-
Letheen broth when prepared with polysorbate 80 is used for the testing of quaternary ammonium compounds for antimicrobial activity. Letheen broth is recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC). (1) The enzymatic digest of animal tissue and beef extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains the osmotic balance of the medium. Lecithin inactivates quaternary ammonium compounds. Polysorbate 80 must be added to the medium prior to sterilisation. Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol. REFERENCE (1) Association of Official Analytical Chemists. 1995. Official methods of analysis, 16th ed. Association of Official Analytical Chemists, Washington, D.C -
Letheen broth modified is intended for use in the isolation of microorganisms from cosmetics. The enzymatic digest of animal tissue, enzymatic digest of casein, yeast extract and beef extract act as carbon, nitrogen and vitamin sources in this medium. Sodium chloride maintains the osmotic balance of the medium. The sodium bisulfite and lecithin inactivates quaternary ammonium compounds. Polysorbate 80 must be added to the medium prior to sterilisation. Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.
-
Letheen Broth with Neutraliser and 1% Tween 80 is primarily intended for use in assessing the bactericidal activity of quaternary ammonium compounds and determining the phenol coefficient of cationic surfactants. It can also be used in environmental testing, particularly in areas subjected to surface disinfection. Lecithin and polysorbate 80 (Tween 80) inactivate surface disinfectants (lecithin neutralises quaternary ammonium compounds and Tween 80 neutralises phenols, formalin, hexachlorophene and in combination with the lecithin ethanol). -
LIM Broth with 10% Serum A nutritious, selective broth medium utilising the base formulation developed by Todd and Hewitt for the enrichment of Group B Streptococci. The LIM broth is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of other bacteria. Serum is also included to enhance the nutritional qualities of the base medium. -
One of several media available as a selective identification medium for the isolation and identification of Listeria monocytogenes in food & clinical laboratories. Using Columbia Agar as the base Lithium Chloride is included to inhibit enterococci and Acriflavine to inhibit some other gram positive and gram negative organisms that may be present in such specimens. It is made further selective by the addition of the antimicrobials Cefoxitin, Colistin & Fosfomycin with Amphotericin included to inhibit any yeasts present. Aesculin is present as an indicator since Listeria monocytogenes will hydrolyse it and the associated reaction with the Ferric Ammonium Citrate gives rise to a black precipitate around the colonies. -
Listeria isolation medium (Oxford) is based on the formulation described by Curtis et al. and is used for the isolation and identification of Listeria spp. in food and clinical laboratories. Columbia agar base provides the required carbon, nitrogen and vitamins in the medium. Lithium chloride is included to inhibit enterococci. Aesculin is present as an indicator; Listeria spp. will hydrolyse aesculin and the associated reaction with the ferric ammonium citrate gives rise to a black precipitate around the colonies. Selectivity is enhanced by addition of Listeria Oxford selective supplement (LS0030). This contains acriflavine, cefoxitin, colistin, fosfomycin and amphotericin to inhibit any yeasts present and some other Gram-positive and Gram-negative organisms that may be present in specimens. Related Supplements : LS0030 Listeria Oxford Selective Supplement
-
Listeria Oxford Selective Supplement E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples. -
Long and Hammer agar is for the detection of aerobic microorganisms in fish and fish products. This media is recommended for the determination of spoilage organisms in fresh and lightly preserved seafoods. (1) The proteose peptone acts as a nitrogen, carbon and vitamin source. Gelatine is a protein source and solidifying agent. Sodium chloride maintains the osmotic balance and di-potassium hydrogen phosphate is a buffer. 1) Nordic Committee on Food Analysis. (2006) Aerobic count and specific spoilage organisms in fish and fish products. NMKL Method No 184
-
This is an egg-based medium for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis, from clinical samples. Based on the original formulation of Lowenstein that was subsequently modified by Jensen, the medium contains glycerol and egg which provide the required protein and fatty acids. The coagulation of the egg albumin during preparation also provides a solid surface for inoculation purposes. Malachite green is incorporated into the medium to inhibit contaminating organisms other than the mycobacteria that may still be present in the specimen after decontamination. Lowenstein Jensen Medium slopes should be inoculated with pre-treated specimens and incubated at 35-37°C for 8 weeks in 5-10% CO2. Container caps should be left loose for the first week of incubation to allow for circulation of the carbon dioxide as this will help to stimulate growth. Caps should then be tightened to prevent any dehydration of the medium -
