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Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed primarily as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available. -
Universal Polystyrene Bottle with 30ml volume capacity and a white polystyrene cap Volume(s) : 30ml -
Based on Christensen’s Medium this medium is generally used to detect rapid urease activity of Proteus spp although it can be used to detect urease activity of other Enterobacteriaceae. When used for the later purpose it is necessary to increase the incubation time to as long as 48 hours. -
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. It can also be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. When used for the latter purpose it is necessary to increase the incubation time to as long as 48 hours. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The potassium dihydrogen phosphate is a buffer and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution -
A modification of Christensen’s Medium by Maslen this medium is generally used to detect rapid Urease activity of Proteus spp although it can be used to detect Urease activity of other Enterobacteriaceae including Urease producing Salmonella and Shigella. Unlike Christensen’s Medium when used for the later purpose it is not necessary to increase the incubation time. -
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. Unlike Christensens medium when used for the later purpose it is not necessary to increase the incubation time. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution
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Violet Red Bile Agar is a medium for the enumeration of coliform organisms in food and dairy products and conforms to American Public Health Association (APHA). Yeast extract and enzymatic digest of gelatin provides the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44ºC.
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A medium for the enumeration of coliform organisms in food and dairy products. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non-lactose fermenters produce pale colonies. Selective agents are Bile salts and crystal violet used to inhibit Gram positive and non-enteric organisms. -
Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP. Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.
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Violet Red Bile Glucose Agar Violet Red Bile Glucose Agar is a selective medium for the isolation and enumeration of enterobacteriacae in food products. It is a modification of the original Violet Red Bile Agar (PP1150) with the Lactose being replaced with Glucose. As all members of the enterobacteriacae ferment Glucose it allows for a wider range of organisms to be detected. The medium is made selective by the inclusion of Bile Salts and Crystal Violet to inhibit gram-positive and other non-enteric organisms. -
A modification of Violet Red Bile agar designed to give a ‘coliform’ count. In this medium lactose is substituted with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus V.R.B.G.A gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The growth of non-fermentative Gram negative bacteria can be suppressed by using the agar overlay method. -
The Viral Collection Kit is intended to be used to collect specimens suspected of containing virus and to preserve active virus during transport to a laboratory for analysis (1). The kit consists of a sterile swab for specimen collection from most body areas* and Virus Transport Medium (VTM) for the transportation of viral samples from the patient to the diagnostic laboratory. The stability of viral specimens afforded by the product formulation enables specimen transport and subsequent storage in the 2-30°C range for up to 72 hours before starting analysis. Virus Transport Medium: The inclusion of Hanks balanced salt solution produces an isotonic medium with buffering capacity to maintain pH, provides essential mineral salts, and a visual pH indicator. HEPES provides additional pH stability and bovine serum albumin is present to help stabilise virus particles. Gentamicin and amphotericin B are added to prevent growth of unwanted bacteria and fungi that may be present in the specimen. 1. Clinical and Laboratory Standards Institute (CLSI), 2014. M40-A2 Quality Control of Microbiological Transport Systems; Approved Standard Second Edition. *Not suitable for nasopharyngeal swabbing. -
BM1673 Virus Transport Medium (VTM) is a balanced medium for the transportation of viral samples (with or without a swab). The inclusion of Hanks balanced salt solution produces an isotonic medium with buffer capacity to maintain pH, provide essential mineral salts, and a visual pH indicator. Bovine serum albumin is present to help stabilise virus particles. Gentamicin & amphotericin B are added to prevent growth of bacteria and fungi that may be present in the specimen. • 3ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Non-hazardous • Storage: 2-25°C • Shelf life: 365 days • Diagnostic processing should commence within 72hrs of sample collection. Medium is suitable for analysis via molecular (eg PCR), cell culture and immunofluorescence testing procedures. -
This is a medium for determining the mutagenicity of a chemical reagent using the Ames Test. A histidine requiring strain (His- ) of Salmonella typhimurium is inoculated into a mixture of salt agar, histidine solution and the test reagent, which is mixed and then used to overlay the media. Following incubation if the test reagent is mutagenic it will reverse the His- phenotype allowing growth to occur at a higher level than the control. -
Wort Agar is based on formulations developed by Parfitt (1933) and Scarr (1959), designed to replicate the composition of natural wort. Parfitt focused on yeast and mould counts in butter with Scarr’s later adaptation designed for growth of osmophilic yeast and their detection in sugar products and soft drinks. Wort Agar is primarily used for the cultivation, isolation, and enumeration of yeasts and moulds. It is especially effective for osmophilic yeasts that may be present in butter, sugar products, syrups, soft drinks, and sweet beverages. The low pH and presence of chloramphenicol inhibit bacterial growth. Wort Agar can be used in compliance with ICUMSA (2022) Method GS2-47. -
KM0013 Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation and detection of Salmonella and Shigella spp. in clinical specimens, food, and environmental samples. Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation of Salmonella from food and animal feedstuffs when used according to ISO 6579:2017, ISO 11133:2014. The formulation conforms to CLSI M22 and European, United States and Japanese Pharmacopeia requirements. KM0013 is recommended for clinical specimens as a standard, supplementary or primary isolation medium by the UK Standards for Microbiology Investigations. Xylose Lysine Deoxycholate (XLD) Agar has a pH of 7.4, leaving it with a bright red appearance due to the indicator phenol red. Sugar fermentation lowers the pH and the phenol red indicator registers this by changing to yellow. Most gut bacteria, including Salmonella spp., can rapidly ferment the sugar xylose to produce acid; Shigella spp. cannot do this and therefore remain red. Once the xylose has been used, Salmonella spp. decarboxylate lysine leading to a reversion to an alkaline pH. Additionally, Salmonella spp. (but not Shigella species) are able to reduce thiosulphate to hydrogen sulphide which reacts with ferric ions to produce the black pigment iron sulphide. Salmonella spp. may, therefore, be differentiated since colonies have a black centre on this medium.
