommended incubation:
Yeasts and E. coli: Aerobically at 25±1°C for 48 hours
Fungi: Aerobically at 25±1°C for 5-10 days

Formula*

	g/L
Tryptone	5.0
Meat peptone	5.0
Dextrose	40.0
Agar	15.0
Chloramphenicol	0.05

Total	65.0
*Adjusted/supplemented as required to meet performance requirements.

Preparation

Suspend 65.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium.
Cool to 45-50°C and aseptically dispense into appropriate sterile containers.
NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.

Sabouraud Dextrose Agar with Chloramphenicol