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This is a selective medium primarily for the isolation of Enterobacteriacae from waters & sewage. This media differs from the original MacConkey formulation in that as well as Bile Salts, Crystal Violet has been included as an additional selective agent. This has the effect of inhibiting gram-positive micrococci. -
MacConkey agar No. 3 is a selective medium primarily for the isolation of Enterobacteriacae from waters and sewage. This media differs from the original MacConkey formulation in that as well as bile salts, crystal violet has been included as an additional selective agent. This has the effect of inhibiting Gram-positive organisms. The peptone acts as a nitrogen, carbon and vitamin source. Sodium chloride maintains osmotic balance. Lactose is a carbohydrate and during its fermentation causes a confined pH drop around the bacterial colony. This causes a colour change in the pH indicator, neutral red and bile precipitation. -
KM0124 MacConkey Agar with Salt is a selective medium for the isolation and differentiation of bile tolerant Gram-negative (enteric) and Gram-positive (staphylococci and enterococci) organisms in all areas of bacteriology. MacConkey Agar with Salt is based on the formulation by MacConkey in 1900. Staphylococcus and Enterococcus spp. are able to grow due to the omission of crystal violet from this formulation. This medium is recommended by the World Health Organisation and the Department of Health for the bacteriological examination of water, and KM0124 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations.
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Originally introduced for the isolation and differentiation of Lactose & Non-Lactose Fermenting enteric organisms the medium in this case has been modified to improve the isolation of staphylococci and enterococci. The absence of Sodium Chloride provides a low electrolyte medium that prevents spreading of most Proteus spp. Although recommended for use in the examination of urine samples in clinical laboratories it has uses in Food, Water and Dairy applications. -
MacConkey Agar without Salt and Crystal Violet, based on the formulation by Rappaport and Henig, is a differential medium for the isolation and differentiation of lactose and non-lactose fermenting enteric bacteria while also restricting the swarming of Proteus species. KM0011 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations, The Microbiology of Drinking Water and tested in accordance with ISO 11133:2014. Related Supplements : LS0189 Cefotaxime Supplement (1mg/L)
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A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP. -
Malt extract is sourced from hydrolyzed vegetal protein. Due to the high concentration of carbohydrate malt extract is particularly suited for the cultivation of yeasts and moulds especially in contaminated dairy products. In microbiological culture media it provides a source of nutrients, protein, and carbon.
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Malt extract agar is used for the detection, isolation and enumeration yeasts and moulds. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds. Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth. Malt extract agar can also be used for the cultivation of fungi, although with the prolonged incubation necessary, cultures may become overgrown by bacteria. The selectivity of the medium can be increased by lowering the pH to 3.5-4.0 or by the addition of selective agents such as chloramphenicol. Related Supplements : LS0050 Chloramphenicol Selective Supplement
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This is a medium for the cultivation of yeasts and moulds. The high carbohydrate content is said to ensure rapid growth while the low pH (5.4) inhibits most bacteria. Malt Extract Agar can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. -
Malt extract broth is used for the cultivation of yeasts and moulds and is commonly used as part of sterility testing protocols. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds. Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth.
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KM0031 Mannitol Salt Agar is a selective medium for the isolation of Staphylococcus aureus from clinical samples, food, cosmetics and water samples. Chapman showed that adding a higher level of sodium chloride to Mannitol Salt Agar allowed for the recovery of pathogenic staphylococci and inhibits most organisms. Coagulase positive staphylococci (e.g., S. aureus) produce yellow colonies and a surrounding yellow medium while coagulase negative staphylococci produce red colonies and no colour change of the phenol red indicator. The medium conforms to the requirements of the Harmonised EP/USP/JP, is recommended as a primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with ISO 11133:2014 and Clinical and Laboratory Standards Institute. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing.
