Dehydrated Culture Media

Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms. Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated. With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance. For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

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  • Columbia agar is a general-purpose, non-selective medium capable of supporting the growth of most micro-organisms based on the original Columbia agar formulation described by Ellner et al. from Columbia University. With further enrichment using blood, most fastidious organisms can be isolated and their β-haemolytic reactions can be determined in order to aid identification. The peptone is the source of the required nitrogen, carbon and vitamins. Starch increases growth of Neisseria spp., and enhances the haemolytic reactions of some streptococci. This medium is relatively free of reducing sugars, which have been reported to adversely influence the haemolytic reactions of β-haemolytic streptococci . Supplementation of the base medium with blood (5- 10%) will provide additional growth factors for fastidious microorganisms, and aids in determining haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood and the type of basal medium used. Related Supplements : Defibrinated Horse Blood, Defibrinated Sheep Blood, Horse Blood Lysed, LS0008 Staph/Strep Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficle Selective SupplementLS0012 Bacitracin Selective Supplement, LS0011 Campylobacter Growth Supplement, LS0009 Campylobacter (Skirrow) Selective Supplement
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  • Sabouraud Dextrose Agar is one of several media available for theisolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud Dextrose Agar is a modification of a medium originally described by Sabouraud. The tryptone and meat peptone provide the nitrogen and vitamins required for organism growth in Sabouraud Dextrose Agar. The high concentration of dextrose is included as an energy source and in tandem with the acidic pH (5.6) facilitates the growth of fungi whilst providing limited selective properties. Higher levels of selectivity can be achieved against bacterial species by the addition of chloramphenicol. NB: This is a basic medium only and contains no additional supplements. Related Supplements : LS0050 Chloramphenicol Selective Supplement
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  • CLED SI Agar Cystine Lactose Electrolyte Deficient Single Indicator (CLED SI) Agar is based on Mackey and Sandy’s formulation and is popular for culturing urine specimens in the clinical laboratory. The reduced number of electrolyte level prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue as a pH indicator allows easy differentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement.  
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  • Bevis modified Mackey and Sandy’s original medium by introducing a double indicator to improve the differentiation of lactose and non-lactose fermenting coliforms, staphylococci and streptococci spp. Cystine Lactose Electrolyte Deficient Double Indicator (CLED DI) is popular for urine culture in the clinical laboratory. The reduced number of electrolytes prevents swarming of Proteus spp. The peptone and beef extract is the source of the required nitrogen, carbon and vitamins. Lactose is a carbohydrate. The inclusion of bromothymol blue and Andrade’s as indicators allows easy differentiation of lactose and non-lactose fermenting organisms. L-Cystine is also present to benefit those organisms that have a particular cystine requirement. Streptococcus pyogenes and many other fastidious organisms that do not require the presence of blood can grow on this medium.  
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  • Fastidious Anaerobe Agar is intended as a primary isolation medium capable of supporting most clinically significant anaerobes. The formulation is complex and includes detoxification agents (starch and sodium bicarbonate), growth enhancing agents (L-Cysteine, arginine, vitamin K, sodium succinate, glucose and pyrophosphate), as well as haemin to encourage pigment production in Bacteroides melaninogenicus. The peptone is the source of the required nitrogen, carbon and vitamins. Glucose also acts as a carbon source and sodium pyruvate as an energy source. Sodium chloride maintains the osmotic balance in the medium. Sodium pyrophosphate acts as a buffering agent and sodium succinate improves the growth of organisms such Bacteroides spp. Supplementation of the base medium with blood (5 - 10%) will provide additional growth factors for the more fastidious microorganisms, and aids in determining any haemolytic reactions. Related Supplements : LS0015 Actinomycete Selective Supplement, LS0017 Neomycin Selective Supplement, LS0022 Clostridium difficile Selective Supplement, LS0023 Clostridium perfringens Selective Supplement, BM0140 Egg Yolk Emulsion
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  • Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections (UTI). The peptone is the source of the required nitrogen, carbon and vitamins. Based on the traditional CLED medium, to prevent the swarming of Proteus spp., two chromogens are present in the medium. One allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and tryptophan are also included as indicators of tryptophan deaminase activity producing brown colonies of Proteus spp. Related Supplements : SHS500 Sterile Horse Serum 500ml
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  • Thiosulphate citrate bile salts sucrose (TCBS) agar is a selective medium used in microbiology laboratories to isolate Vibrio spp. The formulation was developed by Kobayashi et al. which was modified from Nakanishi’s formulation. The yeast extract and peptone are the source of the required nitrogen, carbon and vitamins. TCBS agar contains high concentrations of sodium thiosulphate, sodium citrate and ox bile to inhibit the growth of Gram-positive organisms and suppress coliforms. Sucrose is included as a fermentable carbohydrate for the metabolism of Vibrio spp. Sodium chloride maintains the osmotic balance in the medium. Sodium thiosulphate also serves as a sulphur source and, in combination with ferric citrate, detects hydrogen sulphide production. Thymol blue and bromothymol blue are included as indicators of pH changes.
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  • This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with charcoal, ferrous sulphate and sodium pyruvate replacing the horse blood. The medium is made selective by the addition of vancomycin and cefsulodin, to suppress other bacteria, and amphoteracin to inhibit yeasts. Horse serum (10%) is also added to promote optimum growth of Helicobacter spp. Related Supplements : LS0031 Helicobacter pylori Selective Supplement, SHS500 Sterile Horse Serum 500ml
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