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Sodium Hydroxide (4%) with Phenol Red is a reagent used in the digestion and decontamination of sputum samples prior to culture for mycobacteria. Most clinical specimens submitted for acid-fast bacilli isolation are contaminated with more quickly developing commensal microbial flora. Additionally, acid-fast bacilli may be retained in respiratory secretions and not released for culture until the material is liquefied. Respiratory specimens, such as sputum, contain mucin which may trap microorganisms. Decontamination and digestion of the mucous components kills contaminating normal flora and allows slower growing mycobacteria to grow. Timely neutralization prevents potential loss of mycobacteria caused by high pH levels of decontaminants, resulting in the preservation of more viable organisms. Phenol red is used to indicate changes in pH. BM1320 is recommended by the UK Standards for Microbiology Investigations as part of their decontamination/digestion protocol; it is generally used in conjunction with Sputum Neutralising Buffer without Phenol Red (BM1324). -
This is a differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey media in that lactose has been replaced by sorbitol. As Escherichia coli 0157:H7 does not ferment sorbitol, it produces pale translucent colonies whereas most other strains of Escherichia coli do ferment sorbitol and produce pink colonies. -
Sorbitol MacConkey agar is a differential medium for the isolation of Escherichia coli 0157:H7 based on the formulation by Rappaport and Henig. It differs from other MacConkey mediums in that lactose has been replaced by sorbitol. As Escherichia coli 0157:H7 does not ferment sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are sorbitol positive and produce pink colonies. Although it should be noted that colonies that are sorbitol positive can revert and possibly be mistaken as sorbitol negative. Tryptone and meat peptone provide the required carbon, nitrogen and vitamins. Sorbitol is a fermentable carbohydrate and neutral red is a pH indicator. Bile Salts no.3 and crystal violet are selective agents and together inhibit Gram-positive cocci. Sodium chloride maintains the osmotic balance. If required, the selectivity of the medium may be increased by the addition of cefixime (0.05mg/L) and potassium tellurite (2.5mg/L). Related Supplements : LS0013 Escherichia coli 0157 Selective Supplement -
Sorbitol MacConkey with Cefixime & Tellurite (CT-Smac) This is a selective differential medium for the isolation of Escherichia coli O157:H7. It differs from other MacConkey medium in that Lactose has been replaced by Sorbitol. As Escherichia coli O157:H7 does not ferment Sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are Sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial Cefixime and Potassium Tellurite. -
Soy peptone is manufactured from the enzymatic hydrolysis of soybean. This product provides a good source of nitrogen, carbohydrates, and vitamins. It is recommended for use in microbiological media for the detection and isolation of a wide variety of bacteria and fungi.
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This is a sterile aqueous solution of Potassium di-hydrogen phosphate (16.0 % w/v) with Phenol Red Indicator suitable for use in the neutralisation following the digestion of sputum with Sodium hydroxide prior to culture. It is generally used in conjunction with BM1322 Sodium hydroxide 4%. -
This is a sterile aqueous solution of Potassium Di-Hydrogen Ortho-phosphate (16.0 % w/v) suitable for use in the neutralisation following the digestion of Sputum with Sodium Hydroxide prior to culture. It is generally used in conjunction with Sodium Hydroxide 4% with Phenol Red Indicator 0.2%. -
Staph/Strep Selective Supplement E&O Laboratories Ltd Staph/Strep Selective Supplement (LS0008) is an antibiotic supplement used to enhance the selective isolation of Staphylococci and Streptococci species. -
E&O Heat Inactivated Serum is derived from horse serum and is suitable for use in diagnostic assays. One of the reasons for the heat inactivation of serum (heating to 56°C for 30 min) is to inactivate complement, a group of proteins present in serum that are part of the immune response. This is sometimes important for cells that will be used to prepare or assay viruses, used in cytotoxicity assays or other systems where complement may have an unwanted influence. The use of Heat Inactivated Serum is also usually recommended for growing embryonic stem cells. After filtration the dispensing and bottle filling processes are carried out in a state-of-the-art clean room under laminar flow. Once labelled the filled bottles are then subjected to controlled heat inactivation and are frozen and stored at -20°C without delay. The filter sterile Heat Inactivated Horse Serum is supplied in 100 or 500 ml PETG bottles. All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
All E&O products are for in vitro use only. E&O products are intended only for use by qualified professionals who will safely handle and dispose of products they receive. All biologically derived materials (e.g. blood, sera) should be handled as if a potential biohazard. E&O media that contain antibiotics should be handled with care. Chemical resistant gloves, eye protection and laboratory coat should be worn. -
This medium is utilised for the transportation and cryopreservation of Streptococcus pneumoniae & Neisseria meningitidis isolates. -
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp particularly Mycobacterium bovis. This medium is used primarily in the veterinarian sector. The medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains pyruvate and egg which provide the required protein and fatty acids. It differs from Lowenstein-Jensen Medium in that Sodium Pyruvate has replaced the Glycerol, which has been demonstrated to be inhibitory to some species, particularly M.bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium. -
This product may be used as an alternative pre-treatment solution to lessen the background flora of samples before the testing for Mycobacteria species. This reagent should only be used for samples that routinely produce contaminated cultures after processing with an alkaline digestant. -
This product may be used as a pre treatment solution to lessen the background commensal flora of samples before the testing for Mycobacteria species. -
This buffer is intended primarily for use as a neutralising agent following treatment with alkaline compounds during the decontamination and homogenisation process of Sputum specimens prior to inoculation onto appropriate culture media for the isolation of Mycobacterium spp. -
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C. NB - It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium. -
This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction. -
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical and food samples. The formulation was developed by Kobayashi et al. which was modified from Nakanishi’s formulation. Vibrio species are most widely recognized for their role in human intestinal infections and cholera worldwide. Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar is recommended by the FDA BAM, the UK Standards for Microbiology Investigations, and complies with the standard laid out by ISO 21872.
