Sabouraud dextrose agar with chloramphenicol is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology.
Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1)
The tryptone and meat peptone provides the required nitrogen, carbon and vitamins.
The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.(2)
Chloramphenicol is included to increase the selectivity of the media inhibiting a range of Gram-positive and Gram-negative bacteria.
(1) Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.
(2) Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.