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This is a selective medium for the isolation of Actinomyces spp. from clinical specimens. It is based on Fastidious Anaerobe Agar that has been enriched with 7% defibrinated horse blood and made selective by the addition of nalidixic acid to inhibit most aerobes (particularly Gram-negative bacilli) and metronidazole to suppress other anaerobes. -
This is a 25ml fill selective medium for the isolation of Actinomyces spp from clinical specimens. Based on Fastidious Anaerobe Agar enriched with 7% Horse Blood, the medium has been made selective by the inclusion of Nalidixic Acid to inhibit most aerobes, particularly gram-negative bacilli, and Metronidazole to suppress other anaerobes. -
E&O Laboratories Ltd Actinomycete Selective Supplement (LS0015) is an antibiotic supplement used to enhance the isolation of Actinomyces spp. -
Ampicillin Selective Supplement (100mgs/L) E&O Laboratories Ltd Ampicillin Selective Supplement can be used with LB Agar for selective cultivation of E. coli strains that contain plasmids conferring ampicillin resistance. Ampicillin is an antibiotic used to treat a number of bacterial infections. It is a beta-lactam antibiotic that is part of the amino penicillin family. It is active against many Gram-positive and Gram-negative bacteria.
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B.cepacia Selective Supplement E&O Laboratories Ltd Burkholderia Selective Supplement (LS0125) is an antibiotic supplement used to enhance the isolation of Burkholderia cepacia (formerly known as Pseudomonas cepacia).
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Bacillius cereus Selective Agar (PEMBA) This is a medium for the selective isolation and enumeration of Bacillus cereus in food samples. It is said to be particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. The medium is made selective by the inclusion of Polymixin and Sodium Pyruvate is also present which is said to improve Egg Yolk precipitation and enhance sporulation. As Bacillus cereus is Mannitol Negative the colonies are bluish in colour, due to the presence of the Bromothymol Blue Indicator, with a surrounding precipitate of the same colour due to Lecithinase production (from the Egg Yolk). NB: It should be noted that some Proteus spp. and gram positive cocci may grow on this medium. -
Bacillus Cereus (Mannitol Egg Yolk Polymixin - MYP) Agar Intended for the selective enumeration of Bacillus cereus in food samples the medium uses two reactions (Mannitol fermentation and Lecithinase production) to differentiate Bacillus cereus from other members of the species. As Bacillus cereus is Mannitol Negative the colonies are pink in colour due to the presence of Phenol Red Indicator. Lecithinase production (from the Egg Yolk) is indicated by a white precipitate around the colonies. Polymixin is added as the selective agent to inhibit coliforms. NB: It should be noted that some Proteus spp and gram positive cocci may grow on this medium. -
Bacillus cereus Selective Supplement E&O Laboratories Ltd Bacillus Cereus Selective Supplement (LS0020) is an antibiotic supplement used to enhance the selective isolation of Bacillus cereus from foodstuffs and clinical specimens.
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Bacitracin Selective Supplement E&O Laboratories Ltd Bacitracin Supplement (LS0012) is an antibiotic supplement used to enhance the isolation of Haemophilus species.
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Baird Parker Agar with 5% Egg Yolk Tellurite Baird Parker Agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. The medium is made highly selective by the inclusion of Lithium Chloride and Potassium tellurite. The Potassium tellurite inhibits most coliforms and is reduced by staphylococci giving rise to black colonies. Glycine and Sodium Pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides. NB: Any black colonies (with or without the halo) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. Coagulase Test or Latex Agglutination etc.) -
Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc. -
Bile Aesculin Agar Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc. -
A general-purpose medium enriched with 7% Horse Blood, suitable for the isolation of most organisms including many fastidious anaerobes of clinical significance. -
A general purpose medium enriched with 5% Defibrinated Sheep Blood, suitable for the isolation of most organisms including many fastidious anaerobes. -
Bordetella Selective Supplement Bordetella Selective Supplement LS0018 is an antibiotic supplement used to enhance the culture of Bordetella pertussis.
