-
Columbia Agar Base with 7% Horse Blood & 50mg/L Neomycin Based on Columbia Agar enriched with 7% Horse Blood this formulation has been modified to include Neomycin, which will inhibit most gram-positive and gram-negative aerobes, making it suitable for use as a selective medium for the isolation of many anaerobes. -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 7% Horse Blood, suitable for the cultivation and maintenance of most organisms including many fastidious anaerobes of clinical significance. -
A very nutritious general-purpose medium based on Columbia Agar Base enriched with 7% Sheep Blood, suitable for the isolation of most organisms including most fastidious anaerobes of clinical significance. Many workers claim that β haemolysis is more readily apparent, particularly in group A streptococci, when Sheep Blood is used in place of Horse Blood. -
This is a selective medium for the isolation of Staphylococcus and Streptococcus spp. Based on Columbia Agar, it is enriched by the addition of Sheep Blood (7%) the medium is also made selective by the inclusion of Colistin and Naladixic Acid to suppress the growth of the majority of Gram negative bacteria. The addition of sheep blood the medium allows for good colonial appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. -
Columbia Blood Agar with 5% Defibrinated Horse Blood & Cap Selective Supplement This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 5% Horse Blood the medium is made selective by the inclusion of Colistin and Aztreonam to suppress the growth of the majority of Gram negative bacteria. -
This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 7% Horse Blood the medium is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of Gram negative bacteria. -
A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 60°C. Suitable for the cultivation of most pathogens including many fastidious organisms and is particularly suitable for Haemophilus and Neisseria spp. -
BM0110 Cooked Meat with Brain Heart Infusion Broth Overlay is suitable for recovery, isolation, and storage of the most fastidious anaerobes, such as Clostridium species as well as many aerobes. Cooked Meat Medium prepared from heart tissue is a well-established medium for the cultivation of anaerobic and aerobic organisms. Cooked Meat with Brain Heart Infusion Broth Overlay has been shown to initiate bacterial growth from very small innocua and to maintain the viability of cultures over long periods of time; it is especially useful in culturing from blood samples. Mixed cultures of bacteria survive without displacing the slower growing organisms. The products of growth do not rapidly destroy the inoculated organisms and therefore it is an excellent medium for the storage of aerobic and anaerobic bacteria. -
Clear soda lime glass culture tube with screw neck and 13mm gold metal cap. Dimensions: 100mm x 16mm Capacity(s) : ml -
This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of Nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of yeasts. It has also been used for the identification of Candida albicans by chlamydospore production. -
Dey & Engley Broth has the ability to neutralise antimicrobial chemicals and is used for environmental sampling for the detection and enumeration of microorganisms present in cosmetic and/or environmental samples. In this formulation the broth has been modified by the addition of Tween 80 (Polysorbate 80), Lecithin, Sodium thioglycollate, Sodium thiosulphate and Sodium bisulphite. It is recommended for use in environmental testing, particularly in areas subjected to surface disinfection, and conforms to the requirements of the British Pharmacopeia for sterility testing of appropriate pharmaceutical products. The product also conforms to ISO 21149 to act as a diluent in the method for the enumeration of aerobic bacteria from cosmetic samples. Lecithin and Polysorbate 80 (Tween 80) inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). Sodium thioglycollate neutralises mercurials, Sodium thiosulphate neutralises iodine and chlorine and Sodium bisulphite neutralises aldehydes. Bromocresol purple is incorporated into the medium to indicate glucose utilisation. -
This medium is used for the cultivation and enumeration of Lactobacillus spp. MRS agar is based on the formulation by de Man, Rogosa and Sharpe. The peptone, yeast extract and beef extract provides the reuired carbon, nitrogen and vitamin source. Glucose is a fermentable carbohydrate. The magnesium sulphate and manganese sulphate act as growth stimulants. Potassium phosphate is a buffering agent. Selectivity of the medium is achieved through the use of ammonium citrate and sodium acetate, inhibiting microorganisms such as streptococci and moulds. The addition of the surfactant Tween 80 is required to facilitate the uptake of nutrients from Lactobacillus spp. -
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated horse blood is aseptically collected whole horse blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of horse blood are not identical to sheep blood and blood agar media designed for horse blood may not be satisfactory with sheep blood and vice versa. -
It is not possible to sterilise whole blood products and therefore they must be collected aseptically. Horse and sheep blood are the most widely used animal blood products in culture media. The choice of which type of blood to use with culture media is largely traditional, with much of continental Europe preferring sheep blood, whilst the UK and certain parts of the Commonwealth prefer horse blood. Defibrinated sheep cells are aseptically collected whole sheep blood that has been processed to remove fibrin. There are no additives or preservatives in this product. Defibrination is now accepted as the best method of preventing blood clotting. It must be carried out immediately after drawing the blood and the agitation must be sufficient to denature the fibrinogen but not to cause rupture of the erythrocytes and haemolysis. The haemolytic reactions of sheep blood are not identical to the reactions of horse blood and blood agar media designed for sheep blood may not be satisfactory with horse blood and vice versa. -