Lowenstein Jensen Pyruvate Medium is an egg-based medium for the cultivation of Mycobacterium spp., particularly Mycobacterium bovis. Lowenstein Jensen Pyruvate Medium is based on the original formulation of Löwenstein that was later modified by Jensen. It differs from Lowenstein Jensen Medium (BM0200) in that sodium pyruvate has replaced the glycerol, which has been demonstrated to be inhibitory to some species, particularly M. bovis. Lowenstein Jensen Pyruvate Medium can be used for the diagnosis of mycobacterial infections, testing antibiotic susceptibility of isolates, and differentiating different species of Mycobacterium (by colony morphology, growth rate, biochemical characteristics, and microscopy). It is recommended by Public Health England as a standard media for the investigation of specimens for Mycobacterium species. The coagulation of the egg albumin during preparation provides a solid surface for inoculation purposes. L-Asparagine and soluble starch are sources of nitrogen and vitamins. Potassium di-hydrogen phosphate and magnesium sulphate enhance organism growth and act as buffers. Sodium pyruvate is added to serve as a carbon source in place of glycerol. The egg mixture also provides essential fatty acids and protein to support the growth of mycobacteria. Malachite green is incorporated into the medium to exert an inhibitory effect on organisms other than mycobacteria.
-
Lowenstein-Jensen medium is based on the original formulation of Lowenstein that was subsequently modified by Jensen. It is used with fresh egg and glycerol for the isolation and presumptive identification of Mycobacterium spp., particularly Mycobacterium tuberculosis, from clinical samples. L-Asparagine and Potato starch are sources of nitrogen and vitamins in the medium. Potassium hydrogen phosphate and magnesium sulphate enhance organism growth and act as buffers. Sodium citrate and malachite green are selective agents that have inhibitory effects on organisms other than the mycobacteria. Malachite green is also incorporated into the medium to provide a colour contrast between the colonies and the medium. The required addition of egg emulsion provides the fatty acids and protein required for the metabolism of mycobacteria. Glycerol is also added which is said to enhance the growth of Mycobacterium tuberculosis however other strains of mycobacteria, particularly Mycobacterium bovis, may be inhibited by its presence. Subsequently, sodium pyruvate (12.5 g/600ml) may be used as an alternative to glycerol to encourage the growth of Mycobacterium bovis.
-
Lysine iron agar is a differential medium for the detection of Salmonella spp. and other enteric pathogens on the basis of lysine decarboxylase and hydrogen sulphide production. Lysine iron agar was originally developed by Edwards and Fife(1) for Salmonella arizonae detection. The peptone and yeast extract provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. L-lysine is used to detect lysine decarboxylase and lysine deaminase enzymes. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator. Sodium chloride maintains the osmotic balance. Bromocresol purple is a pH indicator. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
-
This is a selective medium for the isolation and differentiation of bile tolerant gram-negative (enteric) and gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology. It has the disadvantage that many strains of Proteus spp will spread on it and for this reason MacConkey Agar without Salt may be preferred. -
This is a selective medium primarily for the isolation and differentiation of coliforms and non-lactose fermenting Gram –ve bacterial species from clinical, dairy, industrial and water samples. This media differs from the original MacConkey formulation with the addition of an extra 0.5% Sodium chloride, an altered Bile salt mix and the neutral red concentration was also modified. This enhanced the effect of inhibiting Gram +ve and other non-enteric bacterial species. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
This is a selective medium primarily for the isolation of Enterobacteriacae from waters & sewage. This media differs from the original MacConkey formulation in that as well as Bile Salts, Crystal Violet has been included as an additional selective agent. This has the effect of inhibiting gram-positive micrococci. -
MacConkey agar No. 3 is a selective medium primarily for the isolation of Enterobacteriacae from waters and sewage. This media differs from the original MacConkey formulation in that as well as bile salts, crystal violet has been included as an additional selective agent. This has the effect of inhibiting Gram-positive organisms. The peptone acts as a nitrogen, carbon and vitamin source. Sodium chloride maintains osmotic balance. Lactose is a carbohydrate and during its fermentation causes a confined pH drop around the bacterial colony. This causes a colour change in the pH indicator, neutral red and bile precipitation.