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An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
Brazier's CCEY Agar with 1% Lysed Horse Blood - Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light. -
This medium is intended for use in the isolation of Salmonella spp, other than Salmonella typhi. Although it can be used as a primary isolation medium, it is not recommended for this purpouse and is generally used for subculture from selective enrichment media. It should be noted that the medium is highly selective and therefore not suited to the isolation of salmonella from samples where numbers may be low. NB: - It is not recommended that this medium is used for the isolation of Salmonella typhi and Shigella spp. -
Brilliant Green Agar This medium is intended for use in the isolation of Salmonellae other than Salmonella typhi. Although it can be used as a primary isolation medium it is not recommended for this purpose and is generally used for subculture from selective enrichment media. It should be noted that the medium is highly selective and therefore not suited to the isolation of Salmonella from samples where the numbers may be low. NB: It is not recommended that this medium be used for the isolation of Salmonella typhi and Shigella spp. -
Brucella Agar is a non-selective medium that is enriched with peptones, 5% sheep blood, haemin and vitamin K to support the growth of fastidious, slow growing, obligately anaerobic micro-organisms. -
Brucella agar is an enriched non-selective medium that supports the growth of fastidious and slow growing, obligately anaerobic micro-organisms due to its content of peptones, dextrose and yeast extract. The medium is supplemented with sheep blood, haemin and vitamin K which provide essential nutrients for certain anaerobes. -
This is a modification of the original Nitrate Reduction Broth which is generally used as one of a series of identification tests for the enterobacteriaceae group of organisms. In addition to allowing the testing of Nitrate Reduction this formulation also contains Agar making it possible to concurrently determine motility. The medium is recommended for use in the confirmatory testing of Clostridium perfringens in water samples. The medium is inoculated by “stabbing” the test organism into the medium, using an inoculating needle or straight wire, and after appropriate incubation motility is demonstrated by diffusion of the organism from the line of inoculation into the medium. The Nitrate Reduction Test is a test for the presence of the enzyme nitrate reductase which, in the presence of an appropriate electron donor, reduces nitrate to nitrite. Following incubation “Nitrate Reagent” is added to the medium and a positive reaction is indicated by the formation of a red colour. For full details of the test method reference should be made to appropriate publications. -
This is a selective medium for the isolation of Burkholderia cepacia. The base contains Bile Salts and Crystal Violet as selective agents and Ticarcillin and Polymixin B are added as additional supplements to further improve the selectivity particularly inhibition of most Pseudomonas spp. -
Camplobacter (CCDA) Selective Supplement E&O Laboratories Ltd Campylobacter (CCDA) Selective Supplement (LS0010) is an antibiotic supplement used to enhance the selective isolation of Campylobacter spp. from clinical, food and environmental samples.
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Campylobacter (Skirrow) Selective Supplement E&O Laboratories Ltd Campylobacter (Skirrow) Selective Supplement (LS0009) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
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This medium is designed for the selective isolation of Campylobacter spp., particularly C. jejuni and C. coli , which are major causes of gastrointestinal infection. Campylobacter Blood Free Agar CCDA was formulated to allow replacement of blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. Selectivity is achieved through the addition of broad spectrum cefoperazone and amphotericin B to inhibit fungi. Inoculated plates are incubated at 41.5°C to improve growth of the thermophilic species including C. jejuni and C. coli. Campylobacter Blood Free Agar CCDA is recommended for food testing in compliance with the requirements of ISO 10272- 1:2017(1) . It can also be used for isolation of Campylobacter spp. from clincal specimens such as faeces(2,3). 1. ISO 10272-1:2017. Microbiology of food and animal feeding stuffs – Horizontal method for detection and enumeration of Campylobacter spp. – Part 1: Detection method. 2. Public Health England. (2014). Investigations of Faecal Specimens for Enteric Pathogens. UK Standards for Microbiology Investigations. B 30 Issue 8.1. 3. Public Health England. (2018). Identification of Campylobacter species. UK Standards for Microbiology Investigations. ID 23 Issue 3.1. -
PP0130 Campylobacter Blood Free CCDA is designed for the selective isolation of Campylobacter spp., particularly C. jejuni and C. coli, which are major causes of gastrointestinal infection. Campylobacter Blood Free CCDA was described by Bolton et al. and formulated to replace blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate. This product is recommended for the isolation of Campylobacter spp. and complies with the requirements of ISO 10272-1:2017, ISO 10272-2:2017, is tested in accordance with ISO 11133:2014, and is a recommended medium in the UK Standards for Microbiology Investigations for isolation of Campylobacter spp. from clinical specimens such as faeces. -
This is one of a number of selective enrichment broths that can be used in the isolation of Campylobacter spp from clinical, food and environmental specimens and contains nutrients to aid in the resuscitation of damaged organisms. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Vancomycin, Cefoperazone, Trimethoprim and Amphotericin B. Following incubation at 37ºC the broth is usually sub-cultured onto an appropriate solid Campylobacter medium. -
Campylobacter Growth Supplement E&O Laboratories Ltd Campylobacter Growth Supplement (LS0011) is a supplement used to enhance the isolation and aerotolerance of Campylobacter spp and can be used in both solid and liquid media and generally used in conjunction with media prepared to Skirrow, Butzler or Preston formulations.
