Sabouraud Dextrose Agar with Chloramphenicol

//Sabouraud Dextrose Agar with Chloramphenicol

Sabouraud Dextrose Agar with Chloramphenicol

SKU: KM0068 Category:

Sabouraud dextrose agar with chloramphenicol is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology.
Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1)
The tryptone and meat peptone provides the required nitrogen, carbon and vitamins.
The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties.(2)
Chloramphenicol is included to increase the selectivity of the media inhibiting a range of Gram-positive and Gram-negative bacteria.

(1) Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061.
(2) Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.

Additional Information

Shelf Life (days)


Dehydrated Medium Appearance

Dehydrated Medium Colour

Dehydrated PH

Prepared Medium Appearance

Prepared Medium Colour

Prepared PH


Product Description

Organisms Ref. No Result
T. Rubrum NCPF 419 Growth: White fluffy colonies with dark red/brown underside
Aspergillus brasiliensis NCPF 2275 Growth, white colonies with black pigmentation, pale yellow underside
C. albicans NCPF 3179 Growth: White colonies
A. fumigates DSMZ 819 Growth: White colonies with blue/green pigmentation, pale yellow underside
Saccharomyces cerevisiae NCPF 3178 Growth
Escherichia coli NCTC 12241 Inhibited

Recommended incubation:
Yeasts and E. coli: Aerobically at 25±1°C for 48 hours
Fungi: Aerobically at 25±1°C for 5-10 days


Tryptone 5.0
Meat peptone 5.0
Dextrose 40.0
Agar 15.0
Chloramphenicol 0.05
Total 65.0
*Adjusted/supplemented as required to meet performance requirements.



Suspend 65.0g of the medium in one litre of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes.
Sterilise at 121°C for 15 minutes with frequent stirring to completely dissolve the medium.
Cool to 45-50°C and aseptically dispense into appropriate sterile containers.
NB: Avoid overheating this medium as the acidic pH may result in a softer final medium.