Clinical & Veterinary Bottled & Bagged Media List
Bottled & Bagged ready to use culture media for the food, water and environmental microbiological testing laboratories: Broths, agars and diluents are dispensed into various containers and volumes using both manual and fully automated microbiology laboratory equipment production lines. An extensive range of containers/volumes and microbiological media formulations are on offer to meet your individual requirements.
View Our Bottled & Bagged MediaList
-
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. NB: this media will NOT isolate M.bovis which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp. Designed for use with NaOH treated samples which have sterile distilled water added at the end of the designated treatment time to "dilute" the effect of NaOH. This media will isolate most common mycobacteria including M.bovis. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
Alkaline Peptone Water is generally used as an enrichment medium in the isolation of Vibrio spp. from faeces. The high pH of the medium inhibits most enteric organisms for at least 24 hours. The medium is heavily inoculated with faeces and after not more than 8 hours incubation a loopful from the top of the medium is sub cultured onto TCBS Agar. This enrichment medium is also used for food and water testing. -
Bile Aesculin Medium is generally used for the differentiation and presumptive identification of Group D streptococci (enterococci). Enterococci hydrolyse aesculin forming, amongst other products, aesculetin which in turn combines with Ferric ammonium citrate producing a dark brown or black complex. The presence of Bile salts in the medium inhibits gram positive organisms other than enterococci. The medium can also be used for the presumptive identification of certain organisms within the Enterobacteriaceae group such as Klebsiella spp., Enterobacter spp., etc. -
This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria. -
An enriched general purpose broth enriched with 10% defibrinated horse blood for the isolation of fastidious organisms. -
A very nutritious isotonic general-purpose medium with a low concentration of Glucose to stimulate early growth, Brain Heart Infusion Broth is suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. A phosphate buffer is incorporated to help neutralise any acids produced as a result of Glucose utilisation and thus maintain viability of the organisms. This particular formulation also has added Glycerol to act as cryopreservative if the medium is used for the long term frozen storage of microorganisms. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
An enriched general purpose broth being an isotonic medium with Tryptose providing a wide range of substrates. The medium is further enriched with 10% horse serum for more fastidious organisms and as an enrichment broth. -
BM0070 Brain Heart Infusion Broth is a nutritious, isotonic, general-purpose medium suitable for the isolation of most micro-organisms including many fastidious organisms and, with enrichment as appropriate, is suitable as a base for blood culture medium. Modern BHI typically uses an infusion from porcine brains and hearts rather than calf brain tissue, and uses disodium phosphate as a buffer, rather than the calcium carbonate used by Rosenow and Haden. It is a nutrient-rich medium and can therefore be used to culture a variety of fastidious organisms, including streptococci, pneumococci, and meningococci, which can be challenging to grow. Brain Heart Infusion Broth is recommended by the FDA BAM for the enrichment for pathogenic Escherichia coli in foods and environmental samples, and as a medium used in the coagulase test for the identification of Staphylococcus aureus from foods, and Gram-positive cocci from cosmetic samples. NB: Organisms that produce large amounts of acid in the medium may overwhelm the buffering system and as a result ‘auto-sterilise’ the culture. -
Brucella Broth was developed to cultivate Brucella spp. from a wide variety of clinical samples but it is also widely used as a general enrichment broth for both fastidious and non-fastidious organisms. -
This is one of a number of selective enrichment broths that can be used in the isolation of Campylobacter spp from clinical, food and environmental specimens and contains nutrients to aid in the resuscitation of damaged organisms. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Vancomycin, Cefoperazone, Trimethoprim and Amphotericin B. Following incubation at 37ºC the broth is usually sub-cultured onto an appropriate solid Campylobacter medium. -
The principle use for this product is in the testing of disinfectants and antiseptics. In 1989, the European Committee for Standardisation (CEN) set up a technical committee to produce harmonised test methods for disinfectants and antiseptics. The CEN standards provide a useful basis for disinfectant validation, and although alternative methods could be used for assessing disinfectant efficacy, following the same basic methods allows not only direct comparison between products but also comparison across various different laboratories. The adaptability of the methods - numerous validation studies based on the CEN methods have been accepted by both the European and US regulatory authorities - allows end- users to customise the methods to their specific requirements. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). L-histidine, in combination with lecithin and Tween 80, neutralises aldehydes and formaldehyde generating agents. Sodium thiosulphate neutralises iodine and chlorine.
