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This medium was initially intended for the selective isolation of Shigella spp. but can also be used for Salmonella spp. primarily from clinical specimens although it has been used in the examination of dairy products. The medium contains high levels of Peptone to counteract some of the toxic effects, particularly on Shigella, of the Bile Salts used to make the medium selective. In addition to Lactose, Sucrose and Salicin are also included allowing for improved differentiation than with Lactose only. The presence of H2S is detected by the reaction between Ammonium Ferric Citrate and Sodium Thiosulphate. A double indicator system of Acid Fuchsin and Bromothymol Blue is helpful in the differentiation process. -
Helicobacter Pylori Medium with 10% Horse Serum, Cefsulodin (10mg/L), Vancomycin (10mg/L) & Amphoteracin (20mg/L) This is a selective medium for the isolation of Helicobacter pylori from clinical samples. The medium is based on a modification of Campylobacter CCDA Blood Free Medium with Charcoal, Ferrous Sulphate and Sodium Pyruvate replacing the Horse Blood and is made selective by the addition of Vancomycin and Cefsulodin to suppress other bacteria and Amphoteracin to inhibit yeasts. 10% Horse Serum is also added to promote optimum growth of helicobacter. -
Helicobacter pylori Selective Supplement E&O Laboratories Ltd Helicobacter pylori Selective Supplement can be used with Helicobacter pylori Base Agar (E&O product KM0009).This selective supplement is composed of 2 antibiotics and one antifungal agent. These agents inhibit the background flora and allow the cultivation of Helicobacter pylori. Helicobacter pylori, previously named Campylobacter pylori, is a Gram-negative, microaerophilic bacterium found in the stomach, and may be present in other parts of the body, such as the eye. -
Lysed horse blood is used for special purposes in culture media. It has been used for many years in Corynebacterium diphtheriae media, where better growth was observed after lysis of the horse blood by the tellurite in the medium. It is also documented that lysed blood stimulates the growth of Haemophilus influenzae due to the release of Nicotinamide Adenine Dinucleotide in the horse blood from the ruptured erythrocytes. In antibiotic susceptibility testing, lysed horse blood is added to the medium to improve the reactions with trimethoprim and sulphonamides. Most culture media, unless specially processed for susceptibility testing, contain amounts of thymidine which can antagonise the inhibitory effects of these antimicrobials. When horse blood is lysed the erythrocytes release an enzyme thymidine phosphorylase which converts thymidine into the much less antagonistic compound thymine. -
A selective culture medium for the isolation and differentiation of Corynebacterium diphtheriae types, Hoyle’s medium allows for rapid growth of the organisms and normally 18 hours incubation should be sufficient for a diagnosis. As the medium is highly selective, inoculation should be by rubbing the swab (or other material) over the entire surface of the agar, there is no need to spread the inoculum with a loop indeed doing so can cause the organism to be missed especially when they are present only in small numbers. -
Iso-Sensitest with 5% Horse Blood & NAD (20mg/L) (25ml per dish) This is a defined medium suitable for antimicrobial susceptibility testing and on which most organisms will grow. The medium has been enriched with Horse Blood to meet the demands of the more fastidious organisms and NAD (Nicotinamide Adenine Dinucleotide) is also included to further enhance the growth of Haemophilus spp. This medium is included in the recommendations of BSAC as being appropriate for the susceptibility testing of Haemophilus spp. -
This is a selective medium for the isolation and enumeration of enterococci (Group D streptococci) primarily in food although it has found uses in other areas of bacteriology. The medium is made selective by the inclusion of Kanamycin and Sodium Azide while Aesculin and Ferric Ammonium Citrate act as the indicator system. -
Kanamycin Selective Supplement (50mgs/L) E&O Laboratories Ltd Kanamycin Selective Supplement can be used with LB Agar for selective cultivation of E. coli and other organisms that contain plasmids conferring kanamycin resistance. Kanamycin is an aminoglycoside bacteriocidal antibiotic used to treat a wide variety of infections Kanamycin is isolated from the soil bacterium Streptomyces kanamyceticus.