-
KM0124 MacConkey Agar with Salt is a selective medium for the isolation and differentiation of bile tolerant Gram-negative (enteric) and Gram-positive (staphylococci and enterococci) organisms in all areas of bacteriology. MacConkey Agar with Salt is based on the formulation by MacConkey in 1900. Staphylococcus and Enterococcus spp. are able to grow due to the omission of crystal violet from this formulation. This medium is recommended by the World Health Organisation and the Department of Health for the bacteriological examination of water, and KM0124 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations.
-
Originally introduced for the isolation and differentiation of Lactose & Non-Lactose Fermenting enteric organisms the medium in this case has been modified to improve the isolation of staphylococci and enterococci. The absence of Sodium Chloride provides a low electrolyte medium that prevents spreading of most Proteus spp. Although recommended for use in the examination of urine samples in clinical laboratories it has uses in Food, Water and Dairy applications. -
MacConkey Agar without Salt and Crystal Violet, based on the formulation by Rappaport and Henig, is a differential medium for the isolation and differentiation of lactose and non-lactose fermenting enteric bacteria while also restricting the swarming of Proteus species. KM0011 is recommended as a differential primary isolation media by the UK Standards for Microbiology Investigations, The Microbiology of Drinking Water and tested in accordance with ISO 11133:2014. Related Supplements : LS0189 Cefotaxime Supplement (1mg/L)
-
This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth.
-
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of bromocresol purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of ox bile helps to suppress the growth of Gram-positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP. Nitrogen is supplied by the gelatin peptone whilst lactose serves as the fermentable carbohydrate source. Oxbile is the selective agent helping to suppress Gram-positive organisms and bromocresol purple detects the pH change as a result of the fermentation of lactose.
-
A selective medium for the enrichment of Enterobacteriaceae and the detection of Escherichia coli in pharmaceutical products. The inclusion of Bromocresol Purple indicator makes the colour change caused by acid production from the fermentation of lactose easy to read with gas formation. The presence of Ox bile helps to suppress the growth of Gram positive and non-enteric bacterial species. This formulation complies with the Harmonized USP/EP/JP.
-
Malt extract is sourced from hydrolyzed vegetal protein. Due to the high concentration of carbohydrate malt extract is particularly suited for the cultivation of yeasts and moulds especially in contaminated dairy products. In microbiological culture media it provides a source of nutrients, protein, and carbon.
-
Malt extract agar is used for the detection, isolation and enumeration yeasts and moulds. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds. Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth. Malt extract agar can also be used for the cultivation of fungi, although with the prolonged incubation necessary, cultures may become overgrown by bacteria. The selectivity of the medium can be increased by lowering the pH to 3.5-4.0 or by the addition of selective agents such as chloramphenicol. Related Supplements : LS0050 Chloramphenicol Selective Supplement
-
Malt Extract Agar is a medium for the isolation of many yeasts and moulds. The low pH inhibits most bacteria and further selectivity can be achieved by lowering the pH even more by adding lactic acid to the molten cooled, medium. This formulation is customer specific with a request for an additional 5g/L of agar from the customer. It should be noted that excess heating of the medium could result in hydrolysis of the agar resulting in softening of the agar.
-
This is a medium for the cultivation of yeasts and moulds. The high carbohydrate content is said to ensure rapid growth while the low pH (5.4) inhibits most bacteria. Malt Extract Agar can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. -
Malt Extract Agar (pH 5.6) is recommended for the detection and isolation and enumeration of yeasts and moulds (1). The medium is also used in the testing of foods and various materials and for the cultivation of the strains for microbiological vitamin assays. Malt Extract Agar (pH 5.6) has been used to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and foods.