-
Columbia Agar is a nutritious general-purpose basal medium capable of supporting the growth of most micro-organisms, including many fastidious organisms that do not require blood. However when further enriched with Sterile Blood, which can be “chocolated” if required, the medium is generally used for the isolation of most clinically significant pathogens. The medium can be made selective for various groups of organisms by the addition of a range of antimicrobial supplements. This formulation complies with the Harmonized USP/EP/JP. -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 7% Horse Blood, suitable for the cultivation and maintenance of most organisms including many fastidious anaerobes of clinical significance. -
A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 60°C. Suitable for the cultivation of most pathogens including many fastidious organisms and is particularly suitable for Haemophilus and Neisseria spp. -
BM0110 Cooked Meat with Brain Heart Infusion Broth Overlay is suitable for recovery, isolation, and storage of the most fastidious anaerobes, such as Clostridium species as well as many aerobes. Cooked Meat Medium prepared from heart tissue is a well-established medium for the cultivation of anaerobic and aerobic organisms. Cooked Meat with Brain Heart Infusion Broth Overlay has been shown to initiate bacterial growth from very small innocua and to maintain the viability of cultures over long periods of time; it is especially useful in culturing from blood samples. Mixed cultures of bacteria survive without displacing the slower growing organisms. The products of growth do not rapidly destroy the inoculated organisms and therefore it is an excellent medium for the storage of aerobic and anaerobic bacteria. -
For in vitro diagnostic use. BM2250 Cooked Meat with Fastidious Anaerobic Broth Overlay is designed for the culture of fastidious anaerobes, particularly Bacteroides and Clostridium species. Fastidious Anaerobe Broth is formulated from a range of nutrients which have been selected to optimise the recovery and growth of most anaerobic organisms of clinical importance. The nutritious formulation also allows growth from a small initial inoculum which can be important for clinical specimens. Anaerobic bacteria can infect deep wounds, tissue, and internal organs where there is little oxygen after injury or surgery. Infection by anaerobic bacteria is normally characterized by formation of abscesses, foul smelling discharge, pus, and free gas in tissue, leading to tissue degradation. BM2250 is recommended as part of the clinical process for the investigation of these infections (1). BM2250 is also used to cultivate and maintain anaerobic bacteria especially Bacteroides and Clostridium spp. It can differentiate saccharolytic from proteolytic strains. Proteolytic organisms cause degradation of the meat granules and blackening of the meat surface. -
This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of Nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of yeasts. It has also been used for the identification of Candida albicans by chlamydospore production. -
For in vitro diagnostic use. BM0650 Deionised Water is used as a diluent for, where required, the preparation of clinical specimens and microbiological samples, the dilution of microorganisms and reconstitution of lyophilised antibiotic supplements during culture media preparation. Deionised Water is purified water that can be used as a general-purpose diluent in many areas of the laboratory. It may be used for creating 0.5 McFarland suspensions of bacterial isolates for identification tests or processing clinical specimens or industrial samples prior to microbiological examination. -
For in vitro diagnostic use. BM0655 Distilled Water can be used in the preparation of bacterial suspensions and as a diluent in areas of the laboratory. Distilled Water is manufactured from the distillation of deionised water. During this process almost all the impurities in the water are removed and this is why distilled water is chosen for specific laboratory applications. -
Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 "Water quality-Detection and enumeration of Legionella" for this purpose. -
For in vitro diagnostic use. BM1682 Faecal Transport Solution is recommended for the transport of clinical specimens, especially those associated with enteric pathogens (1). This formulation allows for transport at chilled or ambient temperatures.
BM1682 Faecal Transport Solution is a modified Cary Blair Transport Medium (2), designed for the transportation and preservation of clinical specimens, primarily stool and rectal swabs. The product is designed to maintain the viability of enteric bacterial pathogens during transport and subsequent storage at chilled or ambient temperatures.