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Campylobacter Selective Agar Preston Supplement This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Cefoperazone, to suppress other enteric organisms, and Amphotericin to suppress yeast & fungal growth. -
Campylobacter Selective (Skirrow) Agar This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. Based on Columbia Agar enriched with Lysed Horse Blood. Polymyxin B, Trimethoprim & Vancomycin are added as the selective agents. Sodium Thiosulphate, Pyruvic Acid and Ferrous Sulphate are also included to enhance the aerotolerance of Campylobacter spp. NB: This medium should be incubated at 42°C to optimise selectivity. -
Cefotaxime Selective Supplement E&O Laboratories Ltd Cefotaxime Selective Supplement can be used with MacConkey Agar for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing organisms with the aim of simplifying the differentiation and presumptive identification of Lactose & Non-Lactose fermenting organisms. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. Sodium Chloride is omitted from this medium to provide an electrolyte deficient medium that prevents Proteus spp. from spreading.
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Cetrimide Agar is intended primarily for use in the isolation of Pseudomonas aeruginosa from pharmaceutical products and is recommended by the United States Pharmacopoeia for this purpose. The medium is made selective by the addition of cetrimide to inhibit the growth of most other organisms while allowing Pseudomonas aeruginosa/ and other Pseudomonas spp. to develop a classical colonial appearance, producing green pigmentation and fluoresce when examined under ultra violet light. -
This is a medium intended for the cultivation and isolation of Bordetella pertussis & Haemophilus spp. The base medium contains Charcoal and is enriched with 10% Horse Blood. It can also be used as a maintenance or transport medium for these organisms. -
Charcoal Agar with 10% Horse Blood & Cephalexin This is one of two media generally used for the selective isolation of Bordetella pertussis. The medium is made selective by the inclusion of Cephalexin, to suppress the unwanted naso-pharyngeal flora often present in specimens submitted for the isolation of Bordetella pertussis, and further enriched with 10% Horse Blood. NB: Although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow. -
Chloramphenicol Selective Supplement (100mgs/L) E&O Laboratories Limited Chloramphenicol Selective Supplement (LS0050) is an antibiotic supplement used for the selective isolation of yeasts and moulds from food, environmental and clinical specimens.
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A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophillus species from a variety of clinical specimens. The media is further enriched with Suplex (Polyvitex) that provides vitamins, amino acids, co-enzymes, glucose and other factors which improve the growth of Neisseria and Haemophillus species. -
A highly nutritious medium enriched with Horse Blood, where the blood has been “chocolated” by heating the medium to 60°C. Suitable for the isolation of most pathogens including the most fastidious and is particularly useful for the cultivation of Haemophilus spp. and Neisseria spp. -
Chocolate Agar with 7% Horse Blood & Bacitacin A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 70°C. Suitable for the isolation of most pathogens including many fastidious organisms the addition of Bacitracin makes it is particularly suitable for the selective isolation of Haemophilus spp. -
CLED Agar Mackey and Sandy’s formulation this medium is popular for Urine Culture in the clinical laboratory. The lack of electrolytes inhibits the spreading of Proteus spp. and Bromothymol Blue indicator allows easy differentiation of Lactose and Non-Lactose fermenting organisms. Cystine is also present to benefit those organisms that have a particular Cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium. -
CLED Agar (Bevis) A modification by Bevis of the original CLED medium of Mackey & Sandys. This formulation uses a double indicator system (Andrade’s (Acid fuchsin) and Bromothymol blue) to improve differentiation of Lactose and Non-lactose fermenting organisms. The lack of Sodium Chloride also prevents the swarming of Proteus spp. -
Clostridium difficile Selective Supplement E&O Laboratories Ltd Clostridium Difficile Selective Supplement (LS0022) is an antibiotic supplement used to enhance the isolation of Clostridium difficile from faecal specimens.
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E&O Laboratories Ltd Clostridium perfringens (TSC) Selective Supplement (LS5005) is an antibiotic supplement used to enhance the selective isolation of Clostridium perfringens from clinical specimens and foodstuffs.