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Kirchner medium is a liquid medium for the selective enrichment and isolation of Mycobacteria spp from clinical specimens, particularly when the organisms may be present only in small numbers (e.g. CSF and tissue biopsies). This medium has been enriched by the addition of calf serum as it is a widely used supplement because it is rich in growth factors. The medium is made selective by the inclusion of Polymyxin B, Ticarcillin and Trimethoprim to inhibit other bacteria and Amphotericin B to inhibit yeasts and fungi. It is generally used in conjunction with a solid medium such as Lowenstein Jensen Medium. Specimens from normally sterile body sites may be inoculated without digestion and decontamination. Other specimens should be pre-treated according to standard procedures. The inoculated medium should be incubated at 35-37°C for up to 8 weeks. Kirchner medium should be used in conjunction with a solid medium (e.g. Lowenstein-Jensen medium) in order to accelerate differentiation and identification testing. -
This is a modification of the traditional liquid media used to differentiate and identify micro-organisms. It consists of a Buffered Tryptose base containing Lactose and solidified with Gelatin which permits detection of Gelatin Liquefaction where appropriate. Phenol Red Indicator is also included as an indicator of pH change. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of carbenicillin at 100mg/L for use with transformed cells harbouring selection plasmids containing carbenicillin resistance genes. Peptides and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This media has the addition of Kanamycin at 0.05gms/L for use with Kanamycin resistant strains and cells harbouring selection plasmids containing the Kanamycin resistance gene.Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. -
LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s.These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. This medium has the addition of kanamycin at 50mg/L and chloramphenicol at 34mg/L, for use with kanamycin and chloramphenicol resistant strains and cells harbouring selection plasmids containing the kanamycin and rifampicin resistance genes. Peptides, peptones and essential amino acids are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract and sodium chloride provides osmotic balance. -
Legionella Cystine Free Medium is intended for use in conjunction with Legionella CYE Medium (PP0200) as a secondary diagnostic medium for confirmation of a previously isolated organism. Although it contains Ferric Pyrophosphate and α-ketoglutarate it does not contain any L-Cysteine Hydrochloride. NB : This is a base medium only and will not sustain the growth of Legionella spp. -
E&O Laboratories Ltd Legionella Growth Supplement (LS0053) is an enrichment supplement used to enhance the isolation of Legionella spp. in combination with Charcoal Yeast Extract (CYE) agar. CYE Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of containing the bacteria.
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This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram negative organisms and Cycloheximide is also included to inhibit yeasts and fungi. -
This is a selective medium for the isolation of Legionella spp used primarily in clinical and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-Cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer and Potassium hydroxide are incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Cefamandole and Polymixin to inhibit most gram positive and gram negative organisms and Cyclohexamide is also included to inhibit yeasts and fungi. -
E&O Laboratories Ltd Legionella BMPA Supplement (LS0156) is used in the identification of suspected colonies of Legionella spp. when used to prepare Legionella BMPA agar. Legionella BMPA Supplement contains cefamandole and polymyxin B to inhibit most Gram-positive and Gram-negative organisms.
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This is a basic medium for the cultivation of Legionella spp. It is intended primarily for use as a secondary diagnostic medium in conjunction with Legionella Cystine Free Medium (PP0201) for confirmation of a previously isolated organism. NB: This is a basal medium only and although it will sustain the growth of Legionella spp. It contains no selective supplements. It is therefore not recommended as a means of primary isolations from clinical samples. -
E&O Laboratories Ltd Legionella GVPC Selective Supplement (LS0052) is used in the culture of suspected colonies of Legionella spp. Colonies of Legionella should be visible after 2 to 5 days incubation on BCYE agar with growth supplement and appear light blue to blue-grey in colour. Colonies suspected of being Legionella should be Gram-stained and subcultured to a fresh Legionella agar plate and to a Legionella agar plate not containing L-Cysteine. Gram-negative organisms that grow on Legionella Agar but fail to grow on Legionella agar (without L-Cysteine) may be presumptively identified as Legionella species. Definitive identification is performed on the basis of growth, morphology, and biochemical and immunological reactions. Appropriate references should be consulted for further information on identification procedures.
