-
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C. NB - It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium. -
This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction. -
A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of two selective agents namely, Chloramphenicol and Nystatin, to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
Trichomonas medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. (1) The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of trichmonads. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The addition of selective agents such as chloramphenicol is recommended to inhibit bacterial species that may be present in specimens. References 1) Kupferberg, A.B. Johnson, G., and Sprince, H. 1948. Proc. Soc. Exper. Biol. Med., 67:304-308 -
Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains. -
Triple sugar iron agar is used to differentiate between some of the enterobacteriacae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide. Beef extract, yeast extract and peptone provide the required nitrogen, carbon and vitamins. Lactose, sucrose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains osmotic balance.
-
This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
-
Triple Vented 140mm Crystal Polystyrene Petri Dish Volume(s) : -
Triple Vented 90mm Crystal Polystyrene Petri Dish Volume(s) : -
Triple Vented 90mm Two Compartment Crystal Polystyrene Petri Dish Volume(s) : -
Tryptone is obtained by pancreatic digestion of casein. Casein is the main protein of milk and is a rich source of amino acid and nitrogen. This product can be used in preparing microbiological culture media providing nitrogen, vitamins, minerals and amino acids. Due to the high tryptophan content in tryptone it can be used in detecting indole production.
-
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli without the need for membranes or pre-incubation. Based on the formulation of Tryptone Bile Agar it incorporates a chromogenic substrate, X-Glucuronide, to detect the ß-glucuronidase enzyme which is specific for the majority of E. coli strains. Approximately, 3-4% of E. coli are glucuronidase negative including E. coli O157.(1) The advantage of the chromogenic substrate is that the reaction is concentrated within the colony resulting in distinctive blue/green colonies of E. coli while the other coliforms produce cream colonies. The tryptone provides the required carbon, nitrogen and vitamins. Bile salts No.3 is a selective agent against Gram-positive bacteria. X-glucuronide is a chromogenic substrate. Agar is solidifying agent.
-
This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
-
This is a plate count agar originally suggested by the American public Health Association for the estimation of total viable counts in food and dairy products. -
This is a general-purpose medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products as well as being suitable in all areas of bacteriological investigation. -
This is a general-purpose medium which supports the growth of a wide range of organisms. It is suitable for Phage Typing, Colicine Typing and for testing the X and V requirements of Haemophilus spp as well as many other areas of bacteriological investigation and conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products. -
Tryptone Soya Agar (TSA) is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. TSA is commonly referred to as Soybean-Casein Digest Agar. TSA supplemented with lecithin and Tween 80® is widely used in environmental monitoring. With further enrichment using 5-10% sheep or horse blood, most fastidious organisms can be isolated and their haemolytic reactions can be determined in order to aid identification. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. The tryptone and soy peptone are the source of the required nitrogen, carbon and vitamins. Sodium chloride maintains the osmotic balance in the medium. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood
-
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP).The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and, in combination with the Lecithin, ethanol and Sodium Thiosulphate inactivates mercurials, halogens and aldehydes). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
-
Tryptone Soya Broth (Modified) with Novobiocin (20mg/L) This is a selective enrichment broth for the isolation of Escherichia coli 0157, primarily from food and food products, and is capable of detecting the organisms even when they are present in small numbers. It is also increasingly being used in clinical laboratories when screening faecal samples. Based on Tryptone Soya Broth it is made selective for Escherichia coli 0157 by the addition of bile salts and Novobiocin and is also buffered to maintain the pH during incubation. This medium is generally used in conjunction with selective agar subculture (e.g. Sorbitol MacConkey Agar with Cefixime Tellurite – (CT-SMAC)). -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
A general purpose broth suitable for the cultivation of most micro-organisms including many fastidious organisms and fungi. It is recommended by the United States Pharmacopoeia for sterility testing of many pharmaceutical products. -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation. -
Tryptone Water is an alternative medium to Peptone Water and more reliable for the testing of Indole production. The medium has a high content of Tryptophan that many organisms, particularly coliforms, break down to form Indole. After incubation add a few drops of Indole reagent to determine the Indole reaction (Red colour is Positive). -
Tryptone yeast extract salts (TYES) agar is a culture medium that can be used as the primary isolation medium for Flavobacterium columnare (1) and F. psychrophilum. Growth of Flavobacterium spp. requires a culture media with a lower nutrient content than that used in general-purpose mediums such as brain heart infusion agar andtryptone soya agar. The tryptone and yeast extract provide the required carbon, nitrogen and vitamins. Calcium chloride and magnesium sulphate provide the required minerals. Agar is a solidifying agent.
