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KM0183 Mueller Hinton Agar is used as a base to make various media for antimicrobial susceptibility testing by the disk diffusion method as described by Bauer-Kirby. First described by Mueller and Hinton, Mueller Hinton Agar has been approved as the definitive medium for antimicrobial susceptibility testing by European Committee on Antimicrobial Susceptibility Testing (EUCAST), Clinical and Laboratory Standards Institute (CLSI) and is compliant with ISO16782:2016. KM0183, when prepared as unsupplemented Mueller Hinton Agar, is suggested by EUCAST and CLSI for non-fastidious organisms such as Enterobacterales, Pseudomonas spp., Stenotrophomonas maltophilia, Acinetobacter spp., Staphylococcus spp., Enterococcus spp., Aeromonas spp., Achromobacter xylosoxidans, Vibrio spp., Bacillus spp. and Burkholderia pseudomallei. KM0183, when prepared supplemented with 5% horse blood and nicotinamide adenine dinucleotide (NAD), is suggested by EUCAST and CLSI for fastidious organisms such as Streptococcus pneumoniae, Streptococcus groups A, B, C and G, Viridans group streptococci, Haemophilus influenzae, Moraxella catarrhalis, Listeria monocytogenes, Pasteurella multocida, Campylobacter jejuni and coli, Corynebacterium spp., Aerococcus sanguinicola and urinae and Kingella kingae. Additionally, according to the UK Standards for Microbiology Investigations, when supplemented appropriately, Mueller Hinton Agar can be used in the process of identifying Haemophilus species and the HACEK group of organisms and Helicobacter species from clinical specimens. Related Supplements : LS0005 NAD Supplement (20mgs/L), Defibrinated Horse Blood -
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. (1&2) Beef extract and acid hydrolysate of casein provides the required nitrogen, carbon and vitamins in the medium. Starch absorbs any toxic metabolites produced. References (1) Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PA. (2) MacFaddin, J. 1985. Media for isolation cultivation, identification maintenance of medical bacteria. Williams & Williams, Baltimore.
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This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella spp. reduce tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional tetrathionate broth the addition of novobiocin (40 mg/l) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 20 ml/l of 2% iodine/iodide solution (BM0946). Once the iodine/iodide solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. Related Supplements : LS0024 Novobiocin Supplement (20mgs/L)
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Mycological agar is a selective medium for the isolation of pathogenic fungi, particularly dermatophytes, from clinical specimens. Enzymatic digest of soybean meal provides the required carbon, nitrogen and vitamins. Glucose is an energy source for the metabolism of fungi. The addition of chloramphenicol further reduces the risk of bacterial contamination when processing material that may be heavily contaminated with coliforms. Cycloheximide should also be added (0.4g/L) to suppress the growth of commensal yeasts and saprophytic fungi.
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A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. When used to prepare agar slopes or agar butts, the medium can be used to maintain control organisms. The peptone provides the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.
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A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. The beef extract, peptone and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains osmotic balance.
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Nutrient Broth No.2 is used for the cultivation of fastidious pathogens and other microorganisms. This general use medium, rich in nutrients, allows the growth of bacteria when there is a low level of cells. The medium is particularly suitable as a secondary growth medium for staphylococci to be tested for coagulase production and also be used for sterility testing of aerobic organisms. Beef extract and peptone provide the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.
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Palcam agar is a selective differential medium for the isolation of Listeria monocytogenes from food, clinical and environmental specimens. The Columbia peptone mix and starch provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. Differentiation of Listeria spp. from enterococci and staphylococci occurs through aesculin hydrolysis and mannitol fermentation. Listeria monocytogenes will hydrolyse aesculin and the associated reaction with the ferric ammonium citrate gives rise to a brown/black precipitate around the colonies. Listeria spp. do not however ferment mannitol. Mannitol fermentation is seen through a colour change of the colony or the area around the colony from red to yellow due to the production of acidic end products. Phenol red is the pH indicator in the medium. Lithium chloride is included to inhibit enterococci. The associated Palcam selective supplement, LS0038, contains polymyxin B, acriflavine and ceftazidime to inhibit other Gram-positive and Gram-negative organisms that may be present in specimens. Related Supplements : LS0038 PALCAM Selective Supplement