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A selective medium for the isolation of Staphylococcus aureus. The high level of Sodium Chloride inhibits most other organisms and as most Staphylococcus aureus ferment Mannitol the inclusion of Phenol Red indicator gives rise to yellow colonies. This formulation complies with the requirements of the Harmonised USP/EP/JP. NB: Some strains of coagulase negative staphylococci can give rise to false positive results on this medium It is necessary therefore to confirm the identity of Staphylococcus aureus by other confirmatory tests (e.g. Coagulase test, Latex test etc.) -
BM0370 Selenite Mannitol Broth is a selective enrichment medium used for the isolation of Salmonella species from clinical, food and environmental specimens. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media. Selenite Mannitol Broth is recommended for the enrichment of Salmonella species from faecal specimens in UK SMI S7 for the investigation of gastroenteritis. -
Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent. -
Formulated to ISO 6887-1, Maximum Recovery Diluent (MRD) is a diluent designed to maintain organisms by protecting the cells from unnecessary physiological shock that may occur using other aqueous solutions. MRD is used extensively in food and environmental testing. The low level of peptone provides a protective effect but does not allow for multiplication during the short residence time in the diluent, 45 minutes. The sodium chloride prevents osmotic shock as the sample is initially diluted.
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Meat peptone is sourced from hydrolyzed proteins of animal tissue. This peptone consists of soluble amino acids and peptides that provide readily available nitrogen and other essential growth factors. It is primarily used for the cultivation of fastidious and non-fastidious microorganisms in microbiological culture media.
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2 port media bag for use with large capacity liquid products Volume(s) : 5L -
Medical Flat Bottle with a 200ml volume capacity and a 28mm polypropylene cap Volume(s) : 200ml -
For the enumeration of Escherichia coli and coliform organisms in water using a Membrane Filtration Technique. Previously known as Membrane Enriched Teepol Broth, Lauryl Sulphate has replaced Teepol 610, which is no longer available. Phenol Red is included in the medium making it possible for coliforms to be more readily detected following incubation.
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Middlebrooks 7H11 Selective Medium is an agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H10 Agar in that it has a higher concentration of Malachite Green. The medium is complex and includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. The medium is also made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
This medium is used for the presumptive identification of Pseudomonas aeruginosa from water and environmental samples. Pseudomonas aeruginosa is presumptively identified by the characteristic green pigmentation of the colonies with hydrolysis of casein (clear zones around each colony). -
A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. This medium allows for the detection of gas when a Durhum tube is incorporated in the medium. -
A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. Which allows the detection of acid & gas when a Durhum tube is incorporated in the medium. -
Modified Campylobacter (Bolton) Selective Supplement E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens. -
This is best described as a multi-purpose medium for differentiation of enterobacteriacae that combines three individual tests into a single medium. For use the medium is inoculated by making a single stab into the medium with a straight wire (or equivalent) using a pure culture (or discrete single colony) of the test organism. Following incubation it is recommended that the medium should first of all be examined to determine whether or not the organism is motile. The presence of motility is apparent by the organism tracking out from the line of inoculation and often turning the medium turbid. Non-motile organisms generally grow within the stab line leaving the surrounding medium clear. Urease positive organisms (e.g. Proteus spp) turn the medium bright red due to the hydrolysis of the Urea in the presence of the Phenol Red Indicator often making it difficult to determine the other parameters.Indole is tested for by layering a small amount of Indole Reagent (Erlich’s or Kovac’s appear to work equally well) onto the surface of the medium and allowed a few minutes to react. A positive result is indicated by the formation of a red line at the interface of the reagent and the medium. -
de Man, Rogosa & Sharpe (MRS) Agar M.R.S. Agar is intended for the cultivation and enumeration of Lactobacillus spp from a variety of sources and can be used as an alternative to Orange Serum Agar for that purpose. Magnesium Sulphate, Manganese Sulphate, Sodium Acetate and Tween are included as growth supplements. The medium can be made more specific for lactobacilli generally by lowering the pH to between 5.0 and 5.5. This has the effect of inhibiting most streptococci that may otherwise grow on the medium and can be readily confused with lactobacilli. -