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Originally introduced as an aid to recovery of Shigella spp. XLD is also a first class medium for recovery of Salmonella spp. It differs from other media of this type in that it has less Sodium Desoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (Lactose Sucrose and Xylose) together with Lysine Decarboxylase and Sodium Thiosulphate as an indication of the presence or absence of Hydrogen Sulphide. -
Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation and detection of Salmonella and Shigella spp. It differs from other media of this type in that it has less sodium deoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (lactose, sucrose and xylose) together with lysine decarboxylase and sodium thiosulphate as an indication of the presence or absence of hydrogen sulphide. The addition of novobiocin (20mg/L) improves the inhibition of Proteus spp. -
Yeast extract is obtained from selected autolyzed Saccharomyces cerevisiae cells. This product can be used in preparing microbiological culture media providing readily available soluble vitamins (notably B-complexes), amino acids, peptides, and other essential growth factors. Yeast extract is observed as an animal free source and is therefore used extensively in many non-animal cell culture formulations.
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Yeast extract agar is a nutrient rich medium for the cultivation of non-fastidious bacteria, yeasts and moulds. Recommended for the plate count of microorganisms in water and dairy products. The yeast extract and peptone acts as a source of nitrogen, amino acids, carbon and vitamins. Related Supplements : LS0019 Oxytetracycline Selective Supplement
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A nutrient agar corresponding to the standard formulation described in ISO 6222:1999. -
Originally intended for use in surface counting and pour plating techniques in food and dairy products this medium can be used as a general-purpose medium for the cultivation of most non-fastidious organisms. -
This is a selective medium for the isolation and enumeration of yeasts and moulds in dairy products. It is in according to a typical formulation of The International Organisation for Standardisation (ISO). Yeast extract acts as a source of nitrogen, carbon and vitamins in this medium. Glucose is a fermentable carbohydrate. Although the medium has a low pH it is made more selective by the inclusion of chloramphenicol, an antibiotic selective agent.
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This is a selective medium for the isolation and enumeration of yeasts and moulds in dairy products. The medium has a low pH to inhibit bacterial growth and is made more selective by the inclusion of chloramphenicol. It complies with the requirements of ISO 6611 for the enumeration of yeasts and moulds . -
E&O Laboratories Ltd Yersinia (C.I.N.) Selective Supplement (LS1053) is an antibiotic supplement used to enhance the selective isolation of Yersinia spp. in combination with Yersinia C.I.N. Agar. -
In vitro diagnostic. Dehydrated KM0179 Yersinia Selective Agar Base powder can be reconstituted to form a selective plated medium for the isolation and enumeration of Yersinia species from clinical and food samples.
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This is a selective medium for the isolation and enumeration of Yersinia spp. in clinical and food samples. It is made selective by the inclusion of Sodium desoxycholate, Crystal violet and the antimicrobials Cefsulodin, Novobiocin and Irgasan. Mannitol is also included which Yersinia ferments giving a colony that produces a ‘Bull’s Eye’ appearance. The majority of other enteric organisms are inhibited but if they do grow they produce a large pinkish colony with an opaque halo. -
b-Nicotinamide-Adenine Dinucleotide (N.A.D.) Supplement E&O Laboratories Ltd N.A.D. Supplement (LS0005) is an enrichment supplement for Haemophilus spp. and Neisseria gonorrhoeae. This supplement can be used in conjunction with sensitivity test agars such as: E&O products PP2148 Iso Sensitivity test agar with 5% Horse Blood and 20mgs/L N.A.D. and PP0972 Mueller Hinton agar with 5 % Horse Blood with 20mgs/L N.A.D.