For in vitro diagnostic use. BM0650 Deionised Water is used as a diluent for, where required, the preparation of clinical specimens and microbiological samples, the dilution of microorganisms and reconstitution of lyophilised antibiotic supplements during culture media preparation. Deionised Water is purified water that can be used as a general-purpose diluent in many areas of the laboratory. It may be used for creating 0.5 McFarland suspensions of bacterial isolates for identification tests or processing clinical specimens or industrial samples prior to microbiological examination. -
This is a selective medium for the isolation of Salmonella spp. and Shigella spp. from clinical specimens and environmental samples. This formulation was developed by Hynes through a modification of Leifson’s DCA medium. The peptone provides the required carbon, nitrogen and vitamins. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium thiosulphate and ferric citrate are used to detect hydrogen sulphide production indicated by the black centred colonies of hydrogen sulphite positive organisms. Sodium deoxycholate and sodium citrate inhibit most Gram-positive organisms.
-
A selective medium for the isolation and detection of dermatophytic fungi originally developed by Taplin et al. Dermatophytes appear as fluffy colonies with reddening of the medium while other fungi cause the medium to become yellow due to acid production. Yeasts will also grow on this medium but are readily distinguished by their distinct white/creamy colonies and distinctive smell. Soy peptone provides the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. The inclusion of phenol red assists in the differentiation between saprophytic and environmental fungi. Although the low pH (pH 5.5) of the medium inhibits most bacteria chloramphenicol and cycloheximide are often added to further reduce the risk when processing material that may be more heavily contaminated. NB: Prolonged incubation should be avoided as this may cause fungi other than dermatophytes to turn the medium red. Related Supplements : LS0050 Chloramphenicol Selective Supplement
-
Dermatophyte Test Medium with Chloramphenicol & Cyclohexamide (Actidione) This is a selective medium for the isolation of dermatophytes that includes a Phenol Red indicator to assist in the differentiation between dermatophytes and other pathogenic fungi. Although the low pH (5.5) of the medium inhibits most bacteria, Chloramphenicol is added to further reduce the risk when processing material that may be more heavily contaminated. Cyclohexamide (Actidione) is also added to suppress the growth of most yeasts and saprophytic fungi. Dermatophytes appear as fluffy white colonies and produce a red colour on the medium while other fungi cause the medium to become yellow due to acid production. Yeasts will also grow on this medium but are readily distinguished by their distinct white/creamy colonies and distinctive smell. NB: Prolonged incubation should be avoided as this may cause fungi other than dermatophytes to turn the medium red -
Desoxycholate Citrate Agar (DCA) (Hynes) One of several media designed for the differential selective isolation of Salmonella and Shigella spp from clinical and environmental samples. Lactose is added to the medium together with Neutral Red indicator to assist in the differentiation of Lactose and Non-Lactose fermenting organisms. The medium is made selective by the inclusion of Sodium Desoxycholate and Sodium Thiosulphate, which will inhibit most gram-positive organisms. The Sodium Thiosulphate is also broken down by the enzyme thiosulphate reductase to form Sodium Sulphite and Hydrogen Sulphide. The Hydrogen Sulphide, if present, will in turn react with the ferric ions to produce a black precipitate of Ferrous Sulphide and give rise to the classical black centre of most Salmonellae. It has been suggested that this medium may be more suitable for secondary post-enrichment isolation while the original formulation is more appropriate for the primary inoculation of samples. -
Dichloran Rose-Bengal Chloramphenicol (DRBC) Agar Dichloran Rose Bengal Chloramphenicol Agar is based on the formulation described by King et al. It is a selective medium for the isolation and enumeration of yeasts and mould that are of significance in food spoilage. The medium is a modification to Rose Bengal Chloramphenicol Agar and is recommended by the International Standard ISO 21527:2008 part 1. -
Dichloran Rose Bengal Chloramphenicol (DRBC) agar is based on the formulation described by King et al. and is used for the selective isolation and enumeration of yeasts and moulds from food samples. The peptone provides the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. Potassium di-hydrogen phosphate is a buffering agent and magnesium sulfate is a source of divalent ions and sulfate. In order to curtail the size of the colony diameters of spreading fungi, the antifungal agent dichloran is added to the base and the pH is reduced to 5.6. Rose Bengal suppresses growth of bacteria and restricts the size and height of colonies of more rapidly growing molds. The inclusion of chloramphenicol ensures the suppression of bacteria present in environmental and food samples. Rose Bengal is absorbed by yeast and mold colonies and this further aids in their enumeration. Occasionally reduced recovery of yeasts may be encountered due to the increased activity of Rose Bengal at pH 5.6.
