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Originally introduced for the isolation and differentiation of Lactose & Non-Lactose Fermenting enteric organisms the medium in this case has been modified to improve the isolation of staphylococci and enterococci. The absence of Sodium Chloride provides a low electrolyte medium that prevents spreading of most Proteus spp. Although recommended for use in the examination of urine samples in clinical laboratories it has uses in Food, Water and Dairy applications. -
This is a medium for the cultivation of yeasts and moulds. The high carbohydrate content is said to ensure rapid growth while the low pH (5.4) inhibits most bacteria. Malt Extract Agar can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. -
A selective medium for the isolation of Staphylococcus aureus. The high level of Sodium Chloride inhibits most other organisms and as most Staphylococcus aureus ferment Mannitol the inclusion of Phenol Red indicator gives rise to yellow colonies. This formulation complies with the requirements of the Harmonised USP/EP/JP. NB: Some strains of coagulase negative staphylococci can give rise to false positive results on this medium It is necessary therefore to confirm the identity of Staphylococcus aureus by other confirmatory tests (e.g. Coagulase test, Latex test etc.) -
Middlebrooks 7H11 Selective Medium is an agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H10 Agar in that it has a higher concentration of Malachite Green. The medium is complex and includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. The medium is also made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
This medium is used for the presumptive identification of Pseudomonas aeruginosa from water and environmental samples. Pseudomonas aeruginosa is presumptively identified by the characteristic green pigmentation of the colonies with hydrolysis of casein (clear zones around each colony). -
Mueller Hinton Agar is recommended for use in the antibiotic disk diffusion method by both the European Committee on Antibiotic Sensitivity Testing (EUCAST) and the Clinical Laboratory Standards Institute (CLSI). The medium contains low levels of divalent metal cations, such as calcium and magnesium, to minimise any interference with certain antibiotic classes e.g. aminoglycosides. Starch is also present to absorb any toxic metabolites that may be formed during growth. The medium is low in thymine & thymidine content and is therefore suitable for use in testing sulphonamides and trimethoprim without the addition of blood. -
Approved by the Clinical Laboratory Standards Institute (CLSI) in USA this medium can be considered as an alternative to Iso-Sensitest Agar for antimicrobial sensitivity testing by disc diffusion methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim without the addition of Lysed Blood. -
Approved by the National Committee for Clinical Laboratory Standards (NCCLS) in USA this medium is approved for use in antimicrobial sensitivity testing by the disc diffusion method and is recommended particularly for use with the Bauer-Kirby Technique It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim and controlled to ensure correct zone sizes with Tetracyline and Aminoglycoside antibiotics. It can be considered as an alternative to Iso-Sensitest Agar. This particular formulation has an additional 2% Sodium Chloride added to the medium making it suitable for the detection of resistance to Methicillin in staphylococci and it is included in the recommendations of British Society for Antimicrobial Chemotherapy (BSAC) for this purpose. It is not however recommended for testing of organisms requiring a CO2 enriched environment due to the pH effect on the medium. If incubation in a CO2 enriched environment is essential control organisms should be included to confirm that results have not been altered. -
Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST). This medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Additional supplementation of the Mueller Hinton medium using 5% Horse Blood and 20mg/L of Nicotinamide adenine dinucleotide (NAD) makes it suitable for use with the more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae. -
Mueller-Hinton with 2% Glucose & Methylene Blue (25ml) This medium is intended for use as a means of differentiation of Candida spp. based on Mueller-Hinton Agar base. The medium is modified by the addition of Glucose and Methylene Blue indicator and is the recommended media for the susceptibility testing of Yeasts according to the CLSI M44-A2 document. -
A selective medium for the isolation of fungi, particularly dermatophytes from clinical specimens, Mycological Agar is suitable for use in all areas of Mycology. The medium inhibits most bacteria due to the addition of Chloramphenicol which is added to reduce the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction). -
Pages Amoeba Saline & Agar No.1 This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar. -
A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. -
This is an established medium, with a neutral pH, used for the enumeration of Yeasts and Moulds -
This is a selective differential medium for the isolation of Listeria monocytogenes from food, clinical and environmental specimens. -
Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar) This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation. -