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A nutritious broth medium formulated by Todd and Hewitt for the production of antigenic streptococcal haemolysin. The broth is also used to cultivate streptococci prior to serological grouping. Normally the use of fermentable sugars in the broth would inactivate the haemolysin due to acid production. This is prevented by the use of buffers to maintain the pH in this broth. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
Trichomonas Broth (CPLM) with Nystatin is for the selective isolation of Trichomonas spp. This medium is based on the formula described by Kupferburg et al.. The superiority of the culture procedure over the wet mount procedure for detecting the presence of trichomonads in clinical specimens was demonstrated by Williams, and Kean and Day. Feinberg and Whittington demonstrated the greater accuracy of the culture procedure for detecting trichomonads in clinical material. The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of Trichomonas spp.. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The medium is selective due to the inclusion of chloramphenicol and nystatin to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. Cultures may be examined microscopically after 48 hours incubation at 37 ± 1°C for the presence of flagellate protozoans. If a negative result is obtained, then the culture may be re-incubated for a further 72 hours.
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Trichomonas medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. (1) The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate. The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of trichmonads. Methylene blue is a redox indictor and allows for the visualisation of any significant oxygen diffusion in the medium. The addition of selective agents such as chloramphenicol is recommended to inhibit bacterial species that may be present in specimens. References 1) Kupferberg, A.B. Johnson, G., and Sprince, H. 1948. Proc. Soc. Exper. Biol. Med., 67:304-308
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Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains. -
Triple sugar iron agar is used to differentiate between some of the enterobacteriacae on the basis of four reactions: fermentation of lactose, glucose and sucrose and the production of hydrogen sulphide. Beef extract, yeast extract and peptone provide the required nitrogen, carbon and vitamins. Lactose, sucrose and glucose are carbohydrates. Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains osmotic balance.
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This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
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Triple Vented 140mm Crystal Polystyrene Petri Dish Volume(s) : -
Triple Vented 90mm Crystal Polystyrene Petri Dish Volume(s) : -
Triple Vented 90mm Two Compartment Crystal Polystyrene Petri Dish Volume(s) : -
Tryptone is obtained by pancreatic digestion of casein. Casein is the main protein of milk and is a rich source of amino acid and nitrogen. This product can be used in preparing microbiological culture media providing nitrogen, vitamins, minerals and amino acids. Due to the high tryptophan content in tryptone it can be used in detecting indole production.
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Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli without the need for membranes or pre-incubation. Based on the formulation of Tryptone Bile Agar it incorporates a chromogenic substrate, X-Glucuronide, to detect the ß-glucuronidase enzyme which is specific for the majority of E. coli strains. Approximately, 3-4% of E. coli are glucuronidase negative including E. coli O157.(1) The advantage of the chromogenic substrate is that the reaction is concentrated within the colony resulting in distinctive blue/green colonies of E. coli while the other coliforms produce cream colonies. The tryptone provides the required carbon, nitrogen and vitamins. Bile salts No.3 is a selective agent against Gram-positive bacteria. X-glucuronide is a chromogenic substrate. Agar is solidifying agent.
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This is a plate count agar originally suggested by the American Public Health Association for the estimation of total viable counts in food and dairy products.
