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Columbia agar is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms based on the original Columbia agar formulation described by Ellner et al. from Columbia University. With further enrichment using blood, most fastidious organisms can be isolated and their β-haemolytic reactions can be determined in order to aid identification. The peptone is the source of the required nitrogen, carbon and vitamins. Starch increases growth of Neisseria spp., and enhances the haemolytic reactions of some streptococci. This medium is relatively free of reducing sugars, which have been reported to adversely influence the haemolytic reactions of β-haemolytic streptococci . Supplementation of the base medium with blood (5- 10%) will provide additional growth factors for fastidious microorganisms, and aids in determining haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. Related Supplements : Defibrinated Horse Blood, Defibrinated Sheep Blood, Horse Blood Lysed, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficle Selective Supplement, LS0012 Bacitracin Selective Supplement, LS0011 Campylobacter Growth Supplement, LS0009 Campylobacter (Skirrow) Selective Supplement -
Side 1: Columbia Agar with 5% Horse Blood This is a general-purpose medium enriched with 5% defibrinated horse blood that is suitable for the isolation of most organisms, including many fastidious anaerobes. Side 2: Columbia Agar with 5% Horse Blood and CAP Selective Supplement This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 5% horse blood the medium is made selective by the inclusion of colistin and aztreonam to suppress the growth of the majority of Gram-negative bacteria. -
Side 1: Columbia Blood Agar with 7% Sheep Blood & CNA Supplement This is a selective medium for the isolation of Staphylococcus and Streptococcus spp. Based on columbia agar, it is enriched by the addition of sheep blood (7%) the medium is also made selective by the inclusion of colistin and nalidixic acid to suppress the growth of the majority of Gram negative bacteria. The addition of sheep blood to the medium allows for good colonial appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. Side 2: Sabouraud Dextrose Agar with Chloramphenicol A selective medium for the isolation of yeasts and fungi. Sabouraud dextrose agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria, however in this formulation chloramphenicol (150mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. -
Tryptone Soya Agar (TSA) is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. TSA is commonly referred to as Soybean-Casein Digest Agar. TSA supplemented with lecithin and Tween 80® is widely used in environmental monitoring. With further enrichment using 5-10% sheep or horse blood, most fastidious organisms can be isolated and their haemolytic reactions can be determined in order to aid identification. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. The tryptone and soy peptone are the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood
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A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