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Chromogenic medium for detection of Clostridium difficile. Clostridium difficile (C.difficile) is the leading cause of nosocomial infectious diarrheoa in adults. These infections occur mostly in patients who have both medical care and antibiotic treatment and have become more frequent and more difficult to treat in the last years due to the emergence of highly toxigenic C.difficile strains. Although PCR has become the leading C.difficile detection technique, culture is essential for strain typing and antimicrobial susceptibility testing. CHROMagarTM C.difficile is a new fluorogenic culture medium, extremely sensitive and selective, especially designed to simplify and speed up (24h) the culture of C.difficile. -
In recent years there has been an increase in the number of immuno-compromised patients, which has in turn led to an increased rate of infections associated with Candida species. There were 2151 reported cases of candidaemia in 2016 with C.albicans accounting for 42%, C.glabrata for 25%, C.parapsilosis for 9% and C.tropicalis for 3% of infections in England, Wales and Northern Ireland.(1) COLOREX™ Candida was formulated specifically for the detection and isolation of clinically significant Candida spp. by means of colonial colour and morphology within 48hrs. COLOREX™ Candida allows for the recognition of a minor Candida population within a mixed population as well as the pre-dominant species thereby allowing for a patient specific treatment plan at the earliest possible opportunity. Most bacterial species will be inhibited due to the inclusion of chloramphenicol. C.albicans – Green colonies C.tropicalis – Metallic blue colonies C.glabrata – Mauve to pink colonies C.krusei – Large fuzzy pink colonies Limitations Definitive identification requires additional testing of isolates (e.g. MALDI-TOF). C.glabrata and C.parapsilosis cannot be readily distinguished on this particular medium. C.dubliniensis will form dark green colonies on COLOREX™ Candida so additional testing is required to confirm presence in the specimen. C.auris isolates will grow on this medium but the colony colour may vary from white to pale purple/pink so further testing will be required to confirm identification. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the Enterobacteriaceae. Colorex™ ESBL (Extended Spectrum Beta-Lactamase) medium has been developed for the isolation of ESBL – producing organisms with the aim of simplifying the differentiation and presumptive identification of the causative organism. It should be noted that other non-ESBL and AmpC isolates will be inhibited on this medium reducing the incidence of false positives. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. Typical colour reactions are as follows: Escherichia coli – Red colonies; Proteus spp., Providencia spp. & Morganella spp. – Clear colonies with a brown halo; Klebsiella spp., Enterobacter spp, Serratia spp. & Citrobacter spp. – Metallic blue colonies; Salmonella spp. & Acinetobacter spp. – Clear colonies; Gram +ve bacterial species and yeasts – Inhibited. -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. Colorex™ UTI has been developed primarily for use in the examination of urine specimens with the aim of simplifying the differentiation and presumptive identification of the main organisms, gram negative and gram positive, usually found in Urinary Tract Infections. It can however be used to differentiate organisms in other types of clinical specimens. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium. -
Colorex™ Salmonella Plus is a chromogenic media for the isolation and presumptive identification of Salmonella spp, S typhi, S.paratyphi and lactose positive Salmonella from foodstuffs. Any presumptive Salmonella spp. will produce a mauve colouration; other organisms will be blue or colourless in appearance. Cefsulodin is added as a selective agent to inhibit the growth of Pseudomonas and Aeromonas species. Any presumptive isolates must be confirmed using serological and biochemical techniques available to the laboratory. This product meets the requirements of ISO 6579:2002 standard. -
Traditional methods for the isolation of Vibrio spp. (e.g. TCBS medium) are labour intensive and not particularly sensitive. Colorex™ Vibrio allows for the easy differentiation of V.parahaemolyticus from V.cholerae and V.vulnificus and other Vibrio spp. at the initial isolation stage while retaining a higher level of sensitivity than conventional methods. V.parahaemolyticus produces colonies with a mauve colouration while V.cholerae and V.vulnificus produce colonies with a blue colouration. Colorex™ Vibrio is a highly selective medium with most major Enterobacteriaceae spp. and Gram positive organisms being inhibited during incubation. -
Side One: Columbia Agar & Horse Blood - A general purpose medium enriched with 5% Defibrinated Horse Blood, suitable for the isolation of most organisms, including many fastidious anaerobes. Side Two: Bacitracin Chocolate Agar - A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 60°C. The addition of the selective agent, Bacitracin, makes this medium particularly suitable for the selective isolation of Haemophilus spp.