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This is a plate count agar originally suggested by the American public Health Association for the estimation of total viable counts in food and dairy products. -
This is a general-purpose medium supporting a wide range of organisms. It conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products as well as being suitable in all areas of bacteriological investigation. -
This is a general-purpose medium which supports the growth of a wide range of organisms. It is suitable for Phage Typing, Colicine Typing and for testing the X and V requirements of Haemophilus spp as well as many other areas of bacteriological investigation and conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products. -
Tryptone Soya Agar is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or simply general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. Tryptone Soya Agar may be used to determine X and V factor requirements of Haemophilus species; sterility testing; and environmental monitoring within pharmaceutical cleanrooms and sterile facilities. This medium meets the requirements of the Harmonized USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. Tryptone Soya Agar is recommended as a reference medium when testing selective media, to measure the degree of inhibition. In environmental monitoring applications, it is common for plates to be incubated at 30-35°C for bacterial colonies and 20-25°C for mould and fungi. Tryptone Soya Agar will support the growth of both aerobic and anaerobic organisms depending on incubation conditions. KM0024 unsupplemented is recommended by the World Health Organization, UK Standards for Microbiology Investigations, International Organization for Standardization and is tested in accordance with ISO 11133:2014. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing. UK Standards for Microbiology Investigations also call for Tryptone Soya Agar supplemented with 5% sheep blood (E&O DSC) for aid in the identification of Bordetella species from clinical specimens. The addition of defibrinated animal blood to the base medium, promotes the growth of most fastidious organisms and presumptive identification can be made based on haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood. Addition of selective agents allows isolation and presumptive identification of specific species or groups of organisms. Related Supplements : Defibrinated Sheep Blood, Defibrinated Horse Blood
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This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP).The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
BM1436 Tryptone Soya Agar (pH 7.2) is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. This medium meets the requirements of The British Standards Institution (1) and is tested according to the principles of ISO 11133:2014 (2). and is based on the original formulation described by Leavitt et al. in 1955 (3). In environmental monitoring applications, it is common for plates to be incubated at 30-35°C for bacterial colonies and 20-25°C for mould and fungi (4).
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
- International Organization for Standardization (2014) 11133:2014 Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media. Geneva, ISO.
- Leavitt, J., Naidorf, I. and Shugaevsky, P. (1955) Aerobes and anaerobes in endodontics. Part I. The undetected anaerobes in endodontics. Part II. Sensitive culture medium for the detection of both aerobes and anaerobes. NYSDJ, 25, pp.377-382.
4. Anon. (1987) Testing methods for use in quality assurance of culture media. Int. J. Food Microbiol., 5, pp.291-296
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This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). The medium can be incubated under aerobic or anaerobic conditions after use for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). Lecithin and Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP). Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and, in combination with the Lecithin, ethanol and Sodium Thiosulphate inactivates mercurials, halogens and aldehydes). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
A general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and soy peptone are the nitrogen and vitamin source in the medium. Glucose is the carbon energy source that facilitates organism growth and sodium chloride maintains osmotic balance. Di-potassium hydrogen phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. TSB conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
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Tryptone Soya Broth (Modified) with Novobiocin (20mg/L) This is a selective enrichment broth for the isolation of Escherichia coli 0157, primarily from food and food products, and is capable of detecting the organisms even when they are present in small numbers. It is also increasingly being used in clinical laboratories when screening faecal samples. Based on Tryptone Soya Broth it is made selective for Escherichia coli 0157 by the addition of bile salts and Novobiocin and is also buffered to maintain the pH during incubation. This medium is generally used in conjunction with selective agar subculture (e.g. Sorbitol MacConkey Agar with Cefixime Tellurite – (CT-SMAC)). -
Tryptone Soya Broth is also commonly referred to as Soybean-Casein Digest Medium or Tryptic Soya Broth and is abbreviated as TSB. This is a general purpose and nutritious medium for the non-selective enrichment of non-fastidious and some fastidious organisms. Tryptone and Soy peptone are the nitrogen sources in the medium. Glucose is the carbon energy source that facilitates organism growth. Sodium chloride maintains osmotic balance; Di-potassium phosphate is a buffering agent to prevent auto-sterilisation due to acid production during the growth of some organisms. Tryptone Soya Broth formulation conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). -
A general purpose broth suitable for the cultivation of most micro-organisms including many fastidious organisms and fungi. It is recommended by the United States Pharmacopoeia for sterility testing of many pharmaceutical products. -
This is a general-purpose broth for cultivation of fastidious bacteria, yeasts and moulds. The medium incorporates Polysorbate 80 to act as an emulsifying agent and inactivate phenols. Lecithin is also incorporated to inactivate quaternary ammonium compounds. The medium can be incubated under aerobic or anaerobic conditions for sterility testing, and other areas of bacteriological investigation. -
Tryptone Water is an alternative medium to Peptone Water and more reliable for the testing of Indole production. The medium has a high content of Tryptophan that many organisms, particularly coliforms, break down to form Indole. After incubation add a few drops of Indole reagent to determine the Indole reaction (Red colour is Positive). -
Tryptone yeast extract salts (TYES) agar is a culture medium that can be used as the primary isolation medium for Flavobacterium columnare (1) and F. psychrophilum. Growth of Flavobacterium spp. requires a culture media with a lower nutrient content than that used in general-purpose mediums such as brain heart infusion agar andtryptone soya agar. The tryptone and yeast extract provide the required carbon, nitrogen and vitamins. Calcium chloride and magnesium sulphate provide the required minerals. Agar is a solidifying agent.
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Tryptose is a mixed enzymatically digested protein. This product can be used in preparing microbiological culture media providing unique nutritional properties useful for cultivating fastidious microorganisms.