This medium meets the requirements of The British Standards Institution (2).
References- Galloway, L. and Burgess, R. (1952) Applied Mycology and Bacteriology, Leonard Hill, London. Thom and Church, 1926. The Aspergilli.
- The British Standards Institution (2015) BS EN 16615:2015 Chemical disinfectants and antiseptics. Quantitative test method for the evaluation of bactericidal and yeasticidal activity on non-porous surfaces with mechanical action employing wipes in the medical area (4- field test). Test method and requirements (phase 2, step 2). Published by BSI Standards Limited.
-
Malt extract broth is used for the cultivation of yeasts and moulds and is commonly used as part of sterility testing protocols. The high carbohydrate content of the medium ensures rapid growth of yeasts and moulds. Malt extract and mycological peptone provide the carbon, protein and nutrient sources required by the organisms. The acidic nature of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth.
-
KM0031 Mannitol Salt Agar is a selective medium for the isolation of Staphylococcus aureus from clinical samples, food, cosmetics and water samples. Chapman showed that adding a higher level of sodium chloride to Mannitol Salt Agar allowed for the recovery of pathogenic staphylococci and inhibits most organisms. Coagulase positive staphylococci (e.g., S. aureus) produce yellow colonies and a surrounding yellow medium while coagulase negative staphylococci produce red colonies and no colour change of the phenol red indicator. The medium conforms to the requirements of the Harmonised EP/USP/JP, is recommended as a primary isolation media by the UK Standards for Microbiology Investigations and tested in accordance with ISO 11133:2014 and Clinical and Laboratory Standards Institute. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing.
-
A selective medium for the isolation of Staphylococcus aureus. The high level of Sodium Chloride inhibits most other organisms and as most Staphylococcus aureus ferment Mannitol the inclusion of Phenol Red indicator gives rise to yellow colonies. This formulation complies with the requirements of the Harmonised USP/EP/JP. NB: Some strains of coagulase negative staphylococci can give rise to false positive results on this medium It is necessary therefore to confirm the identity of Staphylococcus aureus by other confirmatory tests (e.g. Coagulase test, Latex test etc.) -
BM0370 Selenite Mannitol Broth is a selective enrichment medium used for the isolation of Salmonella species from clinical, food and environmental specimens. Following overnight incubation subculture(s) are usually made on to one or more of the many selective enteric solid media. Selenite Mannitol Broth is recommended for the enrichment of Salmonella species from faecal specimens in UK SMI S7 for the investigation of gastroenteritis. -
Marine agar is a medium used to support the growth of numerous marine bacteria. Originally formulation by ZoBell (1), its high saline levels are to simulate a close approximation of seawater which allows the marine bacteria to grow (2). The sodium chloride and marine minerals provide the high salinity needed for marine bacteria to thrive and aims to simulate sea water. The peptones provide the necessary nitrogen, amino acids and vitamins for growth. Agar is the solidifying agent. References (1) ZoBell, C.E. 1941. Studies on Marine Bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4, 42-75. (2) Lyman, J. and Fleming, R. H. 1940. Composition of Seawater. J. Mar. Res. 3, 134-146
-
Formulated to ISO 6887-1, Maximum Recovery Diluent (MRD) is a diluent designed to maintain organisms by protecting the cells from unnecessary physiological shock that may occur using other aqueous solutions. MRD is used extensively in food and environmental testing. The low level of peptone provides a protective effect but does not allow for multiplication during the short residence time in the diluent, 45 minutes. The sodium chloride prevents osmotic shock as the sample is initially diluted.
-
Maximum Recovery Diluent (Peptone/ Saline Diluent) An osmotically controlled solution for the preparations of suspensions of food samples and for use as a diluent in dilution techniques for bacterial enumeration. The presence of a low level peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringers Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent. -
Meat peptone is sourced from hydrolyzed proteins of animal tissue. This peptone consists of soluble amino acids and peptides that provide readily available nitrogen and other essential growth factors. It is primarily used for the cultivation of fastidious and non-fastidious microorganisms in microbiological culture media.