Faecal Transport Solution is an isotonic, buffered, and nonnutritive medium with a carefully balanced composition. It includes agar to help reduce oxygen diffusion, sodium thioglycolate to impede oxidation, and disodium phosphate to help maintain pH. The relatively high pH of the medium helps to minimises overgrowth of non-target organisms and prevent acid formation in the specimen. References1. CLSI M40-A2. (2014). Quality control of microbiological transport systems; Approved standard – _second edition. CLSI document M40-A2. Wayne, PA: Clinical and Laboratory Standards Institute. 2. Cary, S.G. and Blair, E.B. (1964). New Transport Medium for Shipment of Clinical Specimens. J. Bact, 88:96-98.
-
BM0160 Fastidious Anaerobe Broth is designed for the culture of fastidious anaerobes, particularly Bacteroides species, and is also suitable for use as an anaerobic blood culture medium. BM0160 is recommended by the UK Standards for Microbiology Investigations and is formulated and tested in compliance with the principals of ISO 11133:2014. Peptone and yeast extract supply the required carbon, nitrogen, and vitamins. Haemin, vitamin K, L-arginine, and L-cysteine HCl are additional growth factors required by some anaerobes. Sodium thioglycolate and L-cysteine HCl help lower the pH of the medium to maintain anaerobicity and starch and sodium hydrogen carbonate help neutralise toxins. The increased viscosity produced by addition of agar helps prevent oxygen diffusion in the final medium. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.
-
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
A medium for the selective isolation of group B streptococci. This information has been obtained directly from the HPA files at the customers request. -
This is a chemical complex which should be used in conjunction with BM0945 Muller – Kauffmann Tetrathionate Broth Base. It is recommended that the complex is used as a 2% solution with the base media and should be only added on the day of use. -
Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing. -
Product description to follow.
-
Luria Bertani broth (LB Broth) is a nutrient broth primarily used for the growth and maintenance of Escherichia coli. Used as the primary propagation step for donor or recipient cells, when further work is to be performed on LB Agar. -
Letheen Broth with Neutraliser and 1% Tween 80 is primarily intended for use in assessing the bactericidal activity of quaternary ammonium compounds and determining the phenol coefficient of cationic surfactants. It can also be used in environmental testing, particularly in areas subjected to surface disinfection. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol).
-
LIM Broth with 10% Serum A nutritious, selective broth medium utilising the base formulation developed by Todd and Hewitt for the enrichment of Group B Streptococci. The LIM broth is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of other bacteria. Serum is also included to enhance the nutritional qualities of the base medium. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis, from clinical samples. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein Jensen Medium slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium -
Lowenstein Jensen Pyruvate Medium is an egg-based medium for the cultivation of Mycobacterium spp., particularly Mycobacterium bovis. Lowenstein Jensen Pyruvate Medium is based on the original formulation of Löwenstein that was later modified by Jensen. It differs from Lowenstein Jensen Medium (BM0200) in that sodium pyruvate has replaced the glycerol, which has been demonstrated to be inhibitory to some species, particularly M. bovis. Lowenstein Jensen Pyruvate Medium can be used for the diagnosis of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy). It is recommended by Public Health England as a standard media for the investigation of specimens for Mycobacterium species. The coagulation of the egg albumin during preparation provides a solid surface for inoculation purposes. L-Asparagine and soluble starch are sources of nitrogen and vitamins. Potassium di-hydrogen phosphate and magnesium sulphate enhance organism growth and act as buffers. Sodium pyruvate is added to serve as a carbon source in place of glycerol. The egg mixture also provides essential fatty acids and protein to support the growth of mycobacteria. Malachite green is incorporated into the medium to exert an inhibitory effect on organisms other than mycobacteria.
-
This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth.
-
BM0370 Selenite Mannitol Broth is a selective enrichment medium used for the isolation of Salmonella species from clinical, food and environmental specimens. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media. Selenite Mannitol Broth is recommended for the enrichment of Salmonella species from faecal specimens in UK SMI S7 for the investigation of gastroenteritis. -
This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
This medium is recommended by BSI and ISO for the enumeration of viable organisms in milk and other dairy products. It can also be used as a general-purpose medium for the cultivation of most organisms, particularly those that are less fastidious in their nutritional requirements.