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Organisms Ref. No Result Legionella pneumophilia NCTC 11192 Growth, grey-white colonies Legionella anisa NCTC 11974 Growth, grey-white colonies Legionella bozemanae NCTC 11368 Growth, grey-white colonies Escherichia coli NCTC 12241 Inhibited Enterococcus faecalis NCTC 12697 Inhibited Pseudomonas aeruginosa NCTC 12903 Inhibited -
LIM Broth with 10% Serum A nutritious, selective broth medium utilising the base formulation developed by Todd and Hewitt for the enrichment of Group B Streptococci. The LIM broth is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of other bacteria. Serum is also included to enhance the nutritional qualities of the base medium. -
One of several media available as a selective identification medium for the isolation and identification of Listeria monocytogenes in food & clinical laboratories. Using Columbia Agar as the base Lithium Chloride is included to inhibit enterococci and Acriflavine to inhibit some other gram positive and gram negative organisms that may be present in such specimens. It is made further selective by the addition of the antimicrobials Cefoxitin, Colistin & Fosfomycin with Amphotericin included to inhibit any yeasts present. Aesculin is present as an indicator since Listeria monocytogenes will hydrolyse it and the associated reaction with the Ferric Ammonium Citrate gives rise to a black precipitate around the colonies. -
Listeria Oxford Selective Supplement E&O Laboratories Ltd Listeria Oxford Selective Supplement (LS0030) is a selective mixture used to supplement Listeria Oxford agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from clinical and food samples.
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This is a selective medium for the isolation and differentiation of bile tolerant gram-negative (enteric) and gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology. It has the disadvantage that many strains of Proteus spp will spread on it and for this reason MacConkey Agar without Salt may be preferred. -
This is a selective medium primarily for the isolation and differentiation of coliforms and non-lactose fermenting Gram –ve bacterial species from clinical, dairy, industrial and water samples. This media differs from the original MacConkey formulation with the addition of an extra 0.5% Sodium chloride, an altered Bile salt mix and the neutral red concentration was also modified. This enhanced the effect of inhibiting Gram +ve and other non-enteric bacterial species. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
This is a selective medium primarily for the isolation of Enterobacteriacae from waters & sewage. This media differs from the original MacConkey formulation in that as well as Bile Salts, Crystal Violet has been included as an additional selective agent. This has the effect of inhibiting gram-positive micrococci. -
Originally introduced for the isolation and differentiation of Lactose & Non-Lactose Fermenting enteric organisms the medium in this case has been modified to improve the isolation of staphylococci and enterococci. The absence of Sodium Chloride provides a low electrolyte medium that prevents spreading of most Proteus spp. Although recommended for use in the examination of urine samples in clinical laboratories it has uses in Food, Water and Dairy applications. -
This is a selective medium for the isolation of ESBL (Extended Spectrum Beta-Lactamase) producing strains of Escherichia coli. It should be noted that AmpC isolates may also be detected on this medium whilst non - ESBL organisms will be inhibited on this medium. The inclusion of bromocresol purple indicator displays the acid production due to the lactose fermentation by means of a colour change from purple to yellow. N.B. – This is double strength broth.
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This is a medium for the cultivation of yeasts and moulds. The high carbohydrate content is said to ensure rapid growth while the low pH (5.4) inhibits most bacteria. Malt Extract Agar can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. -
A selective medium for the isolation of Staphylococcus aureus. The high level of Sodium Chloride inhibits most other organisms and as most Staphylococcus aureus ferment Mannitol the inclusion of Phenol Red indicator gives rise to yellow colonies. This formulation complies with the requirements of the Harmonised USP/EP/JP. NB: Some strains of coagulase negative staphylococci can give rise to false positive results on this medium It is necessary therefore to confirm the identity of Staphylococcus aureus by other confirmatory tests (e.g. Coagulase test, Latex test etc.) -
For the enumeration of Escherichia coli and coliform organisms in water using a Membrane Filtration Technique. Previously known as Membrane Enriched Teepol Broth, Lauryl Sulphate has replaced Teepol 610, which is no longer available. Phenol Red is included in the medium making it possible for coliforms to be more readily detected following incubation.
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Middlebrooks 7H10 Selective Medium is an Agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H11 Agar in that it has a lower concentration of Malachite Green, which is said by some workers to make it more suitable for primary isolation. The medium is complex but includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. Further enrichment is provided by the addition of Oleic Acid, Albumen and Dextrose and it is made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
Middlebrooks 7H11 Selective Medium is an agar based medium for the isolation of Mycobacteria spp from clinical specimens. It differs from Middlebrooks 7H10 Agar in that it has a higher concentration of Malachite Green. The medium is complex and includes L-Glutamic Acid, Ammonium Sulphate, Sodium Citrate, Pyridoxine and Biotin as growth factors and Magnesium Sulphate, Ferric Ammonium Citrate as sources of trace ions. The medium is also made selective by the inclusion of Ticarcillin, Polymixin B, Trimethoprim and Amphotericin B. As with egg media Glycerol is included to enhance the growth of the Mycobacteria and Malachite Green is incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. -
This is a liquid medium for growing pure cultures of Mycobacterium spp., including M. tuberculosis, for use in antimicrobial assays and biochemical tests. The medium is complex but includes L-Glutamic acid, ammonium sulphate, sodium citrate, pyridoxine and biotin as growth factors as well as magnesium sulphate and ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. The medium is further enriched by the addition of the Middlebrook OADC Enrichment supplement. OADC contains oleic acid to provide fatty acids for growth promotion, bovine albumin and catalase as protective compounds as well as sodium chloride and dextrose.