-
2 port media bag for use with large capacity liquid products Volume(s) : 10L -
-
2 port media bag for use with large capacity liquid products Volume(s) : 5L
-
Medical Flat Bottle with a 200ml volume capacity and a 28mm polypropylene cap Volume(s) : 200ml -
Membrane Lauryl Sulphate Agar is used for the enumeration of Escherichia coli and other coliforms found in filter membranes used in water sample testing. The broth base, originally named Membrane Enriched Teepol broth,(1) was updated when Teepol 610 was removed from the formulation and replaced by sodium lauryl sulphate.(2&3) The peptones, yeast extract and lactose act as carbon, nitrogen and vitamin sources in this medium. The phenol red is added as a pH indicator to detect the fermentation of lactose to differentiate the coliforms. Sodium lauryl sulphate is an inhibitory agent. References (1) Burman, N. P., 1967. Development of membrane filter techniques. II. Adaptation to routine and special requirements. Proc. Soc. Wat. Treat. Exam., 16:40 (2) Joint Committee of PHLS and the Standing Committee of Analysis. 1980. J. Hyg. Camb., 85:181 (3) Stanfield, G. and Irving, T. E., 1981. A suitable replacement for Teepol 610 in the selective isolation of coliforms from marine waters and sewage. Water Research. 15:469-474
-
Membrane Lauryl Sulphate Broth is used for the enumeration of Escherichia coli and other coliforms found in filter membranes used in water sample testing. Originally named Membrane Enriched Teepol broth,(1) this recipe was updated when Teepol 610 was removed from the formulation and replaced by sodium lauryl sulphate.(2&3) The peptones, yeast extract and lactose act as carbon, nitrogen and vitamin sources in this medium. The phenol red is added as a pH indicator to detect the fermentation of lactose to differentiate the coliforms. Sodium lauryl sulphate is an inhibitory agent. References (1) Burman, N. P., 1967. Development of membrane filter techniques. II. Adaptation to routine and special requirements. Proc. Soc. Wat. Treat. Exam., 16:40 (2) Joint Committee of PHLS and the Standing Committee of Analysis. 1980. J. Hyg. Camb., 85:181 (3) Stanfield, G. and Irving, T. E., 1981. A suitable replacement for Teepol 610 in the selective isolation of coliforms from marine waters and sewage. Water Research. 15:469-47
-
For the enumeration of Escherichia coli and coliform organisms in water using a Membrane Filtration Technique. Previously known as Membrane Enriched Teepol Broth, Lauryl Sulphate has replaced Teepol 610, which is no longer available. Phenol Red is included in the medium making it possible for coliforms to be more readily detected following incubation.
-
BM0016 micrAlert Coliforms is a selective medium for the detection, recovery, and isolation of coliforms from cleaned, disinfected inanimate surfaces. It may be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. Coliforms are facultatively anaerobic, Gram-negative bacteria that belong within the Enterobacteriaceae family. They are oxidase-negative, can ferment and produce gas from lactose when grown at 37°C, and express β-galactosidase activity. Escherichia coli can be further identified by producing growth at 44°C and indole from tryptophan. BM0016 micrAlert Coliforms is based on Minerals Modified Glutamate Medium, intended for enumerating coliforms from water samples. A medium containing glutamic acid was devised by Folpmers in 1948 (1) then further developed by Gray (2) in 1959 as a potential replacement for MacConkey Broth. In 1964, Gray then further modified the media to incorporate a variety of minerals to allow better lactose fermentation by coliforms and Escherichia coli (3). References 1. Folpmers, T. (1948) Is it justified to use Lactose Broth for the detection of Bact. coli in the presumptive test of routine water analysis. Leeuwenhoek ned. Tijdschr. 14: 58. 2. Gray, R.D. (1959) Formate lactose glutamate: a chemically defined medium as a possible substitute for MacConkey Broth in the presumptive coliform examination of water. J. Hyg. Camb., 57(3):249-265. 3. Gray, R.D. (1964) An improved formate lactose glutamate medium for the detection of Escherichia coli and other coliform organisms in water. J Hyg (Lond). 62(4):495-508. -