-
Modified Semi Solid Rappaport Vassiliadis Medium (MSRV) is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in faeces and food products. The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment. The semi-solid medium allows motility to be detected as halos of growth around the original point of inoculation.
-
MSS is a viral transport solution designed to facilitate collection and transport of viruses safely from site of sampling to a laboratory location at ambient temperatures. The medium is buffered to help maintain a pH of 7.1, and contains a metal ion chelator, a non-ionic surfactant and a chaotropic agent to ensure disruption of virus particles, denaturation of viral proteins and inactivation of nucleases that may destroy target nucleic acids. The resultant sample is suitable for virus identification by PCR analysis as well as other nucleic acid manipulations. MSS is designed to disrupt viral particles, denature viral proteins and, therefore, reduce the infection hazard during transport and laboratory processing. NB – BM1677 MSS has been validated only for use with the SARS-CoV-2 virus 2ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Storage: 2-30°C • Store in the dark • Shelf life: 730 days • Samples collected in MSS are suitable for PCR analysis and other nucleic acid manipulations. • MSS is designed to disrupt viral particles, denature viral proteins, inactivate nucleases and reduce the infection hazard during transport and laboratory processing. -
This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium. -
This is a solution of novobiocin used as the selective agent in Modified Semi Solid Rappaport Vassiliadis Medium (BM4031) for the detection of motile Salmonella spp.
-
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar. -
This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
This is a general-purpose neutralising diluent used particularly in the pharmaceutical industry. Lecithin, L-histidine and Tween 80 are present to inactivate surface disinfectants such as quaternary ammonium compounds, phenols, aldehydes (including formaldehyde), hexachlorophene and ethanol. The diluent may be used in sampling surfaces and equipment (including endoscopes) to detect the presence of surviving microorganisms after disinfection. The presence of the surfactant Tween 80 also helps release adherent organisms from surfaces being tested.
-
BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is designed for use with sodium hydroxide (NaOH) treated specimens that have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto the LJ medium after concentration by centrifugation. This medium will isolate most common mycobacteria including M. tuberculosis and can be used for the presumptive identification of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy). BM8200 is recommended by the UK Standards for Microbiology Investigations. NB: This media will not isolate wild strains of M. bovis, which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope such as E&O BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium. -
BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium bovis. BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is designed for use with sodium hydroxide (NaOH) treated samples which have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto LJ media after concentration by centrifugation. This medium differs from E&O BM8200 Non-acidified Glycerol Lowenstein Jensen Medium in that sodium pyruvate has replaced the glycerol, which has been demonstrated to be inhibitory to some species, particularly M. bovis. BM8300 is recommended by the UK Standards for Microbiology Investigations and tested in accordance with the principals of ISO 11133:2014. The medium contains an egg emulsion which provides the required protein and fatty acids. Sodium pyruvate is included as an alternate to glycerol to allow favourable growth of M. bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green and penicillin G are incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 36 ± 1°C for 8 weeks, checking weekly for growth. Further incubation up to 12 weeks may be required for certain Mycobacterium species. -
This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar.
-
For in vitro diagnostic use. BM0540 Nutrient Agar is a basic general-purpose medium suitable for use in the cultivation and subculture of the less fastidious organisms. Nutrient agar was first described by the American Public Health Association (APHA) in 1917. It is used to check the purity of subcultures from isolation plates prior to biochemical or serological tests. It is particularly useful for the cultivation of the less fastidious organisms, particularly those that do not require the addition of blood or other enrichment. It is one of the most important and commonly used non-selective media for the routine cultivation of microorganisms. Nutrient Agar slopes can be used to maintain organisms as it allows prolonged survival of cultures at ambient temperature without the risk of overgrowth that might occur with more nutritious mediums. -
BM0550 Nutrient Broth is a basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms. Nutrient broth is based on the recipe for Nutrient Agar, which was first described by the American Public Health Association (APHA) in 1917 as a medium to cultivate a wide variety of organisms. Although, a basic general-purpose medium, this medium can be enriched with other ingredients such as blood, serum, sodium chloride, sugars, etc., for special purposes.