-
Tryptose is a mixed enzymatically digested protein. This product can be used in preparing microbiological culture media providing unique nutritional properties useful for cultivating fastidious microorganisms.
-
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed primarily as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available. -
Universal Polystyrene Bottle with 30ml volume capacity and a white polystyrene cap Volume(s) : 30ml -
Based on Christensen’s Medium this medium is generally used to detect rapid urease activity of Proteus spp although it can be used to detect urease activity of other Enterobacteriaceae. When used for the later purpose it is necessary to increase the incubation time to as long as 48 hours. -
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. It can also be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. When used for the latter purpose it is necessary to increase the incubation time to as long as 48 hours. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The potassium dihydrogen phosphate is a buffer and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution
-
A modification of Christensen’s Medium by Maslen this medium is generally used to detect rapid Urease activity of Proteus spp although it can be used to detect Urease activity of other Enterobacteriaceae including Urease producing Salmonella and Shigella. Unlike Christensen’s Medium when used for the later purpose it is not necessary to increase the incubation time. -
A modification of Christensens medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. Unlike Christensens medium when used for the later purpose it is not necessary to increase the incubation time. The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator. Related Supplements : BM3000 Urea 40% Solution
-
Violet Red Bile Agar is a medium for the enumeration of coliform organisms in food and dairy products and conforms to American Public Health Association (APHA). Yeast extract and enzymatic digest of gelatin provides the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44ºC.
-
A medium for the enumeration of coliform organisms in food and dairy products. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same colour. Non-lactose fermenters produce pale colonies. Selective agents are Bile salts and crystal violet used to inhibit Gram positive and non-enteric organisms. -
Violet Red Bile Glucose Agar (VRBGA) is a selective medium for the isolation and enumeration of Enterobacteriaceae in food products. It is a modification of the original Violet Red Bile Agar with the lactose being replaced with glucose. As all Enterobacteriaceae ferment glucose VRBGA allows for a wider range of organisms to be detected. This medium conforms to the requirements of the Harmonised USP/EP/JP. Yeast extract and pancreatic digest of gelatin provide the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium chloride maintains the osmotic balance. The medium is made selective by the inclusion of bile salts and crystal violet to inhibit Gram-positive and non-enteric organisms.