KM0183 Mueller Hinton Agar is used as a base to make various media for antimicrobial susceptibility testing by the disk diffusion method as described by Bauer-Kirby. First described by Mueller and Hinton, Mueller Hinton Agar has been approved as the definitive medium for antimicrobial susceptibility testing by European Committee on Antimicrobial Susceptibility Testing (EUCAST), Clinical and Laboratory Standards Institute (CLSI) and is compliant with ISO16782:2016. KM0183, when prepared as unsupplemented Mueller Hinton Agar, is suggested by EUCAST and CLSI for non-fastidious organisms such as Enterobacterales, Pseudomonas spp., Stenotrophomonas maltophilia, Acinetobacter spp., Staphylococcus spp., Enterococcus spp., Aeromonas spp., Achromobacter xylosoxidans, Vibrio spp., Bacillus spp. and Burkholderia pseudomallei. KM0183, when prepared supplemented with 5% horse blood and nicotinamide adenine dinucleotide (NAD), is suggested by EUCAST and CLSI for fastidious organisms such as Streptococcus pneumoniae, Streptococcus groups A, B, C and G, Viridans group streptococci, Haemophilus influenzae, Moraxella catarrhalis, Listeria monocytogenes, Pasteurella multocida, Campylobacter jejuni and coli, Corynebacterium spp., Aerococcus sanguinicola and urinae and Kingella kingae. Additionally, according to the UK Standards for Microbiology Investigations, when supplemented appropriately, Mueller Hinton Agar can be used in the process of identifying Haemophilus species and the HACEK group of organisms and Helicobacter species from clinical specimens. Related Supplements : LS0005 NAD Supplement (20mgs/L), Defibrinated Horse Blood
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Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST). This medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Additional supplementation of the Mueller Hinton medium using 5% Horse Blood and 20mg/L of Nicotinamide adenine dinucleotide (NAD) makes it suitable for use with the more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae. -
Approved by the Clinical Laboratory Standards Institute (CLSI) in USA this medium can be considered as an alternative to Iso-Sensitest Agar for antimicrobial sensitivity testing by disc diffusion methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim without the addition of Lysed Blood. -
Approved by the National Committee for Clinical Laboratory Standards (NCCLS) in USA this medium is approved for use in antimicrobial sensitivity testing by the disc diffusion method and is recommended particularly for use with the Bauer-Kirby Technique It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim and controlled to ensure correct zone sizes with Tetracyline and Aminoglycoside antibiotics. It can be considered as an alternative to Iso-Sensitest Agar. This particular formulation has an additional 2% Sodium Chloride added to the medium making it suitable for the detection of resistance to Methicillin in staphylococci and it is included in the recommendations of British Society for Antimicrobial Chemotherapy (BSAC) for this purpose. It is not however recommended for testing of organisms requiring a CO2 enriched environment due to the pH effect on the medium. If incubation in a CO2 enriched environment is essential control organisms should be included to confirm that results have not been altered. -
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar. -
Mueller-Hinton with 2% Glucose & Methylene Blue (25ml) This medium is intended for use as a means of differentiation of Candida spp. based on Mueller-Hinton Agar base. The medium is modified by the addition of Glucose and Methylene Blue indicator and is the recommended media for the susceptibility testing of Yeasts according to the CLSI M44-A2 document. -
This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella spp. reduce tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional tetrathionate broth the addition of novobiocin (40 mg/l) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 20 ml/l of 2% iodine/iodide solution (BM0946). Once the iodine/iodide solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. Related Supplements : LS0024 Novobiocin Supplement (20mgs/L)
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This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
Mycological agar is a selective medium for the isolation of pathogenic fungi, particularly dermatophytes, from clinical specimens. Enzymatic digest of soybean meal provides the required carbon, nitrogen and vitamins. Glucose is an energy source for the metabolism of fungi. The addition of chloramphenicol further reduces the risk of bacterial contamination when processing material that may be heavily contaminated with coliforms. Cycloheximide should also be added (0.4g/L) to suppress the growth of commensal yeasts and saprophytic fungi.
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A selective medium for the isolation of fungi, particularly dermatophytes from clinical specimens, Mycological Agar is suitable for use in all areas of Mycology. The medium inhibits most bacteria due to the addition of Chloramphenicol which is added to reduce the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction). -
Neomycin Selective Supplement E&O Laboratories Ltd Neomycin Selective Supplement (LS0017) is an antibiotic supplement used to enhance the isolation of anaerobes from clinical specimens.