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This is an agar-based medium for the isolation of Mycobacterium spp. from veterinary samples; particularly the species primarily responsible for bovine TB, M.bovis. The medium is complex but includes L-Glutamic acid, Ammonium sulphate, Sodium citrate, Pyridoxine and Biotin as growth factors and Magnesium sulphate, Ferric ammonium citrate as sources of trace ions. Di-sodium phosphate and Mono-potassium phosphate are also present to maintain the pH of the medium. Further enrichment is provided by the addition of Oleic acid, Albumin and Dextrose. The medium is made selective by the inclusion of Ticarcillin, Polymyxin B, Trimethoprim and Amphotericin B. Malachite green is also incorporated to provide a colour contrast between the colonies and the medium as well as contributing some inhibitory effect on organisms other than mycobacteria. It should be noted that Glycerol is NOT added to this medium as Glycerol can be inhibitory for M.bovis when examining veterinary samples. However, this product contains Lysed sheep blood, Adult bovine serum and Sodium pyruvate to enhance the growth of M.bovis. -
This medium is used for the presumptive identification of Pseudomonas aeruginosa from water and environmental samples. Pseudomonas aeruginosa is presumptively identified by the characteristic green pigmentation of the colonies with hydrolysis of casein (clear zones around each colony). -
A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. This medium allows for the detection of gas when a Durhum tube is incorporated in the medium. -
A broth suitable for the cultivation of bacteria especially members of the Enterobacteriacae genus. Which allows the detection of acid & gas when a Durhum tube is incorporated in the medium. -
Modified Campylobacter (Bolton) Selective Supplement E&O Laboratories Ltd Campylobacter Modified (Bolton) Selective Supplement (LS1054) is a supplement used to enhance the selective isolation of Campylobacter spp. primarily from clinical specimens.
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Modified Semi Solid Rappaport Vassiliadis Medium (MSRV) is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in faeces and food products. The original research on MSRV Medium revealed a semi-solid could be used as a rapid and sensitive test for isolating motile Salmonella spp. from food products following pre-enrichment or selective enrichment. The semi-solid medium allows motility to be detected as halos of growth around the original point of inoculation.
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This is a solution of novobiocin used as the selective agent in Modified Semi Solid Rappaport Vassiliadis Medium (BM4031) for the detection of motile Salmonella spp.
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Mueller Hinton Agar is recommended for use in the antibiotic disk diffusion method by both the European Committee on Antibiotic Sensitivity Testing (EUCAST) and the Clinical Laboratory Standards Institute (CLSI). The medium contains low levels of divalent metal cations, such as calcium and magnesium, to minimise any interference with certain antibiotic classes e.g. aminoglycosides. Starch is also present to absorb any toxic metabolites that may be formed during growth. The medium is low in thymine & thymidine content and is therefore suitable for use in testing sulphonamides and trimethoprim without the addition of blood. -
Mueller Hinton Agar Chocolate is used for the isolation and cultivation of fastidious bacteria from clinical specimens. It may also be used for the susceptibility testing of Neisseria gonorrhoeae. Mueller Hinton Agar Chocolate is an enriched, non-selective medium on which fastidious and non-fastidious bacteria, including normal flora, will grow. Therefore, it is recommended to inoculate specimens also onto appropriate selective media. The term “fastidious bacteria” relates to bacteria that do not grow or do not grow well on normally used primary isolation media containing sheep blood. -
Approved by the Clinical Laboratory Standards Institute (CLSI) in USA this medium can be considered as an alternative to Iso-Sensitest Agar for antimicrobial sensitivity testing by disc diffusion methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim without the addition of Lysed Blood. -
Approved by the National Committee for Clinical Laboratory Standards (NCCLS) in USA this medium is approved for use in antimicrobial sensitivity testing by the disc diffusion method and is recommended particularly for use with the Bauer-Kirby Technique It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim and controlled to ensure correct zone sizes with Tetracyline and Aminoglycoside antibiotics. It can be considered as an alternative to Iso-Sensitest Agar. This particular formulation has an additional 2% Sodium Chloride added to the medium making it suitable for the detection of resistance to Methicillin in staphylococci and it is included in the recommendations of British Society for Antimicrobial Chemotherapy (BSAC) for this purpose. It is not however recommended for testing of organisms requiring a CO2 enriched environment due to the pH effect on the medium. If incubation in a CO2 enriched environment is essential control organisms should be included to confirm that results have not been altered. -
Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST). This medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Additional supplementation of the Mueller Hinton medium using 5% Horse Blood and 20mg/L of Nicotinamide adenine dinucleotide (NAD) makes it suitable for use with the more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae. -
Approved by the Clinical & Laboratory Standards Institute (CLSI formerly known as the NCCLS) in USA this medium can be considered as an alternative to Iso-Sensitest Broth for antimicrobial sensitivity testing and MIC determinations by tube dilution methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim. It is sometimes used in conjunction with Mueller-Hinton Agar. -
Mueller-Hinton with 2% Glucose & Methylene Blue (25ml) This medium is intended for use as a means of differentiation of Candida spp. based on Mueller-Hinton Agar base. The medium is modified by the addition of Glucose and Methylene Blue indicator and is the recommended media for the susceptibility testing of Yeasts according to the CLSI M44-A2 document. -
This is a selective enrichment broth for the isolation of Salmonella spp. primarily from food and food product samples and conforms to the requirements ISO 6579:2002. It can however be used in other areas including clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. Immediately prior to use it is necessary to add 200µl of 2% Iodine/Iodide Solution (BM0946 - Supplied with the medium). Once the Iodine/Iodide Solution has been added the medium should be used immediately and cannot be stored for future use. NB: As this is an opaque medium, the turbidity of the broth alone cannot be used as an indication of growth. -
This is a selective enrichment broth for the isolation of Salmonellae spp. primarily from food and food product samples and conforms to the requirements as described in ISO 6579:2002. It can however be used in other areas including for clinical and environmental specimens. Salmonella reduce Tetrathionate and will proliferate in the medium whilst most other enteric organisms are inhibited. Unlike the older traditional Tetrathionate Broth the addition of Novobiocin (40mg/L) improves the inhibition of Proteus spp. This complete medium already includes the 2% Iodine solution that is traditionally added immediately before use. NB: As this is an opaque medium turbidity cannot be used as an indication of growth. -
A selective medium for the isolation of fungi, particularly dermatophytes from clinical specimens, Mycological Agar is suitable for use in all areas of Mycology. The medium inhibits most bacteria due to the addition of Chloramphenicol which is added to reduce the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
Based on Fastidious Anaerobe Agar Base with added Egg Yolk Emulsion, this medium can be used to test Clostridium perfringens for phospholipase production. A zone of opalescence around the colonies is indicative of a positive reaction. It can also be used as an aid to identification of Clostridium perfringens if antitoxin is spread onto half of the plate prior to inoculation (Nagler Reaction). -
Neomycin Selective Supplement E&O Laboratories Ltd Neomycin Selective Supplement (LS0017) is an antibiotic supplement used to enhance the isolation of anaerobes from clinical specimens.
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Pages Amoeba Saline & Agar No.1 This is a non-nutrient medium based on Page’s Amoeba Saline, a buffered salt solution, solidified with 1.5% Agar. -
Novobiocin Supplement (20mgs/L) E&O Laboratories Ltd Novobiocin Selective Supplement can be used to enhance the isolation of Salmonella spp. by motility enrichment and the selective enrichment of E.coli Serogroup O157 in food and faeces samples and also in the isolation of Salmonella typhimurium at 40mgs/L when used with Muller-Kauffmann Tetrathionate (MKTTn) Broth.
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A basic general-purpose medium suitable for use in the cultivation of the less fastidious organisms particularly those that do not require the addition of blood or other enrichment. -
Oxytetracycline (100mgs/L) Selective Supplement E&O Laboratories Ltd Oxytetracycline Selective Supplement can be used with a glucose, yeast or maltose extract based agar for selective isolation of Yeasts and Moulds from a variety of sample types. Due to the near neutral pH of the medium, Oxytetracycline is added to reduce the risk of bacterial growth when processing materials that may be more heavily contaminated. Media using oxytetracycline as the selective agent is based on the formulation developed by Mossel et al. who stated that the use of this antibiotic in a medium with a neutral pH gave increased counts of yeasts and moulds from a variety of foodstuffs compared with media which relied on a low pH to suppress bacterial growth.