-
Violet Red Bile Glucose Agar Violet Red Bile Glucose Agar is a selective medium for the isolation and enumeration of enterobacteriacae in food products. It is a modification of the original Violet Red Bile Agar (PP1150) with the Lactose being replaced with Glucose. As all members of the enterobacteriacae ferment Glucose it allows for a wider range of organisms to be detected. The medium is made selective by the inclusion of Bile Salts and Crystal Violet to inhibit gram-positive and other non-enteric organisms. -
A modification of Violet Red Bile agar designed to give a ‘coliform’ count. In this medium lactose is substituted with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus V.R.B.G.A gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The growth of non-fermentative Gram negative bacteria can be suppressed by using the agar overlay method. -
The Viral Collection Kit is intended to be used to collect specimens suspected of containing virus and to preserve active virus during transport to a laboratory for analysis (1). The kit consists of a sterile swab for specimen collection from most body areas* and Virus Transport Medium (VTM) for the transportation of viral samples from the patient to the diagnostic laboratory. The stability of viral specimens afforded by the product formulation enables specimen transport and subsequent storage in the 2-30°C range for up to 72 hours before starting analysis. Virus Transport Medium: The inclusion of Hanks balanced salt solution produces an isotonic medium with buffering capacity to maintain pH, provides essential mineral salts, and a visual pH indicator. HEPES provides additional pH stability and bovine serum albumin is present to help stabilise virus particles. Gentamicin and amphotericin B are added to prevent growth of unwanted bacteria and fungi that may be present in the specimen. 1. Clinical and Laboratory Standards Institute (CLSI), 2014. M40-A2 Quality Control of Microbiological Transport Systems; Approved Standard Second Edition. *Not suitable for nasopharyngeal swabbing. -
BM1673 Virus Transport Medium (VTM) is a balanced medium for the transportation of viral samples (with or without a swab). The inclusion of Hanks balanced salt solution produces an isotonic medium with buffer capacity to maintain pH, provide essential mineral salts, and a visual pH indicator. Bovine serum albumin is present to help stabilise virus particles. Gentamicin & amphotericin B are added to prevent growth of bacteria and fungi that may be present in the specimen. • 3ml in self-standing M043 Tube • Aseptically filled • Red cap with swab capture point • Non-hazardous • Storage: 2-25°C • Shelf life: 365 days • Diagnostic processing should commence within 72hrs of sample collection. Medium is suitable for analysis via molecular (eg PCR), cell culture and immunofluorescence testing procedures. -
This is a medium for determining the mutagenicity of a chemical reagent using the Ames Test. A histidine requiring strain (His- ) of Salmonella typhimurium is inoculated into a mixture of salt agar, histidine solution and the test reagent, which is mixed and then used to overlay the media. Following incubation if the test reagent is mutagenic it will reverse the His- phenotype allowing growth to occur at a higher level than the control. -
Originally introduced as an aid to recovery of Shigella spp. XLD is also a first class medium for recovery of Salmonella spp. It differs from other media of this type in that it has less Sodium Desoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (Lactose Sucrose and Xylose) together with Lysine Decarboxylase and Sodium Thiosulphate as an indication of the presence or absence of Hydrogen Sulphide. -
Xylose Lysine Deoxycholate (XLD) Agar is used for the isolation and detection of Salmonella and Shigella spp. It differs from other media of this type in that it has less sodium deoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (lactose, sucrose and xylose) together with lysine decarboxylase and sodium thiosulphate as an indication of the presence or absence of hydrogen sulphide. The addition of novobiocin (20mg/L) improves the inhibition of Proteus spp. -
Xylose Lysine Deoxycholate (XLD) agar is used for the isolation and detection of Salmonella and Shigella spp. Developed by Taylor, xylose lysine agar base was used for isolating and differentiating Gram-negative enteric bacilli. The addition of sodium thiosulfate, ferric ammonium citrate, and sodium deoxycholate created the more selective medium, XLD agar. This medium was found to be satisfactory for the isolation of Shigella and Providencia spp., as well as proving to be an effective differential media. The yeast extract is the source of the required nitrogen, carbon and vitamins. Lactose, sucrose and xylose are fermentable carbohydrates. Sodium deoxycholate, sodium thiosulphate and ferric ammonium citrate are selective agents. Phenol red acts as a pH indicator. Sodium chloride maintains the osmotic balance. Most enteric bacteria including Salmonella spp., can ferment xylose to produce acid. Shigella spp. are unable to do this and thus the colonies remain red. Once xylose has been completely utilized Salmonella spp. will decarboxylate lysine resulting in a pH increase to alkaline. Salmonella and Shigella spp. are differentiated as Salmonellae spp. are able to metabolise thiosulphate producing hydrogen sulphide resulting in colonies with black centres. Stool specimens or rectal swabs may be plated directly onto XLD agar. Selective enrichment broths, such as Selenite Broth or Tetrathionate Broth, may be used prior to streaking. For specific procedures refer to appropriate references.