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BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis. BM8200 Non-acidified Glycerol Lowenstein Jensen Medium is designed for use with sodium hydroxide (NaOH) treated specimens that have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto the LJ medium after concentration by centrifugation. This medium will isolate most common mycobacteria including M. tuberculosis and can be used for the presumptive identification of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy). BM8200 is recommended by the UK Standards for Microbiology Investigations. NB: This media will not isolate wild strains of M. bovis, which requires the addition of pyruvate as a growth supplement. This is normally overcome by the inclusion of a pyruvate slope such as E&O BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium. -
BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium bovis. BM8300 Non-acidified Lowenstein Jensen Pyruvate Medium is designed for use with sodium hydroxide (NaOH) treated samples which have been neutralised using known buffering solutions to achieve a pH of 6.8 to 7.0 and which are inoculated directly onto LJ media after concentration by centrifugation. This medium differs from E&O BM8200 Non-acidified Glycerol Lowenstein Jensen Medium in that sodium pyruvate has replaced the glycerol, which has been demonstrated to be inhibitory to some species, particularly M. bovis. BM8300 is recommended by the UK Standards for Microbiology Investigations and tested in accordance with the principals of ISO 11133:2014. The medium contains an egg emulsion which provides the required protein and fatty acids. Sodium pyruvate is included as an alternate to glycerol to allow favourable growth of M. bovis. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green and penicillin G are incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen in the specimen after decontamination. Lowenstein-Jensen slopes should be inoculated with pre-treated specimens and incubated at 36 ± 1°C for 8 weeks, checking weekly for growth. Further incubation up to 12 weeks may be required for certain Mycobacterium species. -
For in vitro diagnostic use only. PP2160 Non-Nutrient Agar is a non-nutrient medium recommended for the detection of free-living pathogenic Acanthamoeba and Naegleria spp. cysts, trophozoites, and flagellates in tissue, soil, or water samples. Non-Nutrient Agar is based on Page's Saline solidified with 1.5% agar. Page's Saline (Amoeba Saline) was developed by Page in the 1960’s(1) to provide a suitable environment for the isolation and cultivation of amoebae, particularly free-living amoebae. These amoebae are commonly found in diverse habitats such as soil, water bodies, and human tissues. Non-Nutrient Agar has a balanced salt content that mimics the osmotic environment found in the body fluids of animals, making it suitable for the cultivation and study of a wide range of microorganisms. Agar is the solidifying agent. -
Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.
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A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. When used to prepare agar slopes or agar butts, the medium can be used to maintain control organisms. The peptone provides the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.
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For in vitro diagnostic use. BM0540 Nutrient Agar is a basic general-purpose medium suitable for use in the cultivation and subculture of the less fastidious organisms. Nutrient agar was first described by the American Public Health Association (APHA) in 1917. It is used to check the purity of subcultures from isolation plates prior to biochemical or serological tests. It is particularly useful for the cultivation of the less fastidious organisms, particularly those that do not require the addition of blood or other enrichment. It is one of the most important and commonly used non-selective media for the routine cultivation of microorganisms. Nutrient Agar slopes can be used to maintain organisms as it allows prolonged survival of cultures at ambient temperature without the risk of overgrowth that might occur with more nutritious mediums. -
A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. -
BM0550 Nutrient Broth is a basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms. Nutrient broth is based on the recipe for Nutrient Agar, which was first described by the American Public Health Association (APHA) in 1917 as a medium to cultivate a wide variety of organisms. Although, a basic general-purpose medium, this medium can be enriched with other ingredients such as blood, serum, sodium chloride, sugars, etc., for special purposes. -
A general-purpose medium for the cultivation of organisms that are less fastidious in their nutritional requirements. The beef extract, peptone and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains osmotic balance.
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Nutrient Broth No.2 is used for the cultivation of fastidious pathogens and other microorganisms. This general use medium, rich in nutrients, allows the growth of bacteria when there is a low level of cells. The medium is particularly suitable as a secondary growth medium for staphylococci to be tested for coagulase production and also be used for sterility testing of aerobic organisms. Beef extract and peptone provide the required carbon, nitrogen and vitamins. Sodium chloride maintains osmotic balance.