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This is an established medium, with a neutral pH, used for the enumeration of Yeasts and Moulds -
PALCAM Selective Supplement E&O Laboratories Ltd PALCAM Selective Supplement (LS0038) is a selective mixture used to supplement PALCAM agar base in order to facilitate the isolation of Listeria monocytogenes and other Listeria species from food samples.
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Based on the formulation of Dulbecco Solution ‘A’ this balanced salt solution with added Tween is intended for use primarily in Tissue Culture techniques. It can be used either on its own or with the addition of Calcium and Magnesium salts (Dulbecco Solution B). -
Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar) This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation. -
This medium is recommended for the detection and enumeration of yeasts and fungi in a variety of sample types. The low pH (5.6) and addition of streptomycin will ensure that the growth of most bacterial species is inhibited and the low mineral content ensures good pigment production by fungi where appropriate. -
Chromogenic Coliform Agar (CCA) Chromogenic Coliform Agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique. The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide. Salmon-GAL is used for the detection of β-D-galactosidase enzymatic activity. X-glucuronide is used for the detection of β-D-Glucoronidase enzymatic activity. β-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies. Unlike other coliforms, Escherichia coli leaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colonies. Tryptophan is used to increase detection reliability by improving the indole reaction. The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms. Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance. The selectivity is attained by the addition of Tergitol® 7 as it inhibits the growth of Gram positive bacteria. -
Primary Listeria Selective Supplement E&O Laboratories Ltd Primary Listeria Selective Supplement (LS0210) is a selective mixture used to supplement Primary Listeria Selective Agar (E&O PP7025) in order to facilitate the isolation of Listeria monocytogenes in foodstuffs and other samples.
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Traditionally, membrane Lauryl Suphate Broth (mLSB) was used as the standard media for isolating coliforms (including E. coli) from drinking water. Primary membrane Lactose Glucuronide Agar (mLGA) is a chromogenic modification of mLSB formulation aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and <em,>E. coli . The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. X-glucuronide is incorporated to allow for the presumptive isolation of E. coli, sodium pyruvate aids recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads. This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Environment Agency’s - The Microbiology of Drinking Water 2009 (Part 4). -
Tryptone Bile X (TBX) - Glucuronide Agar Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available. -
Side 1: Primary UTI Chromogenic Agar This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of enterococci (turquoise colonies), Proteus spp (clear colonies with a brown halo), Enterobacter spp (metallic blue colonies), staphylococci (white colonies), and E. coli (purple colonies). Side 2: Columbia Agar w 7% Defibrinated Horse Blood & CNA This is a selective medium for the isolation of Staphylococcus/ spp and Streptococcus spp. It is based on Columbia Agar enriched with defibrinated horse blood which promotes good colony appearance, pigment production and excellent haemolysis from beta-haemolytic streptococci. The medium is made selective by the inclusion of colistin and nalidixic acid to suppress growth of the majority of Gram-negative bacteria. -
This is a medium for the isolation and identification of Group B streptococci. The principal of the medium is based on the ability of group B streptococci to produce unique orange/red pigmented colonies when incubated anaerobically, particularly on media containing starch products. This medium is non-selective so other organisms will grow on this medium but they do not produce the characteristic pigment. -
Pseudomonas Agar Base with 1% Glycerol, Cephalothin, Fucidin & Cetrimide (CFC) This is a selective medium for the isolation of Pseudomonas spp primarily in food, water and environmental samples. The medium uses Magnesium and Potassium salts to enhance pigment production and is made selective by the addition of CFC supplement. The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism. -
Pseudomonas Agar Base with 1% Glycerol & CN Supplement A selective medium for the isolation of Pseudomonas aeruginosa the medium is made selective by the inclusion of Cetrimide and Naladixic Acid (CN) supplement to significantly reduce the enteric organisms particularly Proteus and Klebsiella spp. Magnesium and Potassium salts are included to enhance the production of the pigments pyocyanin and fluorescein. -
Pseudomonas CFC Selective Supplement E&O Laboratories Ltd Pseudomonas CFC Selective Supplement (LS0026) is an antibiotic supplement used to enhance the selective isolation of pseudomonads primarily from food and environmental samples.