This medium is recommended for the detection and enumeration of yeasts and fungi in a variety of sample types. The low pH (5.6) and addition of streptomycin will ensure that the growth of most bacterial species is inhibited and the low mineral content ensures good pigment production by fungi where appropriate. -
Chromogenic Coliform Agar (CCA) Chromogenic Coliform Agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique. The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide. Salmon-GAL is used for the detection of β-D-galactosidase enzymatic activity. X-glucuronide is used for the detection of β-D-Glucoronidase enzymatic activity. β-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies. Unlike other coliforms, Escherichia coli leaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colonies. Tryptophan is used to increase detection reliability by improving the indole reaction. The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms. Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance. The selectivity is attained by the addition of Tergitol® 7 as it inhibits the growth of Gram positive bacteria. -
Traditionally, membrane Lauryl Suphate Broth (mLSB) was used as the standard media for isolating coliforms (including E. coli) from drinking water. Primary membrane Lactose Glucuronide Agar (mLGA) is a chromogenic modification of mLSB formulation aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and <em,>E. coli . The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. X-glucuronide is incorporated to allow for the presumptive isolation of E. coli, sodium pyruvate aids recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads. This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Environment Agency’s - The Microbiology of Drinking Water 2009 (Part 4). -
Tryptone Bile X (TBX) - Glucuronide Agar Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available. -
Side 1: Primary UTI Chromogenic Agar This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of enterococci (turquoise colonies), Proteus spp (clear colonies with a brown halo), Enterobacter spp (metallic blue colonies), staphylococci (white colonies), and E. coli (purple colonies). Side 2: Columbia Agar w 7% Defibrinated Horse Blood & CNA This is a selective medium for the isolation of Staphylococcus/ spp and Streptococcus spp. It is based on Columbia Agar enriched with defibrinated horse blood which promotes good colony appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. The medium is made selective by the inclusion of colistin and nalidixic acid to suppress growth of the majority of Gram-negative bacteria. -
This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of Enterococcus spp. (turquoise colonies), Proteus spp. (clear colonies with a brown halo), Enterobacter spp. (metallic blue colonies), Staphylococcus spp. (white colonies), and E. coli (purple colonies). -
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections. Based on the traditional CLED Medium, to prevent the swarming of Proteus spp, two chromogens are present in the medium. One allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and Tryptophan are also included as indicators of Tryptophan deaminase activity producing brown colonies of Proteus spp. This media is an opaque version to aid differentiation and presumptive identification of the bacteria isolated. -
This is a medium for the isolation and identification of Group B streptococci. The principal of the medium is based on the ability of group B streptococci to produce unique orange/red pigmented colonies when incubated anaerobically, particularly on media containing starch products. This medium is non-selective so other organisms will grow on this medium but they do not produce the characteristic pigment. -
Pseudomonas Agar Base with 1% Glycerol, Cephalothin, Fucidin & Cetrimide (CFC) This is a selective medium for the isolation of Pseudomonas spp primarily in food, water and environmental samples. The medium uses Magnesium and Potassium salts to enhance pigment production and is made selective by the addition of CFC supplement. The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism. -
Pseudomonas Agar Base with 1% Glycerol & CN Supplement A selective medium for the isolation of Pseudomonas aeruginosa the medium is made selective by the inclusion of Cetrimide and Naladixic Acid (CN) supplement to significantly reduce the enteric organisms particularly Proteus and Klebsiella spp. Magnesium and Potassium salts are included to enhance the production of the pigments pyocyanin and fluorescein. -
A selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples the medium is made selective by the addition of CFC supplement (cetrimide, at a concentration of 10mg/L which is said to allow the growth of all pseudomonads, cephalothin and fucidin). The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism. -
R2A medium was developed to determine the bacterial count including heterotrophic bacteria in potable waters during treatment and distribution. This medium has a low nutritional content and therefore requires extended incubation times. It is recommended by the Environmental Agency, Methods for the Examination of Waters and Associated Materials, and Standard Methods for the Enumeration of Water and Wastewater. -