-
For in vitro diagnostic use. BM0655 Distilled Water can be used in the preparation of bacterial suspensions and as a diluent in areas of the laboratory. Distilled Water is manufactured from the distillation of deionised water. During this process almost all the impurities in the water are removed and this is why distilled water is chosen for specific laboratory applications. -
DN'ase Medium DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out. -
DNase agar is used primarily in clinical laboratories to differentiate pathogenic Staphylococcus aureus from other staphylococci based on deoxyribonuclease (DNase) activity. The tryptone provides the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. The addition of DNA to the base medium provides a simple method to check for DNase activity. Following incubation of the inoculated medium, the surface of the medium is flooded with a small quantity of 1M hydrochloric acid to precipitate the DNA. This results in the medium turning opaque. Organisms that can produce sufficient quantity of a DNase enzyme will hydrolyse the DNA resulting in a clear area around the colonies. Whereas DNase negative organisms will not produce clearing. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. coagulase test, latex agglutination etc., should be carried out.
-
DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other staphylococci based on deoxyribonuclease activity. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a colourless zone around the colonies, described as being DNase positive, whereas coagulase negative staphylococci do not produce clearing. This particular formulation can also be used for Streptococci and Serratia. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out. -
DNase agar is used primarily in clinical laboratories to differentiate pathogenic Staphylococcus aureus from other staphylococci based on deoxyribonuclease (DNase) activity. The tryptone provides the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. The addition of DNA to the base medium provides a simple method to check for DNase activity. The methyl green fades into a colourless compound if the DNA in the medium is depolymerised. Organisms that can produce sufficient quantity of a DNase enzyme will hydrolyse the DNA resulting in a clear area around the colonies. Whereas DNase negative organisms will not produce clearing. NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. coagulase test, latex agglutination etc., should be carried out.