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E&O Laboratories Limited Pseudomonas Selective CN Supplement (LS0006) is an antibiotic supplement used to enhance the selective isolation of Pseudomonas species particularly Pseudomonas aeruginosa.
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A selective medium for the isolation of Pseudomonas spp. primarily in food, water and environmental samples the medium is made selective by the addition of CFC supplement (cetrimide, at a concentration of 10mg/L which is said to allow the growth of all pseudomonads, cephalothin and fucidin). The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism. -
R2A medium was developed to determine the bacterial count including heterotrophic bacteria in potable waters during treatment and distribution. This medium has a low nutritional content and therefore requires extended incubation times. It is recommended by the Environmental Agency, Methods for the Examination of Waters and Associated Materials, and Standard Methods for the Enumeration of Water and Wastewater. -
Rappaport Vassiliadis (R.V.) Single Component Enrichment Broth This is an alternative to Selenite and Tetrathionate broths, as a selective enrichment broth for the isolation of Salmonellae spp from food, dairy and environmental samples and is claimed by some workers to be superior to both these formulations. It can also be used in clinical bacteriology but care must be taken to ensure that only a light inoculum is used. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms but allow salmonellae to multiply freely. NB: This media is not recommended for use when salmonella typhi is suspected. -
This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium. It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP. -
RPMI Medium for E-Test RPMI Medium is recommended for use in anti-fungal susceptibility testing of yeasts from clinical isolates using the E-Test method. The medium is based on a simple Glucose Agar with added RPMI-1640 Medium (without Sodium Bicarbonate & Phenol Red), which supplies the necessary vitamins and amino-acids, and MOPS (3-(Morpholino)propanesulfonic Acid) Buffer to maintain the medium pH during incubation. -
Sabouraud Dextrose Agar with Chloramphenicol (0.5g/L) is a selective media for the isolation of yeasts and fungi suitable for use in all areas of mycology. Sabouraud dextrose agar is a modification of a medium originally described by Sabouraud.(1) The tryptone and meat peptone provides the required nitrogen, carbon and vitamins. The high concentration of dextrose is included as an energy source. Due to the higher pH of the medium, an increased concentration of chloramphenicol is included to improve the selectivity of the media and inhibit a range of Gram-positive and Gram negative bacteria. 1. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061. -
This is one of several media available for the selective isolation of yeasts and fungi suitable for use in all areas of Mycology. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. NB: This is a basic medium only and contains no additional supplements. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia & European Pharmacopoeia. This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy -
Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) A selective medium for the isolation of yeasts and fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria, however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. -
Sabouraud Dextrose Agar with Chloramphenicol (50mg/L) & Cyclohexamide (Actidione) (300mg/L) A selective medium for the isolation of fungi, Sabouraud Dextrose Agar is suitable for use in all areas of Mycology. The low pH of the medium inhibits most bacteria however in this formulation Chloramphenicol (50mg/L) is added to further reduce the risk when processing material that may be heavily contaminated particularly with coliforms. Cycloheximide is also added to suppress the growth of yeasts and saprophytic fungi. -
This is a modification of Sabouraud Dextrose Agar. The low pH (5.6) of the medium is inhibitory to most bacteria and it has been made specifically selective by the addition of colistin and gentamicin. This further reduces the risk of bacterial contamination when processing material that may be heavily contaminated particularly with Gram negative organisms such as Pseudomonas aeruginosa. -
This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin & Polysorbate 80 (Tween 80) are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds & Tween neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol). NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
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Sabouraud Dextrose Agar with Lecithin, Tween 80, Histidine & Sodium Thiosulphate (VHP) (USP) - Irradiated This is a selective medium for the isolation of yeasts and fungi and is particularly suitable for use in sterility testing. The low pH (5.6) of the medium is inhibitory to most bacteria and many diagnostic features such as spores and pigmentation are well developed on this medium. The formulation of this medium conforms to the requirements of the United States Pharmacopoeia. Lecithin, Polysorbate 80 (Tween 80), Histidine and Sodium Thiosulphate are added to inactivate surface disinfectants (Lecithin neutralises quaternary ammonium compounds, Tween 80 and Histidine neutralises phenols, formalin, hexachlorophene and in combination with the Lecithin ethanol and Sodium Thiosulphate inactivate mercurials). This product is wrapped in barrier film to allow for use in Vaporised Hydrogen peroxide sterilisation systems. NB: Final sterilisation of this medium is by Gamma irradiation and it is triple wrapped. Dose Range: Min: 8.0kGy Max: 15.0kGy