BM0014 micrAlert Listeria is a selective medium for the detection, recovery, and isolation of Listeria species from cleaned, disinfected inanimate surfaces. It should be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. BM0014 micrAlert Listeria is based on Half Fraser Broth, developed in 1988 by Fraser (1). Listeria monocytogenes is commonly found in soil, sewage, or faeces. It is difficult to eradicate and can cause serious food poisoning. L. monocytogenes is frequently tested for in food processing facilities to avoid contamination. Contamination can occur at all steps of the food manufacturing chain from raw materials to place of consumption. Confirmation that food processing surfaces are free from contamination with Listeria spp. after cleaning and disinfection is a critical step in preventing disease. Residual disinfectant actives may inhibit the growth of viable organisms preventing detection, so the neutralising agents contained in SW0006 micrAlert Neutraliser inactivate these residual disinfectants allowing improved recovery of sub-lethally damaged target organisms in BM0014 micrAlert Listeria. -
SW0006 micrAlert Neutraliser Swab Kit is a general-purpose neutralising buffer for use with micrAlert bacterial indicator products. The product is designed to neutralise the antimicrobial effects of disinfectants (1) allowing recovery of microorganisms sampled from surfaces that may contain disinfectant residues. The accompanying sterile swab is present to allow easy sampling of the target surface. This is a general-purpose, ready-to-use neutralising solution containing lecithin, polysorbate 80, histidine and sodium thiosulphate to inactivate disinfectants including hypochlorite bleaches, peroxy compounds, quaternary ammonium compounds, phenolics, alcohols, hexachlorophene, mercury products, halogens, and aldehydes (1). References 1. Russell AD. Neutralization procedures in the evaluation of bactericidal activity. In: Collins CH, Allwood MC, Bloomfield SF, Fox A, eds. (1981) Disinfectants: their use and evaluation of effectiveness. London: Academic Press, pp. 45-59. Tryptone is present to assist in recovery of non-lethally damaged organisms that may be present, sodium chloride provides osmotic balance, and pH is maintained by the phosphate buffer system. The sterile swab included in the kit allows for specimen collection from inanimate surfaces. -
BM0015 micrAlert Salmonella is a selective enrichment media for the isolation of Salmonella spp. from cleaned, disinfected inanimate surfaces. It should be used in conjunction with SW0006 micrAlert Neutraliser Swab Kit. BM0015 micrAlert Salmonella is designed to indicate the presence of Salmonella spp. on previously cleaned and disinfected surfaces. Salmonella spp. are frequently tested for on food processing surfaces to ensure the effectiveness of the cleaning/disinfection regimen and reduce the risk of food contamination. Salmonellae can decarboxylate lysine resulting in production of cadaverine, an alkaline amine capable of overcoming acid produced by fermentation thus maintaining a higher pH in the medium. Residual disinfectant actives may inhibit the growth of viable organisms preventing detection, so the neutralising agents contained in SW0006 micrAlert Neutraliser are designed to inactivate these residual disinfectants allowing improved recovery of sub-lethally damaged target organisms using BM0015 micrAlert Salmonella. -
Middlebrooks 7H10 Selective Medium is an Agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H11 Agar in that it has a lower concentration of Malachite Green, which is said by some workers to make it more suitable for primary isolation. The medium is complex but includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. Further enrichment is provided by the addition of Oleic Acid, Albumen and Dextrose and it is made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
Middlebrooks 7H11 Selective Medium is an agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H10 Agar in that it has a higher concentration of Malachite Green. The medium is complex and includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. The medium is also made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
This is a liquid medium for growing pure cultures of Mycobacterium spp., including M. tuberculosis, for use in antimicrobial assays and biochemical tests. The medium is complex but includes L-Glutamic acid, ammonium sulphate, sodium citrate, pyridoxine and biotin as growth factors as well as magnesium sulphate and ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. The medium is further enriched by the addition of the Middlebrook OADC Enrichment supplement. OADC contains oleic acid to provide fatty acids for growth promotion, bovine albumin and catalase as protective compounds as well as sodium chloride and dextrose.
-
This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis.