-
Legionellae are more resistant to lower pH and brief exposure to higher temperatures than many other freshwater bacteria. For some water samples with high concentrations of bacteria, it is necessary to use a selective procedure to reduce the number of non-Legionella bacteria before culture. Acid treatment and heating of samples are routinely used for this purpose and to aid antigen extraction. The use of this buffer is recommended in the International Standard ISO 11731-2 "Water quality-Detection and enumeration of Legionella" for this purpose. -
Durham Tubes available in 6x30mm and 8x35mm Volume(s) : N/A -
E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non-lactose fermenting organisms. This formulation complies with the Harmonized USP/EP/JP. -
Edwards Medium (Modified) with 7% Sheep Blood This is a medium for the selective isolation of streptococci, particularly Streptococcus agalactiae, involved in bovine mastitis. The medium is enriched by the addition of 7% Sheep Blood and made selective by the inclusion of Crystal Violet and Thallous Sulphate. Aesculin is also present and assists in the differentiation of Streptococcus agalactiae, which give rise to blue colonies, from Aesculin positive Group D streptococci which produce black colonies. -
A sterile concentrated emulsion of premium egg yolks, suitable for incorporation in culture media which detect lecithinase production by bacteria. It can be used in media for Bacillus cereus and Staphylococci. Used with serum and Filde’s extract it may be used to produce Nagler plates for Clostridia. -
This is a sterile emulsion of Egg Yolk in Saline containing Potassium Tellurite and is generally used as a selective differential agent in Baird Parker Medium. The complete medium is selective for Staphylococcus aureus as the Potassium Tellurite inhibits most coliform organisms and is also reduced by Staphylococcus aureus to tellurite giving typical black colonies on the Baird Parker Medium. -
Enterobacteriaceae Enrichment Broth E.E. Broth is recommended as an enrichment medium when examining food and feedstuffs for Enterobacteriaceae. It is a modification of Brilliant Green Bile Broth, with an improved buffering capacity to encourage early growth and prevent autosterilization. E.E. Broth uses glucose instead of lactose to make the medium a test for all enterobacteria including non lactose fermenting organisms. -
Eosin Methylene Blue agar (EMB) is a selective medium primarily for the isolation of coliforms from clinical, food and environmental samples. This is the modified formulation of EMB proposed by Levine with a higher concentration of lactose and the sucrose omitted. The peptone provides the required carbon, nitrogen and vitamins. Lactose is a fermentable carbohydrate and di-potassium phosphate is a buffer. Eosin Y and methylene blue are indicators. Methylene blue is also a selective agent. During strong acidic conditions, the dyes impart a metallic sheen to certain lactose fermenters, such as Escherichia coli.
-
Escherichia coli 0157 Selective Supplement E&O Laboratories Ltd Escherichia coli 0157 Selective Supplement (LS0013) is an antibiotic supplement used to enhance the isolation of Escherichia coli serogroup O157 from faecal, food and environmental specimens. -
KM0006 Fastidious Anaerobe Agar is a general-purpose medium, when supplemented with blood and any necessary selective agents, can be used for the isolation and culture of fastidious anaerobes from clinical specimens. Anaerobic infections are common infections caused by anaerobic bacteria. These bacteria occur naturally and are the most common flora in the body; in their natural state, they do not cause infection. Anaerobic bacteria can infect deep wounds, tissue, and internal organs where there is little oxygen after injury or surgery. Infection by anaerobic bacteria is normally characterized by formation of abscesses, foul smelling discharge, pus, and free gas in tissue, leading to tissue degradation. KM0006 is recommended as a standard, primary isolation or supplementary medium by the UK Standards for Microbiology Investigations and tested in accordance with the principles of ISO 11133:2014. Related Supplements : LS0015 Actinomycete Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficile Selective Supplement, LS0023 Clostridium perfringens Selective Supplement, BM0140 Egg Yolk Emulsion
-
Fastidious Anaerobe Agar (FAA) with 5% Sheep Blood Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (Starch & Sodium Bicarbonate) growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. In this instance the medium is further enriched by the addition of 5% Sheep Blood. -
Fastidious Anaerobe Agar (FAA) with 7% Horse Blood & Neomycin (75mg/L) Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (Starch & Sodium Bicarbonate) as well as growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. In this instance the medium is made more selective by the inclusion of 75mg/L of Neomycin to inhibit most enteric organisms and further enriched by the addition of 7% Horse Blood. -
Fastidious Anaerobe Agar with 7% Horse Blood, Vancomycin (6mg/L) & Nalidixic Acid (10mg/L) This is a selective medium for the isolation of gram-negative anaerobes from clinical specimens. The base medium, Fastidious Anaerobe Agar, is complex and includes detoxification agents (Starch & Sodium Bicarbonate), growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production where appropriate. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. The medium is made selective, by the inclusion of Naladixic Acid and Vancomycin and further enriched by the addition of 7% Horse Blood. -
Fastidious Anaerobe Agar with 7% Horse Blood Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes including fastidious organisms. The formulation is complex and includes detoxification agents (Starch & Sodium Bicarbonate) growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production in Porphyromonas melaninogenicus. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. In this instance the medium is further enriched by the addition of 7% Horse Blood. -
BM0160 Fastidious Anaerobe Broth is designed for the culture of fastidious anaerobes, particularly Bacteroides species, and is also suitable for use as an anaerobic blood culture medium. BM0160 is recommended by the UK Standards for Microbiology Investigations and is formulated and tested in compliance with the principals of ISO 11133:2014. Peptone and yeast extract supply the required carbon, nitrogen, and vitamins. Haemin, vitamin K, L-arginine, and L-cysteine HCl are additional growth factors required by some anaerobes. Sodium thioglycolate and L-cysteine HCl help lower the pH of the medium to maintain anaerobicity and starch and sodium hydrogen carbonate help neutralise toxins. The increased viscosity produced by addition of agar helps prevent oxygen diffusion in the final medium. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance.