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This product is primarily used as a fixation agent in immunological complement fixation assays. The Sodium citrate acts as an anticoagulant in the Alsever’s solution once it is combined with the sheep blood. -
Originally intended as a medium for the enumeration of enterococci in water using Membrane Filtration, this medium has become more popular in many other areas such as food bacteriology. The medium contains Tetrazolium Chloride, which is reduced by enterococci to the insoluble red dye Formazan resulting in dark red colonies of enterococci on the agar. It should be noted that this reaction is not exclusive to enterococci and colonies should be confirmed by additional testing e.g. Aesculin hydrolysis. -
This is a differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey media in that lactose has been replaced by sorbitol. As Escherichia coli 0157:H7 does not ferment sorbitol, it produces pale translucent colonies whereas most other strains of Escherichia coli do ferment sorbitol and produce pink colonies. -
Sorbitol MacConkey with Cefixime & Tellurite (CT-Smac) This is a selective differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey medium in that Lactose has been replaced by Sorbitol. As Escherichia coli 0157:H7 does not ferment Sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are Sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial Cefixime and Potassium Tellurite. -
Staph/Strep Selective Supplement E&O Laboratories Ltd Staph/Strep Selective Supplement (LS0008) is an antibiotic supplement used to enhance the selective isolation of Staphylococci and Streptococci species.
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This medium is utilised for the transportation and cryopreservation of Streptococcus pneumoniae & Neisseria meningitidis isolates.
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Thiosulphate Citrate Bile Salts Sucrose (TCBS) Agar TCBS is a selective isolation medium for culture of pathogenic Vibrio spp. from clinical samples. The formulation was developed by Kobayashi, Enomoto, Skazaki and Kuwahara. This medium inhibits most enterobacteriacae for at least 24 hours. For the isolation of Vibrio spp. other than V.cholerae in environmental bacteriology, it is advisable to incubate at the lower temperature range of 20°C – 30°C. NB - It is not recommended to perform an oxidase test on any presumptive positive isolates directly from TCBS medium. -
This is a medium to detect Thermo-Stable-Nuclease from Staphylococcus aureus after heat inactivation of the organism. After boiling and centrifugation the supernatant is placed in a well in the plate and incubated for 4 hours. If present, the enzyme breaks down the DNA in the medium and produces a zone of clearing indicating a positive reaction. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of the broad spectrum antibiotic, chloramphenicol, to inhibit a wide range of Gram-positive and Gram-negative bacterial species. This medium does not contain an antifungal agent and Candida spp. will not be suppressed. However, the growth of Candida spp. does not interfere with that of Trichomonas spp. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours. -
This medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. The medium is selective due to the inclusion of two selective agents namely, Chloramphenicol and Nystatin, to inhibit a wide range of both Gram-positive and Gram-negative bacterial species as well as yeasts and fungi. The inclusion of methylene blue as a redox indicator allows for the visualisation of any significant oxygen diffusion in the medium. Cultures may be examined microscopically after 48 hours incubation at 37°C for the presence of flagellate protozoans. If a negative result is obtained then the culture may be re-incubated for a further 72 hours.
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Triphenyltetrazolium Chloride Soya Tryptone (TSAT) Agar Complete Triphenyltetrazolium Chloride (TTC) has been added as an indicator to various media, and recommended by several workers as being helpful in the early recognition and identification of a variety of bacteria including Escherichia coli, Vibrio parahaemolyticus and enterococci. This particular formulation is based on a Tryptone Soya Agar with added Sucrose and is particularly useful when performing counts on food and food product samples. Many of the enterobacteriaceae and enterococci will reduce the TTC to a formazan which colours the colonies deep red making them easier to distinguish and identify. The presence of the Sucrose can also assist in the differentiation of Sucrose fermenting and non-fermenting strains. -
This is a medium that can be used to differentiate between some of the Enterobacteriacae on the basis of four reactions, fermentation of Lactose, Glucose and Sucrose and the production of H2S. For use the medium is inoculated using a pure culture of the test organism which should be smeared onto the surface of the slope and stabbed into the butt of the medium. For details of the many reactions that may arise during the use of this medium reference should be made to one of the many standard textbooks.
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This is a plate count agar originally suggested by the American public Health Association for the estimation of total viable counts in food and dairy products.