RPMI Medium for E-Test RPMI Medium is recommended for use in anti-fungal susceptibility testing of yeasts from clinical isolates using the E-Test method. The medium is based on a simple Glucose Agar with added RPMI-1640 Medium (without Sodium Bicarbonate & Phenol Red), which supplies the necessary vitamins and amino-acids, and MOPS (3-(Morpholino)propanesulfonic Acid) Buffer to maintain the medium pH during incubation. -
Sabouraud Dextrose Agar with Chloramphenicol (0.5g/L) is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1) The tryptone and meat peptone provides the required nitrogen, carbon and vitamins. The high concentration of dextrose is included as an energy source. Due to the higher pH of the medium, an increased concentration of chloramphenicol is included to improve the selectivity of the media and inhibit a range of Gram-positive and Gram negative bacteria. 1. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061. -
This is one of several media available for the selective isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. NB: This is a basic medium only and contains no additional supplements. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the Harmonized USP/EP/JP. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia & European Pharmacopoeia. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy -
Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) A selective medium for the isolation of yeasts and fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria, however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. -
Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) & Cyclohexamide (Actidione) (300mg/L) A selective medium for the isolation of fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
This is a modification of Sabouraud Dextrose Agar. The low pH (5.6) of the medium is inhibitory to most bacteria and it has been made specifically selective by the addition of colistin and gentamicin. This further reduces the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Gram negative organisms such as Pseudomonas aeruginosa. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin & Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds & Tween neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
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Sabouraud Dextrose Agar with Lecithin, Tween 80, Histidine & Sodium Thiosulphate (VHP) (USP) - Irradiated This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin, Polysorbate 80 (Tween 80), Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol and Sodium Thiosulphate inactivate mercurials). This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
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Originally intended as a medium for the enumeration of enterococci in water using Membrane Filtration, this medium has become more popular in many other areas such as food bacteriology. The medium contains Tetrazolium Chloride, which is reduced by enterococci to the insoluble red dye Formazan resulting in dark red colonies of enterococci on the agar. It should be noted that this reaction is not exclusive to enterococci and colonies should be confirmed by additional testing e.g. Aesculin hydrolysis. -
This is a differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey media in that lactose has been replaced by sorbitol. As Escherichia coli 0157:H7 does not ferment sorbitol, it produces pale translucent colonies whereas most other strains of Escherichia coli do ferment sorbitol and produce pink colonies. -
Sorbitol MacConkey with Cefixime & Tellurite (CT-Smac) This is a selective differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey medium in that Lactose has been replaced by Sorbitol. As Escherichia coli 0157:H7 does not ferment Sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are Sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial Cefixime and Potassium Tellurite. -
Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C. NB - It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium. -
This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction. -
Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains. -
This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks. -
This is a general-purpose medium which supports the growth of a wide range of organisms. It is suitable for Phage Typing, Colicine Typing and for testing the X and V requirements of Haemophilus spp as well as many other areas of bacteriological investigation and conforms to the requirements of the United States Pharmacopeia for sterility testing of pharmaceutical products. -
This is a general-purpose complex medium for the cultivation and isolation of aerobic and anaerobic microorganisms. The base medium, Tryptone Soya Agar (Soybean-Casein Digest agar), conforms to the Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP).The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy -
This is a general-purpose complex medium for cultivation and isolation of fastidious bacteria, yeasts and moulds. The formulation is based on the United States Pharmacopoeia (USP Medium II) and European Pharmacopoeia (EP Medium B). The medium can be incubated under aerobic or anaerobic conditions for sterility testing, air sampling and other areas of bacteriological investigation. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. This particular product is triple wrapped and terminally sterilised by Gamma irradiation. Dose Range: 8.0 kGy - 15.0kGy