-
This medium is for the growth of fastidious anaerobes, particularly Bacteroides spp. Fastidious anaerobe broth is also suitable for anaerobic blood culture. The peptone and yeast extract provides the required carbon, nitrogen and vitamins. Haemin, vitamin k and L-cysteine HCl are growth factors required by some anaerobes. Sodium thioglycollate and L-cysteine HCl reduce the Eh of the medium and the agar helps maintain the Eh. Resazurin is a redox indicator and sodium chloride maintains the osmotic balance. NB: For best results it is recommended that the medium be heated in a boiling water bath, with the cap loosened, and then allowed to cool, with the cap tightened, immediately before use. The cap must be replaced on the container immediately after inoculation.
-
A medium designed for optimum growth of anaerobes, particularly the fastidious organisms, it is generally used as an enrichment broth and can be used as a Blood Culture Medium. The growth factors Vitamin K, Haemin and L-Cysteine are included in the medium to assist those anaerobes that require them. L-Cysteine together with Sodium Thioglycollate reduces the ph of the medium and the Agar is present to reduce the absorption of Oxygen and convection currents. The redox indicator Resazurin is also present. The addition of glass beads will allow for the maceration of the sample and aid the homogenisation of the suspension. -
Ferric Ammonium Citrate (FAC) Supplement E&O Laboratories Ltd Ferric Ammonium Citrate (LS5004) is a supplement used in the isolation of Listeria spp. with Fraser Broth. Fraser Broth is a modification of the USDA-FSIS (United States Department of Agriculture-Food Safety Inspection Service) UVM secondary enrichment broth and is based on the formula described by Fraser and Sperber. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. All Fraser Broth enrichment cultures should be subcultured to plating medium. The medium is intended for the isolation of Listeria spp. from food and environmental samples when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. It is generally accepted that the USDA-FSIS two stage enrichment method employing UVM primary and secondary enrichment broths is the most suitable for the examination of meat products.
-
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. This formulation complies with the United States Pharmacopoeia. -
This broth is used to aid the rinsing of the membrane filter apparatus when performing sterility testing. Fluid D has been formulated with the addition of polysorbate 80 to enable the rinsing of products containing lecithin or oil. This formulation complies with the Harmonised USP/EP/JP.
-
This medium is intended for use in sterility testing of substances in accordance with the United States Pharmacopoeia and European Pharmacopoeia. It is a general purpose medium for the cultivation of both fastidious anaerobic and aerobic micro-organisms. Sodium Thioglycollate and L-cystine are included to reduce the medium to ensure anaerobiosis and to inactivate mercury and other heavy metallic compounds. A small amount of agar is added to further reduce the update of oxygen. However, in the event of oxidation, the medium will turn pink due to the presence of the resazurin. Providing only 30% of the medium has turned pink, the Eh may be restored by heating the medium (once only) in a boiling water bath. -
Four Vented 120mm Square Crystal Polystyrene Petri Dish Volume(s) : -
Four Vented 65mm Grip Lid Crystal Polystyrene Contact Dish Volume(s) : -
A modification of UVM Medium, Fraser Broth is a secondary selective enrichment broth for the isolation of Listeria spp primarily from food and environmental specimens. The medium is made selective by the inclusion of Nalidixic Acid and Acriflavine. Darkening of the broth following incubation, due to the presence of Aesculin and Ferric Ammonium Citrate, is indicative of the presence of Listeria spp. Lithium Chloride is also included to inhibit the growth of enterococci that would otherwise hydrolyse the Aesculin. This medium is generally used in conjunction with Fraser Broth Half-Strength (BM0647). NB: It should be noted that the lack of darkening of the broth should not be taken as a final negative result and all Fraser Broth enrichment cultures should be sub-cultured onto an appropriate selective agar medium irrespective of colour.
